华北农学报 ›› 2015, Vol. 30 ›› Issue (S1): 235-240. doi: 10.7668/hbnxb.2015.S1.042

所属专题: 西瓜

• 论文 • 上一篇    下一篇

利用生理生化参数评价麝香草酚对西瓜种子萌发的影响

薛延丰1,2,3,4, 李敏1,5, 陈健1,2,3,4, 张猛1,2,3,4, 石志琦1,2,3,4   

  1. 1. 江苏省农业科学院 食品质量安全检测研究所, 江苏 南京 210014;
    2. 江苏省食品质量安全重点实验室-省部共建国家 重点实验室培育基地, 江苏 南京 210014;
    3. 农业部农产品质量安全控制技术与标准重点实验室, 江苏 南京 210014;
    4. 农业部农产品质量安全风险评估实验室, 江苏 南京 210014;
    5. 南京农业大学 植物保护学院, 江苏 南京 210095
  • 收稿日期:2015-10-16 出版日期:2015-12-28
  • 作者简介:薛延丰(1978-),男,河南孟州人,副研究员,博士,主要从事产地环境与食物链安全研究.
  • 基金资助:
    江苏省农业科技自主创新资金项目(CX(14)4079)

Effect of Assessing the Feasibility of Using Physiological and Biochemical Parameters to Assess Thymol for Watermelon Seeds to Improve Germination Characteristic

XUE Yan-feng1,2,3,4, LI Min1,5, CHEN Jian1,2,3,4, ZHANG Meng1,2,3,4, SHI Zhi-qi1,2,3,4   

  1. 1. Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
    2. Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing 210014, China;
    3. Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture, Nanjing 210014, China;
    4. Key Lab of Agro-product Safety Risk Evaluation, Ministry of Agriculture, Nanjing 210014, China;
    5. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2015-10-16 Published:2015-12-28

摘要: 通过研究麝香草酚对西瓜种子进行浸种处理,来判断麝香草酚对西瓜种子的安全性,为该产品在田间预防西瓜枯萎病提供理论依据.结果表明,麝香草酚浓度小于300 mg/L时,浸种后对种子的发芽势、发芽率、发芽指数和活力指数与对照相比没有显著影响,但随着处理浓度的增加,发芽势、发芽率、发芽指数和活力指数与对照相比显著降低;麝香草酚对西瓜生长和叶绿素含量的影响与种子萌发的影响相似,当浓度小于300 mg/L时,对西瓜生长和叶绿素含量的影响与对照相比差异不显著(鲜质量除外);麝香草酚对西瓜根系活力的影响与对照相比呈现出逐渐减低的趋势,低浓度(100 mg/L)处理下,西瓜根系活力最大,与对照差异显著,200 mg/L时,根系活力有所增加,但与对照差异不显著,随着处理浓度的增加,根系活力与对照相比显著降低;当麝香草酚浓度小于300 mg/L时,SOD活性均显著大于对照,但随着处理浓度的增加,SOD活性显著降低;POD和CAT的变化趋势相似,在100,200 mg/L处理下,POD和CAT活性有所增加,但与对照差异不显著;从麝香草酚处理浓度与种子萌发及生理生化参数变化的相关性分析,种子萌发及生理生化参数与高浓度麝香草酚呈负相关关系.说明当麝香草酚处理浓度小于300 mg/L时,用麝香草酚处理西瓜种子预防西瓜枯萎病不会影响西瓜萌发及生长.

关键词: 麝香草酚, 西瓜枯萎病, 植物源杀菌剂, 西瓜

Abstract: The seeds and seedlings of watermelon were handled by the various concentrations of thymol in order to assess the safety of germination and growth thymol on watermelon seed.There will be provide the theoretical basis for the product used to prevent the watermelon wilt disease.The results showed that the changes of germination energy,germination percentage,germination index and vigor index were no significant difference less than 300 mg/L thymol concentration,whereas it markedly decreased with the rising of the thymol concentration.The effects of thymol on the growth and chlorophyll content were same to the germination.When the thymol concentration was less than 300 mg/L,the growth and chlorophyll content were no significant difference compared with the control (except for the fresh weight).Under the treatment of thymol soaked seeds the root activity gradually reduced with the increase of thymol concentration.The root activity significantly increased compared to the control in 100 mg/L thymol concentration.In 200 mg/L thymol concentration the root activity was higher than the control,whereas there was no significant difference compared to the control.The root activity decreased with the rising of the thymol concentration.The SOD activity significantly increased less than 300 mg/L thymol concentration,whereas it markedly decreased with the rising of the thymol concentration.The POD activity had the same trend as the CAT activity.In 100,200 mg/L thymol concentration the POD and CAT activities were higher than the control,whereas there was no significant difference compared to the control.From the correlations analysis of seed germination parameters and physiological and biochemical indexes with the thymol concentrations knew that it was a negative correlation relationship between seed germination parameters and physiological and biochemical indexes with the higher thymol concentration.All the results showed that the germination and growth were not affected less than 300 mg/L thymol concentration being used to prevent the watermelon wilt disease.

Key words: Thymol, Watermelon wilt disease, Botanical Fungicides, Watermelon

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引用本文

薛延丰, 李敏, 陈健, 张猛, 石志琦. 利用生理生化参数评价麝香草酚对西瓜种子萌发的影响[J]. 华北农学报, 2015, 30(S1): 235-240. doi: 10.7668/hbnxb.2015.S1.042.

XUE Yan-feng, LI Min, CHEN Jian, ZHANG Meng, SHI Zhi-qi. Effect of Assessing the Feasibility of Using Physiological and Biochemical Parameters to Assess Thymol for Watermelon Seeds to Improve Germination Characteristic[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 235-240. doi: 10.7668/hbnxb.2015.S1.042.

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