甜瓜幼苗不同叶位SPAD值与叶绿素含量的变化规律及相关性

doi:10.7668/hbnxb.20190255

摘要/Abstract

摘要： 甜瓜两叶一心期是幼苗植株对低温弱光敏感时期。明确该时期甜瓜不同叶位SPAD值与叶绿素含量的变化规律与相关性，可以为SPAD值无损预测甜瓜叶绿素含量、鉴定甜瓜耐低温弱光特性科学选择测量叶。以甜瓜自交系P148两叶一心期幼苗为试材，测量自下而上3片新叶（Y1、Y2和Y3） SPAD值和叶绿素含量，利用SPSS 13.0软件One-Way-ANOVA程序和Correlation程序，分析甜瓜两叶一心期3片新叶叶绿素a、b和a+b含量的变化规律和与SPAD值的相关性；最后使用Regression程序，选择SPAD值与叶绿素含量相关系数最大的叶位，建立SPAD值与叶绿素含量回归方程，以此叶位作为甜瓜两叶一心时期利用SPAD值预测叶绿素含量的测量叶。结果表明，甜瓜两叶一心期叶片SPAD值和叶绿素含量随着叶位升高而增加，Y3的叶绿素含量极显著地高于Y1和Y2；3个叶位叶绿素含量与SPAD的相关性随着叶位升高而降低，Y1的Chla+b含量与SPAD值的相关性最高，建立回归方程：y（Chla+b）=0.032x（SPAD）-0.141，决定系数（R ²）=0.699；Y3的叶绿素含量与SPAD值无显著相关性。最终选择该时期第一片新叶作为利用SPAD值无损预测甜瓜叶绿素含量的测量叶，为批量筛选甜瓜耐低温弱光材料提供可操作方案。

关键词: 甜瓜, 叶绿素, SPAD, 测量叶

Abstract: Two leaves and one new period was the sensitive period of melon to low temperature and low light condition, which period was always used to chlorophyll content determination and tolerance material selection. There were three leaves in this period, but no research mentioned about the definite measure leaf. To understand these and select the suitable measure leaf for chlorophyll content prediction by SPAD value determination, the chlorophyll content and SPAD value of three leaves of two leaves and one new period melon were collected and analyzed. The three leaves from bottom to up (Y1, Y2 and Y3) at different position of melon inbred line of P148 was selected to determinate and analyze the variation and correlation of SPAD value and chlorophyll content in two leaves and one new period by One-Way-ANOVA, Correlation and Regression program of SPSS 13.0 soft, respectively. The SPAD value and chlorophyll content were increased with increasing of leaves position. The Y3 showed the higher SPAD value and chlorophyll content than Y1 and Y2 significantly. The correlation between SPAD value and chlorophyll content was reduced with increasing of leaves position.The SPAD value and chlorophyll (a+b) content of Y1 had highest R value, the regression equation was established: y(Chla+b)=0.032x(SPAD)-0.141，R²=0.699. The first leaf was selected to determinate the SPAD value for chlorophyll content prediction to mass screen the low-temperature-and-light tolerance melon.

Key words: Melon, Chlorophyll, SPAD, Measure leaf

中图分类号:

S652.01

宋慧, 黄芸萍, 臧全宇, 张凌霄, 张香琴. 甜瓜幼苗不同叶位SPAD值与叶绿素含量的变化规律及相关性[J]. 华北农学报, 2019, 34(S1): 99-104. doi: 10.7668/hbnxb.20190255.

SONG Hui, HUANG Yunping, ZANG Quanyu, ZHANG Lingxiao, ZHANG Xiangqin. Variation and Correlation Analysis of SPAD Value and Chlorophyll Content of Leaves at Different Position in Melon Seedling[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 99-104. doi: 10.7668/hbnxb.20190255.

http://www.hbnxb.net/CN/Y2019/V34/IS1/99

参考文献

[1] 陈年来,王兴虎,安黎哲,王刚. 低温对厚皮甜瓜幼苗光合特性的影响[J]. 冰川冻土,2009,31(5):986-991.
Cen N L, Wang X H, An L Z, Wang G. Effect of low temperature on photosynthetic properties of melon seedlings[J]. Journal of Glaciology and Geocryology, 2009, 31(5):986-991.
[2] 乔宏宇,周艳丽,高红春. 低温弱光对不同砧木甜瓜嫁接苗生理指标的影响[J]. 东北农业科学,2016,41(2):84-87. doi:10.16423/j.cnki.1003-8701.2016.02.020.
Qao H Y, Zhou Y L, Gao H C. Effect of low temperature and poor light on some physiological indexes of melon seedlings grafted on different rootstocks[J]. Journal of Northeast Agricultural Sciences, 2016, 41(2):84-87. doi:10.16423/j.cnki.1003-8701.2016.02.020.
[3] 李琦. 低温弱光对甜瓜幼苗生理特性的影响[D]. 武汉:华中农业大学,2012.
L Q. Effects of low temperature and low light stress on physiological characteristics of melon seedlings[D]. Wuhan:Huazhong Agricultural University, 2012.
[4] 高青海,王亚坤,郭远远. 薄皮甜瓜种质资源苗期耐低温弱光鉴定及形态指标选择[J]. 浙江农业学报,2016,28(8):1360-1367. doi:10.3969/j.issn.1004-1524.2016.08.13.
Go Q H, Wang Y K, Guo Y Y. Identification for tolerance to low temperature and weak light and selection for morphologi-cal indexes of melon at seedling stage[J]. Acta Agriculturae Zhejiangensis, 2016, 28(8):1360-1367.
[5] 李静. 低温弱光胁迫对甜瓜幼苗生长及生理指标的影响[J]. 河南农业科学,2012,41(5):106-109. doi:10.15933/j.cnki.1004-3268.2012.05.029.
L J. Effects of chilling and low light on vegetative and physiological parameters of melon seedlings[J]. Journal of Henan Agricultural Sciences, 2012, 41(5):106-109.
[6] 吴秀娟,裴孝伯,吴良锁,王跃. 合肥地区春季大棚光温环境与番茄耐低温弱光指标的筛选[J]. 中国农学通报,2009,25(4):162-166.
W X J, Pei X B, Wu L S, Wang Y. Study on light and temperature in tunnel in the spring at Hefei and tolerant index for the low temperature and poor light of tomato[J]. Chinese Agricultural Science Bulletin, 2009, 25(4):162-166.
[7] 席晓飞,马正龙,许辉欣. 低温弱光对西葫芦幼苗的光合响应研究[J]. 甘肃农业科技,2015(4):30-33. doi:10.3969/j.issn.1001-1463.2015.04.009.
X X F, Ma Z L, Xu H X. Study on low temperature and weak light on photosynthetic response of Zucchini[J]. Gansu Agricultural Science and Technology, 2015(4):30-33.
[8] 闫世江,司龙亭,张建军,张继宁,刘洁. 黄瓜苗期低温弱光下耐寒性及光合参数遗传分析[J]. 烟台大学学报(自然科学版),2012,25(1):28-32. doi:10.13951/j.cnki.37-1213/n.2012.01.009.
Yn S J, Si L T,Zhang J J, Zhang J N, Liu J. Genetic analysis of chilling tolerance and photosynthetic parameters in low temperature and weak light on cucumber seedling[J]. Journal of Yantai University (Natural Science and Engineering Edition), 2012, 25(1):28-32.
[9] 张国斌,郁继华,许照耀,钟新榕. 低温弱光对辣椒幼苗叶绿素荧光参数的影响[J]. 甘肃农业大学学报,2004, 39(6):615-619. doi:10.13432/j.cnki.jgsau.2004.06.002.
Zang G B, Yu J H, Xu Z Y, Zhong X R. Effects of low temperature and poor light stress on the chlorophyll fluorescence parameters in the seedling of pepper[J]. Journal of Gansu Agricultural University, 2004, 39(6):615-619.
[10] 王薇,宋廷宇,王艳,董晓涛,柴晶,薛英. 番茄叶片SPAD值与叶绿素含量的相关性分析[J]. 北方园艺,2013(23):12-15.
Wng W, Song T Y, Wang Y, Dong X T, Chai J, Xue Y. Correlation analysis on chlorophyll content and SPAD value in tomato leaves[J]. Northern Horticulture, 2013(23):12-15.
[11] 宋廷宇,吴春燕,常雪,陈赫楠,王薇,郑丹,李爱梅. 西葫芦叶片SPAD值与叶绿素含量相关性分析[J]. 吉林农业科学, 2014,39(3):67-70.
Sng T Y, Wu C Y, Chang X, Chen H N, Wang W, Zheng D, Li A M. Analysis on correlation between leaf SPAD value and chlorophyll content of squash[J]. Journal of Jilin Agricultural Sciences, 2014, 39(3):67-70.
[12] 宋廷宇,程艳,何自涵,赵茹,吴春燕,张晓明,何启伟. 菜豆叶片SPAD值与叶绿素含量的相关性分析[J]. 山东农业科学, 2017,49(6):13-16. doi:10.14083/j.issn.1001-4942.2017.06.003.
Sng T Y, Cheng Y, He Z H, Zhao R, Wu C Y, Zhang X M, He Q W. Correlation analysis between SPAD value and chlorophyll content of bean leaf[J]. Shandong Agricultural Sciences, 2017, 49(6):13-16.
[13] 宋廷宇,何自涵,程艳,赵茹,吴春燕,张晓明. 菜心叶片SPAD值与叶绿素含量的相关性分析[J]. 东北农业科学, 2017,42(1):34-37. doi:10.16423/j.cnki.1003-8701.2017.01.010.
Sng T Y, He Z H, Cheng Y, Zhao R, Wu C Y, Zhang X M. Analysis on correlation between leaves SPAD value and chlorophyll content of flowering Chinese cabbage[J]. Journal of Northeast Agricultural Sciences, 2017, 42(1):34-37.
[14] 程艳,吴春燕,张晓旭,王娜,孙凯,于娅,王飞,张晓明,宫国辉. 蕹菜叶片SPAD值与叶绿素含量的相关性分析[J]. 东北农业科学,2018,43(4):44-47. doi:10.16423/j.cnki.1003-8701.2018.04.011.
Ceng Y, Wu C Y, Zhang X X, Wang N, Sun K, Yu Y, Wang F, Zhang X M, Gong G H. Analysis of correlation between chlorophyll content and SPAD value in water spinach leaves[J]. Journal of Northeast Agricultural Sciences, 2018, 43(4):44-47.
[15] 乔润雨,刘文锋,刘泽群,唐文娇,万宁,孙秋寒,于瑞琦,邱念伟. 绿色蔬菜叶片叶绿素含量与SPAD值相关性研究[J]. 国土与自然资源研究,2018(1):80-82. doi:10.16202/j.cnki.tnrs.2018.01.024.
Qao R Y, Liu W F, Liu Z Q, Tang W J, Wan N, Sun Q H, Yu R Q, Qiu N W. Correlation analysis of chlorophyll content and SPAD value in green vegetable leaves[J]. Territory and Natural Resources Study, 2018(1):80-82.
[16] 宋廷宇,陈赫楠,常雪,吴春燕,王薇,郑丹,叶新鹏. 2个薄皮甜瓜叶片SPAD值与叶绿素含量的相关性分析[J]. 江苏农业科学,2014,42(4):127-129.doi:10.3969/j.issn.1002-1302.201404.048.
Sng T Y, Chen H N, Chang X, Wu C Y, Wang W, Zheng D, Ye X P. Correlation analysis of chlorophyll content and SPAD value in melon leaves[J]. Journal of Jiangsu Agricultural Sciences, 2014, 42(4):127-129.
[17] 胡国智,熊韬,张炎,冯炯鑫,吴海波,李青军. 甜瓜主茎叶不同叶位SPAD值空间分布与氮素营养诊断[J]. 中国土壤与肥料,2017(1):80-85. doi:10. 11838/sfsc. 20170114.
H G Z, Xiong T, Zhang Y, Feng J X, Wu H B, Li Q J. Spatial distribution and nitrogen diagnosis of SPAD value for different leaves position on main stem of muskmelon[J]. Soils and Fertilizers Sciences in China, 2017(1):80-85.
[18] 胡静,毛罕平,左志宇,李青林. 黄瓜叶片上SPAD值的空间分布及氮素诊断的位点选择[J].江苏大学学报(自然科学版)[J]. 2015,36(4):411-417. doi:10.3969/j.issn.1671-7775.2015.04.007.
H J, Mao H P, Zuo Z Y, Li Q L. Spatial distribution of SPAD value and determination of suitable leaf for N diagnosis in cucumber[J]. Journal of Jiangsu University (Natural science edition),2015, 36(4):411-417.
[19] 肖关丽,郭华春. 不同温光条件下马铃薯不同叶位叶SPAD值变化规律研究[J]. 中国马铃薯,2007,21(3):146-148.doi:10.3969/j.issn.1672-3635.2007.03.005.
Xao G L, Guo Huachun. SPAD value of leaf at different positions on main stem in potato as influenced by temperature and photoperiod[J].Chinese Potato Journal,2007,21(3):146-148.
[20] 唐懿,余雪娜,何娟,张恩泽,兰静宇,李焕秀. 外源褪黑素对低温弱光胁迫下茄子幼苗生理特性的影响[J]. 湖南农业大学学报(自然科学版),2017,43(3):257-261. doi:10.13331/j.cnki.jhau.2017.03.006.
Tng Y, Yu X N, He J, Zhang E Z, Lan J Y, Li H X. Effects of exogenous melatonin on physiological properties of eggplant (Solanum melongena L.) seedlings under the stress of low light and low temperature[J]. Journal of Hunan Agricultural University (Natural Sciences), 2017, 43(3):257-261.
[21] 聂书明,杜中平,王丽慧,徐海勤. 低温弱光对辣椒叶片光合特性和生理特性的影响[J]. 西南农业学报,2016,29(10):2319-2323. doi:10.16213/j.cnki.scjas.2016.10.012.
Ne S M, Du Z P, Wang L H, Xu H Q. Effects of photoaynthesis characteristics of pepper seedlings under low temperature and weak light stress[J]. Southwest China Journal of Agricultural Sciences, 2016, 29(10):2319-2323.

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