摘要： 为了解猪博卡病毒在不同年龄健康猪群中的感染情况，通过对GenBank登录的猪博卡病毒基因序列分析设计2对引物可分别扩增PBoV1/2的部分NS1基因和PBoV3/4/5的部分VP1基因。提取2016年8-12月收集自湖南5个集约化猪场2~20周龄猪共430份肛门拭子样品的DNA并进行PCR检测。PBoV阳性率为64.6%（278/430），PBoV1/2阳性率为27.2%（117/430），PBoV3/4/5阳性率为45.1%（194/430），混合感染率为7.7%（33/430），PBoV3/4/5阳性率显著高于PBoV1/2阳性率（P=0.034）。PBoV1/2在2~10周检出率高于其他周龄，但是与其他周龄猪体内检出率统计分析无显著性差异，PBoV3/4/5主要感染8~16周猪，与4周以内猪差异显著，与其他猪群差异不显著。对各型的核苷酸与参考序列核苷酸相似性分析显示PBoV1/2之间的平均相似度为85.4%，PBoV3/4/5之间的平均相似度为67.2%。PBoV在健康猪群中流行广泛且存在共同感染现象，对猪的健康尤其是肠道微生物平衡可能存在潜在影响。

关键词: 猪博卡病毒, 流行性, 进化分析, 混合感染, 致病性

Abstract: To evaluate the prevalence of PBoV in healthy pig in different age,primers targeting to partial NS1 gene of PBoV1/2 and VP1 gene of PBoV3/4/5 were designed based on the analysis of PBoV sequences from GenBank.Total 430 anal swab species were collected from 5 pig farms with the pig'age of 2-20 weeks from August to December 2016 in Hunan Province.The total positive rate of PBoV was 64.6% (278/430),PBoV1/2 for 27.2%(117/430),PBoV3/4/5 for 45.1%(194/430) and with 7.7%(33/430) positive rate of coinfection.The mainly infection age of PBoV1/2 ranged 2-10 weeks,and PBoV3/4/5 from 8-16 weeks.The average nucleotide identity of obtained sequences and references of PBoV1/2 and PBoV3/4/5 was 85.4% and 67.2%,respectively.High prevalence and coinfection were observed in clinically healthy pigs,which may cause potential effect to the microbes in the gut.

Key words: PBoV, Prevalence, Phylogenetic analysis, Coinfection, Pathogenicity

中图分类号: 

• S855.3