摘要： 为了分析河南省猪流行性腹泻病毒（PEDV）毒株S和ORF3基因变异情况，于2015年1-12月收集河南省规模化猪场的150份PED疑似病料利用RT-PCR方法进行S和ORF3基因检测，共扩增到15株PEDV的S和ORF3基因，回收纯化PCR产物克隆至T载体，并进行序列分析，结果显示，15株S基因与CV777相比，核苷酸同源性为93.6%～93.9%，氨基酸同源性为92.2%～93.1%，而且存在不同碱基插入和缺失；ORF3基因与CV777相比，核苷酸同源性为97.7%～100%，氨基酸同源性为93.7%～100%，与疫苗毒株相比，不存在氨基酸的缺失现象。进化树分析基于S基因扩增的15株独立成群，与其他参考毒株亲缘较远；基于ORF3基因扩增的15个毒株与国内分离株、美国株及韩国株均有较近的亲缘关系，与经典毒株CV777株亲缘关系较远。对于S和ORF3基因在整个进化关系中，试验中的15个河南株均相对独立成群，与经典毒株CV777及国内所使用的疫苗株，亲缘关系较远，15株河南株的S和ORF3基因存在基因变异，为河南省PED的分子流行病学研究和防控提供技术支持。

关键词: 河南省, 猪流行性腹泻病毒, S和ORF3基因, 序列分析

Abstract: In order to analyze the S and ORF3 genes of PEDV in Henan Province, 150 PED suspected diseases were isolated from different pig farms in Henan Province from January to December, 2015, and the sequences of S and ORF3 genes were analyzed. The result showed that the nucleotide and amino acid homologies of S genes from 15 strains with CV777 were 93.6%-93.9% and 92.2%-93.1%, respectively. And some base insertions and deletions were detected. Compared with CV777, the nucleotide homology of ORF3 gene was 97.7%-100% and the amino acid homology was 93.7%-100%. Additionally, there was no base insertion and deletion compared with the vaccine strain. The Phylogenetic tree was based on the 15 PEDV of S gene amplified and the relationship of these strains were relatively distant from other reference strains. The relationships of the 15 strains, amplified based on the ORF3 gene, were closely related to domestic isolates, American strains and Korean strains. For the entire evolutionary relationship of S and ORF3 genes, 15 Henan strains in this experiment were relatively independent and their relationships with classic strain CV777 and other vaccine strains used in China were distant.The experiment analyzed of the S and ORF3 gene sequence features in prevailing of Henan region, which provided a support of molecular epidemiology study and prevention and control of PED in Henan Province.

Key words: Henan Province, Porcine epidemic diarrhea virus, S and ORF3 gene, Sequence analysis

中图分类号: 

• Q78