小型西瓜核心种质构建

doi:10.7668/hbnxb.2015.S1.011

华北农学报 ›› 2015, Vol. 30 ›› Issue (S1): 61-65. doi: 10.7668/hbnxb.2015.S1.011

所属专题： 西瓜；

小型西瓜核心种质构建

刘子记¹, 朱婕², 党选民¹, 詹园凤¹

1. 中国热带农业科学院热带作物品种资源研究所, 农业部华南作物基因资源与种质创制重点开放实验室, 海南儋州 571737;
2. 海南大学园艺园林学院, 海南海口 570228

收稿日期:2015-10-27 出版日期:2015-12-28
通讯作者: 詹园凤(1974-),女,江西南昌人,副研究员,硕士,主要从事蔬菜遗传育种研究.
作者简介:刘子记(1982-),男,山东菏泽人,副研究员,博士,主要从事蔬菜分子生物学及遗传育种研究.
基金资助:
中国热带农业科学院院本级基本科研业务费专项资金(1630032015003)

Study on the Construction of Core Collection in Mini-watermelon

LIU Zi-ji¹, ZHU Jie², DANG Xuan-min¹, ZHAN Yuan-feng¹

1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Danzhou 571737, China;
2. College of Horticulture and Landscape Architecture, Hainan University, Haikou 570228, China

Received:2015-10-27 Published:2015-12-28

摘要/Abstract

摘要： 丰富的种质资源为作物育种和遗传研究提供了广阔的遗传基础,然而近年来急剧增加的种质资源数量给种质资源的保存、研究与利用带来了很大困难.构建核心种质为种质资源的研究和利用提供了便利条件.以410份小型西瓜种质资源为试验材料,基于6个果实性状数据,采用混合线性模型分析方法无偏地预测基因型值,计算种质间的遗传距离,采用不加权类平均法进行聚类分析,按照20%的抽样比率构建小型西瓜核心种质库.采用均值、方差、极差和变异系数4个指标评价核心种质库的优劣.结果表明,马氏距离优于欧氏距离,偏离度取样法优于优先取样法,基于马氏距离和偏离度抽样方法获取的82份核心资源能够代表原群体的遗传多样性.为小型西瓜种质资源的高效利用提供了重要理论依据.

关键词: 小型西瓜, 核心种质, 遗传距离, 抽样方法

Abstract: Abundant genetic resources could provide broad genetic foundation for plant breeding and genetic research.However,the drastic increase of genetic resources in recent years brought great difficulties for the preservation and utilization of germplasm.The construction of core collection has facilitated the research and utilization of genetic resources.410 mini-watermelon germplasm were used to construct core collection based on 6 fruit traits.The genotypic values were predicted by mixed linear model.The genetic distances among germplasm were calculated based on genotypic values.The unweighted pair-group average method was used for clustering analysis.The core collections of mini-watermelon were constructed with 20% sampling proportion.The genetic variations among core collections were evaluated through comparing mean,variance,range and coefficient of variation.The results showed that the Mahalanobis distance and deviation sampling were better than the Euclidean distance and preferred sampling,respectively.82 core resources acquired based on the Mahalanobis distance and deviation sampling could preserve the genetic diversity of the initial collection,which could provide important theoretical basis for the efficient utilization of mini-watermelon germplasm.

Key words: Mini-watermelon, Core collection, Genetic distance, Sampling strategies

中图分类号:

S651.03

刘子记, 朱婕, 党选民, 詹园凤. 小型西瓜核心种质构建[J]. 华北农学报, 2015, 30(S1): 61-65. doi: 10.7668/hbnxb.2015.S1.011.

LIU Zi-ji, ZHU Jie, DANG Xuan-min, ZHAN Yuan-feng. Study on the Construction of Core Collection in Mini-watermelon[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 61-65. doi: 10.7668/hbnxb.2015.S1.011.

http://www.hbnxb.net/CN/Y2015/V30/IS1/61

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