山东省小麦赤霉病菌的种群组成及毒素化学型分析

doi:10.7668/hbnxb.2017.S1.007

华北农学报 ›› 2017, Vol. 32 ›› Issue (S1): 37-42. doi: 10.7668/hbnxb.2017.S1.007

所属专题： 小麦；

山东省小麦赤霉病菌的种群组成及毒素化学型分析

高先悦¹, 高淑敏¹, 刘少杰², 张莉¹, 于金凤¹

1. 山东农业大学植保学院植物病理学系, 山东泰安 271018;
2. 山东省烟台农业学校, 山东烟台 265100

收稿日期:2017-09-16 出版日期:2017-12-28
通讯作者: 于金凤(1965-),女,山东海阳人,教授,博士,博士生导师,主要从事真菌与真菌资源利用研究。
作者简介:高先悦(1990-),男,山东蓬莱人,在读硕士,主要从事真菌与真菌资源利用研究。
基金资助:
国家重点研发计划课题（2017YFD0201705）；山东省现代农业产业技术体系创新团队建设专项资金（SDAIT-01-09）

Analysis on the Species Composition and Mycotoxin Chemotypes of Wheat Fusarium Head Blight in Shandong Province

GAO Xianyue¹, GAO Shumin¹, LIU Shaojie², ZHANG Li¹, YU Jinfeng¹

1. Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China;
2. Shandong Yantai Agricultural School, Yantai 265100

Received:2017-09-16 Published:2017-12-28

摘要/Abstract

摘要： 为了明确其在山东的种群组成及毒素类型，在2014，2015年从山东11个地区的28县、市共采集小麦病穗标本300余份，采用常规的组织分离方法，得到小麦赤霉病菌菌株338株。根据采集地点随机选取120株作为测试菌株，采用鉴定种和鉴定B型毒素化学型的特异性引物对选取菌株进行鉴定。结果表明，120个供试菌株均为禾谷镰孢菌进化宗系群，分为两种进化类型：禾谷镰孢菌和亚洲镰孢菌，分别为108株和12株，其中禾谷镰孢菌占90%，是优势致病型。亚洲镰孢菌是长江中下游麦区的优势种，此类型是首次在山东境内被检测到，且其在山东的分布与前茬作物种类无明显的相关性。毒素化学型鉴定结果表明，供试的120个菌株中有118株属脱氧雪腐镰孢菌烯醇（DON）毒素化学型，仅有2个菌株产生雪腐镰孢菌烯醇（NIV）毒素。其中，DON毒素化学型菌株又进一步划分为15-乙酰脱氧雪腐镰孢菌烯醇（15-AcDON）和3-乙酰脱氧雪腐镰孢菌烯醇（3-AcDON）。其对应菌株分别为101株和17株。

关键词: 小麦赤霉病, 种群组成, 毒素化学型, 前茬作物

Abstract: In order to determine the species composition and toxin chemical type of FHB in Shandong,during 2014 to 2015 from 28 counties and cities in Shandong,collected more than 300 samples of FHB,isolated 338 strains.According to the collected location select 120 strains randomly as the tested strains,specific primers were used for identification strain and identification of type B toxin chemical type.The results showed that 120 strains of Fusarium graminearum lineage clade, divided into two species:F.graminearum and F.asiaticum,respectively 108 and 12 strains,of which F.graminearum accounted for 90% was the predominant. F.asiaticum was the advantage species in the Yangtze River area,and this evolutionary type was for the first time detected in Shandong,and its distribution had no obvious relationship with the previous crop in Shandong.The toxin chemical analysis showed that there are 118 strains had deoxynivalenol (DON) chemotypes,only two strains produced nivalenol (NIV) chemotypes.The 118 DON strains was further divided into 15-acetyldeoxynivalenol (15-AcDON) and 3-acetyldeoxynivalenol (3-AcDON),the corresponding strains were 101 strains and 17 strains.

Key words: Fusarium head blight, Population structure, Mycotoxin chemotypes, Preceding crop

中图分类号:

S432.1

高先悦, 高淑敏, 刘少杰, 张莉, 于金凤. 山东省小麦赤霉病菌的种群组成及毒素化学型分析[J]. 华北农学报, 2017, 32(S1): 37-42. doi: 10.7668/hbnxb.2017.S1.007.

GAO Xianyue, GAO Shumin, LIU Shaojie, ZHANG Li, YU Jinfeng. Analysis on the Species Composition and Mycotoxin Chemotypes of Wheat Fusarium Head Blight in Shandong Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(S1): 37-42. doi: 10.7668/hbnxb.2017.S1.007.

http://www.hbnxb.net/CN/Y2017/V32/IS1/37

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