摘要： 为了筛选适合安徽优质烟区且对烟草青枯病更具拮抗抑菌活性作用的拮抗菌，采用光密度法和平板计数法分别测定了3种烟草青枯病拮抗菌发酵液的拮抗抑菌效应和影响因子进行研究。结果表明：3种拮抗菌均具有较好的拮抗活性。5，10，20，40，80倍5个浓度梯度的3种拮抗菌都比对照（CK）的光密度值（OD）高，均高于57个百分点以上，且达到了显著水平；且拮抗抑菌顺序依次为AKJK-2013-02 > AKJK-2013-13 > AKJK-2013-11。3种拮抗菌均能在开始生长时就能分泌抗菌物质，且拮抗抑菌活性与发酵时间呈成正相关；3种拮抗菌的拮抗抑菌活性都随着存放时间的增加而减弱，且均在存放28 d后的拮抗抑菌活性还能达到83.5%以上。因此，3种烟草青枯病拮抗菌具有一定的开发价值和应用前景。

关键词: 烟草青枯病, 拮抗菌, 发酵液, 抑菌活性

Abstract: To select the antagonistic bacterial strain that suited for high quality tobacco area of Anhui,the study on the antagonistically antibacterial effects of three kinds of antagonistic fermentation and the factors by the broth optical density and plate count.The results were that:three antagonistic strains had the better antibacterial effects;5,10,20,40,80 times concentration gradients had high optical density(OD) than the control(CK),and over 57%,reached to a significant level;The antagonistic order was AKJK-2013-02 > AKJK-2013-13 > AKJK-2013-11. The antibacterial effects were positively correlated with the fermentation time,and were weakened increasing storage time,and over 83.5% after storage 28 d.Three antagonistic strains had broth certain development values and application prospects.

Key words: Tobacco bacterial wilt, Antagonistic bacteria, Fermentation, Antibacterial effect

中图分类号: 

• S435.711