摘要： 为实现对甜瓜品种快速准确鉴定,利用SSR分子标记技术对6个甜瓜品种及其亲本共18份材料构建指纹图谱.通过利用50对多态性好的甜瓜SSR引物,经扩增电泳分析得到C17、C21、C30共3对引物,可用于构建18份材料的指纹图谱,实现了对这些材料的分子鉴定.另外利用C21引物对材料M324的杂种品种进行纯度鉴定,结果纯度为89%,与田间性状统计结果90%相符.

关键词: 甜瓜, SSR标记, 指纹图谱, 纯度鉴定

Abstract: For the purpose of melon identification,SSR technology was used to establish fingerprint for 18 melon accessions included six F₁ hybrids and their parents.Based on 50 SSR primer pairs were assessed validation of the amplification in above melon accessions.Used three primer pairs C17,C21and C30,SSR fingerprint was obtained for six melon F₁ hybrids and their parents,which was able to distinguish all of the 18 accessions.Moreover,primer C21was applied in purity identification of M324 hybrid,the result indicated that the purity was 89%,which were approximate to their field purity(90%).

Key words: Melon, SSR marker, Fingerprint, Purity identification

中图分类号: 

• S652.03