东北地区小麦赤霉病镰孢菌种群及其致病性测定

doi:10.7668/hbnxb.2015.03.035

摘要/Abstract

摘要： 为了明确东北地区小麦赤霉病镰孢菌的种群组成及分布,分别从辽宁省沈阳市、黑龙江省哈尔滨市和密山市、内蒙古自治区扎兰屯市、呼伦贝尔市和通辽市等地区采集小麦赤霉病病样,经单孢分离纯化共得到118株镰孢菌,传统形态学鉴定的基础上,采用基因组DNA的 EF-1α序列分析技术进行了镰孢菌种类的辅助鉴定,确定属于7个种:禾谷镰孢菌为优势种,分离频率为64.41%,藤仓镰孢菌为次优势种,分离频率为18.64%;燕麦镰孢菌、尖孢镰孢菌、木贼镰孢菌、锐顶镰孢菌和轮枝镰孢菌的分离频率分别为5.08%,3.39%,3.39%,3.39%,1.70%。同时,在玉米成株期进行了致病性测定,结果表明,小麦赤霉病镰孢菌可以侵染玉米,较玉米茎腐镰孢菌的致病力低。

关键词: 小麦赤霉病, 镰孢菌, 种群, 致病性, EF-1α序列分析

Abstract: To determine population structure and distribution of Fusarium species causing head blight of wheat,60 wheat samples showing symptoms of Fusarium head blight from 15 areas 8 cities and counties were collectted in Northeast of China.A total of 118 Fusarium isolates were identified based on morphological characters and modern molecular systematics method.The results showed that 118 Fusarium isolates belong to 7 species, Fusarium graminearum was the most prevalent species,representing 64.41% of the total isolates,followed by Fusarium fujikuroi, representing 18.64%, Fusarium avenaceum, Fusarium oxysporum, Fusarium equiseti, Fusarium acuminatum and Fusarium verticillioides representing 5.08%,3.39%,3.39%,3.39% and 1.70%.At the same time,their pathogenicity were determined in stem of adult-stage maize,the results showed that Fusarium graminearum isolated from wheat can infect maize,but the pathogenicity of Fusarium graminearum causing maize stalk rot were significantly greater than the Fusarium graminearum causing wheat head scab.

Key words: Wheat head blight, Fusarium spp., Population, Pathogenicity, EF-1&alpha, sequence analysis

中图分类号:

S435.121

潘晓静, 陈楠, 姚远, 刘限, 高增贵. 东北地区小麦赤霉病镰孢菌种群及其致病性测定[J]. 华北农学报, 2015, 30(3): 205-210. doi: 10.7668/hbnxb.2015.03.035.

PAN Xiao-jing, CHEN Nan, YAO Yuan, LIU Xian, GAO Zeng-gui. Population and Pathogenicity of Fusarium spp.Causing Wheat Head Blight in Northeast of China[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 205-210. doi: 10.7668/hbnxb.2015.03.035.

http://www.hbnxb.net/CN/Y2015/V30/I3/205

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