拮抗小麦赤霉病内生细菌YB-144的鉴定及其对DON的影响

doi:10.7668/hbnxb.20191619

摘要/Abstract

摘要： 为研究控制禾谷镰孢菌生长及抑制其脱氧雪腐镰刀菌烯醇（Deoxynivalenol，DON）产生的安全、有效生物防治技术，利用植物内生菌分离技术从小麦发病麦穗中分离得到的256株内生菌，通过平板对峙、共培试验，筛选到1株可显著抑制小麦赤霉病菌生长及降低DON含量的拮抗内生细菌YB-144。通过其形态特征、生化特性和16S rDNA序列比对，结果表明，菌株YB-144为贝莱斯芽孢杆菌，菌株YB-144对禾谷镰孢菌PH-1的抑菌带宽度为13.6 mm，共培养15 h后孢子萌发抑制率高达81.67%，DON含量下降66.6%，DON合成基因Tri5、Tri3、Tri8的相对表达量分别表现出5.0，1.6，1.2倍的下调。同时菌株YB-144对小麦纹枯病菌、小麦根腐病菌等9种植物病原真菌具有显著的抑制作用，该菌株具有较好的开发利用潜力。

关键词: 小麦赤霉病, 禾谷镰孢菌, 贝莱斯芽孢杆菌, 脱氧雪腐镰刀菌烯醇, 内生细菌

Abstract: In order to study the safe and effective biological control technology to control the growth of Fusarium graminearum and inhibition its of production, 256 strains of endophytic bacteria were isolated from surface sterilized spikes of wheat with scab symptoms by plant endophyte isolation technology. Of them,a strain of antagonistic endophytic bacteria YB-144, which could significantly inhibit the growth of Fusarium graminearum and decrease DON content, was screened through the plate confrontation and co culture experiments.Strain YB-144 was identified as Bacillus velezensis based on morphological characteristics, biochemical characteristics and 16S rDNA gene sequence analysis.The inhibitory band width of the strain YB-144 against Fusarium graminearum PH-1 was 13.6 mm. After 15 h of co-culture, the inhibition rate of spore germination was as high as 81.67%, and the content of DON decreased by 66.6%. The expression levels of DON synthetic genes including Tri5, Tri3 and Tri8 were down-regulated by 5.0, 1.6 and 1.2 times, respectively. In addition, strain YB-144 displayed the antagonist effects against other 9 different plant pathogens, such as Rhizoctoniasolani cerealis and Bipolaris sorokiniana et al. The above results demonstrated that strain YB-144 has good potentials of exploitation and utilization.

Key words: Fusarium head blight, Fusarium graminearum, Bacillus velezensis, DON, Endophytic bacteria

中图分类号:

S435.121

张立勇, 夏明聪, 徐文, 王琦, 张洁, 孙润红, 潘娅梅, 陈瑞雪, 吴坤, 杨丽荣. 拮抗小麦赤霉病内生细菌YB-144的鉴定及其对DON的影响[J]. 华北农学报, 2020, 35(6): 172-179. doi: 10.7668/hbnxb.20191619.

ZHANG Liyong, XIA Mingcong, XU Wen, WANG Qi, ZHANG Jie, SUN Runhong, PAN Yamei, CHEN Ruixue, WU Kun, YANG Lirong. Identification of Antagonistic Endophytic Bacterium YB-144 Strain from Fusarium Head Blight Against and Its Effect on DON[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 172-179. doi: 10.7668/hbnxb.20191619.

http://www.hbnxb.net/CN/Y2020/V35/I6/172

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