北京地区侵染菊花的番茄斑萎病毒分子检测与基因组部分序列分析

doi:10.7668/hbnxb.20190379

摘要/Abstract

摘要： 为了确定北京菊花产区是否发生了番茄斑萎病毒病，了解该病毒北京分离物的序列变异情况，对该地区发生的TSWV进行了分子检测，并对该病毒编码的依赖RNA的RNA聚合酶（RDRP）、多糖蛋白（Gn-Gc）、运动蛋白（NSm）、外壳蛋白（N）、非结构蛋白（NSs）等5个基因进行了序列分析。结果表明，TSWV北京地区菊花分离物（Beijing-jh）的5个编码基因与国内各分离物的进化关系较远，与来自韩国和西班牙的分离物亲缘关系较近。相似性分析表明， N基因是最为保守的基因，核酸平均相似性为97.2%，蛋白为98.6%，RDRP的变异性最大，核酸平均相似性为95.4%，蛋白为96.8%，且Beijing-jh分离物的5个基因编码的氨基酸与来自西班牙分离物的相似性最大，初步表明，该分离物可能是由于贸易活动由国外传入我国。

关键词: 菊花, 番茄斑萎病毒, 分子检测, 序列分析

Abstract: In order to determine the occurrence and variation of Tomato spotted wilt virus (TSWV)in chrysanthemum in Beijing,we collected suspected chrysanthemum plants and performed molecular detection and partial genome sequence analysis. Sequences of RNA-dependent RNA polymerase(RDRP),glycoprotein(Gn-Gc),movement protein(NSm),non-structural protein(NSs),and coat protein(N)encoded by the virus were determined. Phylogenetic analysis showed that the isolate named Beijing-jh was most closely related to TSWV isolates of Spain and South Korea and was far related to TSWV isolates from China. The similarity analysis showed that the N gene was the most conservative gene. The average similarity of nucleic acids was 97.2%,the protein was 98.6%. The RDRP had the greatest variability,the average similarity of nucleic acids was 95.4% and the protein was 96.8%. The amino acids of the five proteins of TSWV from Beijing-jh isolate were the most similar to those from Spain. It is initially shown that Beijing-jh isolate was imported through international trade.

Key words: Chrysanthemum, Tomato spotted wilt virus, Molecular detection, Sequences analysis

中图分类号:

S436.8
Q78

金凤媚, 宋建, 薛俊, 陈东亮, 王姝, 张越, 孙海波. 北京地区侵染菊花的番茄斑萎病毒分子检测与基因组部分序列分析[J]. 华北农学报, 2020, 35(1): 175-184. doi: 10.7668/hbnxb.20190379.

JIN Fengmei, SONG Jian, XUE Jun, CHEN Dongliang, WANG Shu, ZHANG Yue, SUN Haibo. Molecular Detection and Partial Genome Sequence Analysis of Tomato spotted wilt virus Infected Chrysanthemum in Beijing[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 175-184. doi: 10.7668/hbnxb.20190379.

http://www.hbnxb.net/CN/Y2020/V35/I1/175

参考文献

[1] Parrella G,Gognalons P,Gebre-Selassiè K,Vovlas C,Marchoux G. An update of the hostrange of Tomatos potted wilt virus[J]. Journal of Plant Pathology,2003,85(4):227-264.
[2] Scholthof K B G,Adkins S,Czosnek H,Palukaitis P,Jacquot E,Hohn T,Hohn B,Saunders K,Candresse T,Ahlquist P,Hemenway C,Foster G D. Top10 plant viruses in molecular plantpathology[J].Molecular Plant Pathology,2011,12(9):938-954.doi:10.1111/j.1364-3703.2011.00752.
x.
[3] 苏大昆,袁宣泽,谢永红,王淑荣,丁海.成都、渡口两市番茄中检出番茄斑萎病毒[J].植物病理学报,1987(4):255-256.
Su D K,Yuan X Z,Xie Y H,Wang S R,Ding H. Tomato spotted wilt virus in tomato in Chengdu and Dukou[J].Acta Phytopathologica Sinica,1987(4):255-256.
[4] 许泽永,张宗义,陈金香.番茄斑萎病毒(TSWV)广州分离物生物学特性研究[J].植物病理学报,1989(4):198.
Xu Z Y,Zhang Z Y,Chen J X. Biological properties of a Guangdong isolate of Tomato spotted wilt virus (TSWV)[J]. Acta Phytopathologica Sinica,1989(4):198.
[5] 姚革.四川晒烟上发现番茄斑萎病毒(TSWV)[J].烟草科技,1992(4):2-4.doi:10.13496/j.issn.1007-5119.1992.04.001.
Yao G. Tomato spotted wilt virus was identified in tobacco in Sichuan[J]. Tobacco Science & Technology,1992(4):2-4.
[6] 李飞,吴青君,徐宝云,谢文,王少丽,张友军.北京地区发现番茄斑萎病毒[J].植物保护,2012,38(6):186-188,191.doi:10.3969/j.issn.0529-1542.2012.06.043.
Li F,Wu Q J,Xu B Y,Xie W,Wang S L,Zhang Y J. Tomato spotted wilt virus was identified in Beijing[J]. Plant Protection,2012,38(6):186-188,191.
[7] Sun X H,Gao L L,Wang S L,Wang C L,Yang Y Y, Wang X Y, Zhu X P. First report of Tomato spotted wilt virus infecting pumpkinin China[J]. Journal of Plant Pathology,2016,98(3):677-697.
[8] Li Y Y,Xiao L,Tan G L,Fu X P,Li R H,Li F.First report of Tomato spotted wilt virus on celery in China[J].Plant Disease,2015,99(5):734.doi:10.1094/PDIS-11-14-1105-PDN.
[9] Xiao L,Li Y Y,Lan P X,Tan G L,Ding M,Li F.First repor of Tomato spotted wilt virus infecting cowpea in China[J].Plant Disease,2016,100(1):233.doi:10.1094/PDIS-04-15-0495-PDN.
[10] 曹金强,谢学文,柴阿丽,李宝聚.番茄斑萎病毒病在宁夏的发现与防治[J].中国蔬菜,2016(4):87-89.doi:10.3969/j.issn.1000-6346.2016.04.028.
Cao J Q,Xie X W,Chai A L,Li B J. Identification and control of Tomato spotted wilt virus in Ningxia[J]. China Vegetables,2016(4):87-89.
[11] 李丞,李永忠,文国松,刘雅婷.烟草番茄斑萎病的研究进展[J].中国农业科学,2007,23(2):374-378.doi:10.3969/j.issn.1000-6850.2007.02.089.
Li C,Li Y Z,Wen G S,Liu Y T. Advance of research on tobacco tomato spotted wilt disease[J]. Chinese Agricultural Science Bulletin,2007,23(2):374-378.
[12] Chen D L,Lou C,Liu H,Li X M,Cheng X,Li M Y,Wang L L,Huang C L. First report of Chrysnthemum stem blight and dieback caused by Tomato spotted wilt virus in China[J]. Plant Disease,2018,102(5):1047-1048.doi:10.1094/PDIS-07-17-1088-PDN.
[13] 李明远,陈东亮,李雪梅,王玲玲,黄丛林. 李明远断病手迹(八十六)北京蔬菜上的几种番茄斑萎病毒[J].农业工程技术,2018,38(7):59-61.
Li M Y,Chen D L,Li X M,Wang L L,Huang C L. Limingyuan' hand mark(86)several kinds of Tomato spotted wilt virus on Beijing vegetables[J]. Agricultural Engineering Technology,2018,38(7):59-61.
[14] Vaira A M,Semeria L,Crespi S,Lisa V,Allavena A,Accotto G P.Resistance to tospoviruses in Nicotiana benthamiana transformer with the N gene of Tomato spotted wilt virus:correlation between transgene expression and protection in primary transformants[J]. Molecular Plant-microbe Interactions,1995,8(1):66-73.doi:10.1094/MPMI-8-0066.
[15] 唐前君,孙书娥,刘勇,谭新球,肖启明,谢丙炎,张德咏.番茄斑萎病毒核衣壳蛋白基因的克隆与分析[J].植物保护,2010,36(5):65-69.doi:10.3969/j.issn.0529-1542.2010.05.014.
Tang Q J,Sun S E, Liu Y,Tan X Q,Xiao Q M,Xie B Y,Zhang D Y. Cloning and sequence analysis of the nucleoprotein genes of Tomato spotted wilt virus isolates from Yunnan[J].Plant Protection,2010,36(5):65-69.
[16] 金凤媚,薛俊,宋建,于海涛,段红英.天津地区番茄褪绿病毒的分子检测与基因组部分序列分析[J]. 华北农学报,2016,31(2):23-27.doi:10.7668/hbnxb.2016.02.005.
Jin F M,Xue J, Song J,Yu H T,Duan H Y. Molecular detection and partial genome sequence analysis of Tomato chlorosis virus in Tianjin[J]. Acta Agriculturae Boreali-Sinica,2016,31(2):23-27.
[17] 李洁,迟胜起,杨勤民,丁天波,褚栋. 山东烟台地区发生番茄斑萎病毒病危害[J].植物保护,2017,43(1):228-232,236.doi:10.3969/j.issn.0529-1542.2017.01.043.
Li J,Chi S Q,Yang Q M,Ding T B,Chu D. Identification of Tomato spotted wilt virus disease in Shandong,China[J]. Plant Protection,2017,43(1):228-232,236.
[18] 蒙姣荣,赵家新,蔡琦,邹承武,李战彪,廖咏梅,陈保善.广西烟草病毒病发生情况调查和病原病毒的鉴定[J].广西植保,2012,25(4):1-2.
Meng J R,Zhao J X,Cai Q,Zou C W,Li Z B,Liao Y M,Chen B S. The investigation of virus affecting occurrence and pathogen identification in tobacco in Guangxi[J]. Guangxi Plant Protection,2012,25(4):1-2.
[19] 孙淼,荆陈沉,楚成茹,吴根土,孙现超,谢艳,刘勇,青玲.重庆辣椒上番茄斑萎病毒的血清学检测及分子鉴定[J].园艺学报,2017,44(3):487-494.doi:10.16420/j.issn.0513-353x.2016-0733.
Sun M,Jing C C,Chu C R,Wu G T,Sun X C,Xie Y,Liu Y,Qing L.Serological detection and molecular identification of Tomato spotted wilt virus in Pepper in Chongqing[J].Acta Horticulturae Sinica,2017,44(3):487-494.
[20] French J M,Goldberg N P,Randall J J,Hanson S F. New Mexico and the southwestern US are affected by a unique population of Tomato spotted wilt virus (TSWV)strains[J]. Archives of Virology,2016,161(4):993-998.doi:10.1007/s00705-015-2707-5.
[21] 杨立,刘向军.北京地区一种侵染菊花的线条病毒[J].植物病理学报,1995,25(1):57-60.
Yang L,Liu X J. Identification of a Filamentous virus infecting Chrysnthemum[J]. Acta Phytopathologica Sinica,1995,25(1):57-60.
[22] 李明远,陈东亮,李雪梅,姜明元.李明远断病手迹(八十一)菊花两种类病毒的鉴定[J].农业工程技术,2017,37(16):62-64.doi:10.16815/j.cnki.11-5436/s.2017.16.013.
Li M Y,Chen D L,Li X M,Jiang M Y. Identification of two kinds of virus in Chrysanthemum:Limingyuan' hand mark(81)[J]. Agricultural Engineering Technology,2017,37(16):62-64.
[23] 杨华,崔无玕,张升,孙晓军. 外来入侵害虫——西花蓟马在新疆的发生为害[J].新疆农业科学,2010,47(11):2252-2253.
Yang H,Cui W G,Zhang S,Sun X J. The occurrence and damage of the exotic invasive pest:western flower thrip(Frankliniella occidentalis)in Xinjiang[J]. Xinjiang Agricultural Sciences,2010,47(11):2252-2253.
[24] 吕要斌,张治军,吴青君,杜予州,张宏瑞,于毅,王恩东,王鸣华,王满囷,童晓立,吕利华,谭新球,付卫东. 外来入侵害虫西花蓟马防控技术研究与示范[J].应用昆虫学报,2011,48(3):488-496.
Lü Y B,Zhang Z J,Wu Q J,Du Y Z,Zhang H R,Yu Y,Wang E D,Wang M H,Wang M H,Tong X L,Lü L H,Tan X Q,Fu W D. Research progress of the monitoring,forecast and sustainable management of invasive alien pest Frankliniella occidentalis in China[J]. Chinese Journal of Applied Entomology,2011,48(3):488-496.

选择文件类型/文献管理软件名称

选择包含的内容