抗戊唑醇禾谷镰孢菌对渗透压敏感性及电导率变化研究

doi:10.7668/hbnxb.2011.S2.039

华北农学报 ›› 2011, Vol. 26 ›› Issue (S2): 184-189. doi: 10.7668/hbnxb.2011.S2.039

抗戊唑醇禾谷镰孢菌对渗透压敏感性及电导率变化研究

叶滔^1,2,3, 马志强^1,2, 张小风^1,2, 王文桥^1,2, 韩秀英^1,2, 王志伟⁴

1. 河北省农林科学院植物保护研究所, 河北保定 071000;
2. 河北省农业有害生物综合防治工程技术研究中心, 河北保定071000;
3. 河北农业大学植物保护学院农药系, 河北保定 071001;
4. 河北农业大学农场二分场, 河北保定 071000

收稿日期:2011-10-20 出版日期:2011-12-31
通讯作者: 马志强(1959-),男,河北保定人,研究员,主要从事杀菌剂毒理学与有害生物抗药性研究.
作者简介:叶滔(1984-),男,湖北崇阳人,在读硕士,主要从事杀菌剂毒理学与有害生物抗药性研究.
基金资助:
国家公益性行业(农业)科研专项项目(nyhyzx200803015;nyhyzx201203035)

Study on the Changes of Relative Conductivity and Osmolarity Sensitivity to Tebuconazole-resistant Isolates of Fusarium graminearum

YE Tao^1,2,3, MA Zhi-qiang^1,2, ZHANG Xiao-feng^1,2, WANG Wen-qiao^1,2, HAN Xiu-ying^1,2, WANG Zhi-wei⁴

1. Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences, Baoding 071000, China;
2. IPM Centre of Hebei Province, Baoding 71000, China;
3. Department of Pesticide Science, College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China;
4. The Second Sessions of the Farm, Agricultural University of Hebei, Baoding 071001, China

Received:2011-10-20 Published:2011-12-31

摘要/Abstract

摘要： 通过紫外诱导和药剂驯化获取禾谷镰孢菌的抗戊唑醇菌株,探索了抗戊唑醇禾谷镰孢菌的抗药性机制。抗药菌株经转接培养17代后筛选遗传稳定性抗性菌株,利用渗透压法和电导率法测定抗药菌株与亲本菌株之间的生理性差异。所获得的9个抗性菌株,抗性水平最大值为186.03,最小值为34.26。其中,有5个抗性菌株能保持较高的抗性水平。在含2.0%和4.0%的浓度葡萄糖培养基下,抗性菌株的菌落生长直径要比亲本菌株大,而含相同浓度的KCl培养基中,抗性菌株与敏感菌株的菌落生长变化无规律。在不同时间下,抗性菌株和亲本菌株的电导率变化明显,药剂驯化抗性菌株电导率变化比敏感菌株大,经200 min后药剂驯化的抗性菌株比紫外诱导的抗性菌株电导率普遍高。禾谷镰孢菌抗戊唑醇的机制可能与细胞膜通透性的改变和短时间内快速排泄杀菌剂有关。

关键词: 戊唑醇, 禾谷镰孢菌, 渗透压, 电导率

Abstract: The aim of the study is to obtain the resistant isolates of Fusarium graminearum Schw by ultraviolet-induction or fungicide-treated myceliums.With the physiology characteristics compared between the sensitive and resistant isolates,we can study on resistance mechanism of Fusarium graminearum to tebuconazole.The resistant isolates were obtained through ultraviolet-induction and tebuconazole-treated myceliums,some isolates could keep stablely resistant level during the period of 17 subcultures.By studying on osmolarity and conductivity,the physiology characteristics of the resistant and sensitive isolates were compared.The results showed that nine resistant mutants were obtained,the most resistance ratio reached to 180.06,the lowest resistance ratio showed 34.26,and five resistant isolates could be keeping high resistance level.In the concentrations of 2.0% and 4.0% dextrose,the colony growth diameter of resistant isolates was bigger than that of their parental isolates.But the colony growth diameter of resistant isolates and their parental isolates showed no regular in the same concentrations of KCl.In different times,the changes of conductivity to resistant isolates and parental isolates at different concentrations of tebuconazole were obvious,and the changes of conductivity to tebuconazole-treated resistant isolates was bigger than parental isolates,and the relative leakage of resistant isolates which were obtained by tebuconazole-treated was common higher than that of resistant isolates by ultraviolet-induction after 200 min.Resistance mechanism of F.graminearum to tebuconazole might be concern with the change of cell membrane permeability and excrete quickly fungicide in short time.

Key words: Tebuconazole, F.graminearum, Osmoric potential, Relative conductivity

中图分类号:

S435.111

叶滔, 马志强, 张小风, 王文桥, 韩秀英, 王志伟. 抗戊唑醇禾谷镰孢菌对渗透压敏感性及电导率变化研究[J]. 华北农学报, 2011, 26(S2): 184-189. doi: 10.7668/hbnxb.2011.S2.039.

YE Tao, MA Zhi-qiang, ZHANG Xiao-feng, WANG Wen-qiao, HAN Xiu-ying, WANG Zhi-wei. Study on the Changes of Relative Conductivity and Osmolarity Sensitivity to Tebuconazole-resistant Isolates of Fusarium graminearum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S2): 184-189. doi: 10.7668/hbnxb.2011.S2.039.

http://www.hbnxb.net/CN/Y2011/V26/IS2/184

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抗戊唑醇禾谷镰孢菌对渗透压敏感性及电导率变化研究

Study on the Changes of Relative Conductivity and Osmolarity Sensitivity to Tebuconazole-resistant Isolates of Fusarium graminearum

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抗戊唑醇禾谷镰孢菌对渗透压敏感性及电导率变化研究

Study on the Changes of Relative Conductivity and Osmolarity Sensitivity to Tebuconazole-resistant Isolates of Fusarium graminearum

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