镉对薄皮甜瓜幼苗生长和生理生化特性的影响

doi:10.7668/hbnxb.20190467

摘要/Abstract

摘要： 为了研究不同浓度镉胁迫对不同薄皮甜瓜品种幼苗生长和生理特性的影响，在前期试验的基础上，采用水培方法，以薄皮甜瓜品种X-T-G （相对高积累品种）和IVF-28（相对低积累品种）为材料，通过设置0，30，60，100 mg/L 4种不同镉浓度，研究不同镉胁迫浓度下X-T-G和IVF-28薄皮甜瓜品种地上部、根系生长和生理生化指标的变化。结果表明：X-T-G和IVF-28的株高、茎粗、总根长、根表面积、根体积、根尖数、分形维数均随镉胁迫处理的浓度增加而减小，其中X-T-G降幅与IVF-28相比较低。随着镉胁迫处理浓度的增加，2个供试的薄皮甜瓜品种的生理也发生了改变，其中SOD、MDA和H₂O₂含量均随镉胁迫处理浓度的升高而增大，POD活性和GSH含量在60 mg/L CdCl₂处理时达到最大，而CAT活性和PRO含量在30 mg/L CdCl₂处理时达到最大，其中X-T-G的生理生化指标高于IVF-28。综上可知，薄皮甜瓜品种X-T-G幼苗在镉胁迫下，幼苗对镉胁迫的耐受性高于IVF-28。

关键词: 薄皮甜瓜, 镉, 幼苗, 生长, 生理

Abstract: In order to study the effects of different concentrations of cadmium stress on the growth and physiological characteristics of different thin-skinned melon varieties at seedling stage,hydroponic methods were conducted with based on previous experiments,and the thin-skinned melon varieties XTG(relatively high accumulation species)and IVF-28(relatively low accumulation species)were selected as trial cultivars. Four different CdCl₂ concentrations of 0,30,60,and 100 mg/L were set,the changes of shoot growth,root growth and physiological and biochemical indexes under different Cd stress concentrations were studied. The results showed that the plant height,stem thickness,total root length,root surface area,root volume,root tip number,and fractal dimension of XTG and IVF-28 all decreased as the concentration of Cd stress increased. And the decrease of XTG was lower than that of IVF-28. With the increase of Cd stress treatment concentration,the physiology of the two tested thin-skinned melon varieties also changed. The SOD,MDA and H₂O₂ contents increased with the increase of Cd stress treatment concentration,and the POD activity and GSH content reached the maximum under 60 mg/L CdCl₂ treatment,while CAT activity and PRO content reached the maximum when 30 mg/L CdCl₂ treatment,in which the physiological and biochemical indicators of XTG were higher than IVF-28. The results showed that X-T-G,a thin-skinned melon variety,had a lower decline in growth-related indicators than IVF-28 under Cd stress,and physiological indicators were higher than IVF-28,indicating that X-T-G had high tolerance to Cd stress than IVF-28.

Key words: Oriental melon, Cadmium, Seedling, Growth, Physiological

中图分类号:

S652.01

王志伟, 李涵, 梁少华, 楚箫, 孙小武. 镉对薄皮甜瓜幼苗生长和生理生化特性的影响[J]. 华北农学报, 2020, 35(1): 81-88. doi: 10.7668/hbnxb.20190467.

WANG Zhiwei, LI Han, LIANG Shaohua, CHU Xiao, SUN Xiaowu. Effects of Cadmium on Growth and Some Physiological of Oriental Melon Seedlings[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 81-88. doi: 10.7668/hbnxb.20190467.

http://www.hbnxb.net/CN/Y2020/V35/I1/81

参考文献

[1] 鲜靖苹,柴澍杰,王勇,牛奎举,董文科,马晖玲,张然.镉胁迫对草地早熟禾生长与生理代谢的影响[J].核农学报, 2019, 33(1):176-186.doi:10.11869/j.issn.100-8551.2019.01.0176.
Xian J P, Chai S J, Wang Y, Niu K J, Dong W K, Ma H L, Zhang R. Effect of cadmium stress on growth and physiological metabolism of kentucky bluegrass[J].Journal of Nuclear Agricultural Sciences,2019,33(1):176-186.
[2] 冯建鹏,史庆华,王秀峰,洪艳艳.镉对黄瓜幼苗光合作用、抗氧化酶和氮代谢的影响[J].植物营养与肥料学报, 2009, 15(4):970-974.doi:10.3321/j.issn:1008-505X.2009.04.036.
Feng J P, Shi Q H, Wang X F, Hong Y Y. Effects of cadmium on photosynthesis,antioxidant enzyme and nitrogen metabolism of cucumber seedlings[J].Journal of Plant Nutrition and Fertilizers, 2009, 15(4):970-974.
[3] 王志伟,邹甜,孙波,龚思,陈泽南,孙小武.镉在薄皮甜瓜植株中的分布及低积累品种筛选[J].中国蔬菜, 2018(11):55-59.
Wang Z W, Zou T, Sun B, Gong S, Chen Z N, Sun X W. Cadmium distribution and screening of low cadmium accumulation oriental melon cultivars[J].China Vegetables, 2018(11):55-59.
[4] 吴志超. 高低镉积累油菜品种筛选及其生化机制研究[D].武汉:华中农业大学, 2015.doi:10.7666/d.Y2803169.
Wu Z C. Screening of high/low cadmium accumulation Brassica napus cultivars and research on the biochemical mechanisms[D].Wuhan:Huazhong Agricultural University,2015.
[5] 刘灿玉,夏杰,韩敏,曹逼力,徐坤.镉对姜叶片活性氧代谢影响的时间和剂量效应[J].园艺学报, 2016, 43(5):927-934.doi:10.16420/j.issn.0513-353x.2016-0138.
Liu C Y, Xia J, Han M, Cao B L, Xu K. Time and does effects of cadmium on active oxygen metabolism in ginger leaves[J].Acta Horticulturae Sinica, 2016, 43(5):927-934
[6] 沈雪婷,邢雁,卢迪,吴明,包军,于建军.镉污染对烟草的毒害及调控措施研究进展[J]. 江西农业学报, 2014, 26(9):57-60.doi:10.19386/j.cnki.jxnyxb.2014.09.014.
Shen X T, Xing Y, Lu D, Wu M, Bao J, Yu J J. Research progress in poisoning of cadmium pollution to tobacco and its control measures[J].Acta Agriculturae Jiangxi, 2014, 26(9):57-60.
[7] 朱志勇,郝玉芬,吴金芝,黄明,刘英杰,李友军.镉胁迫对不同小麦品种幼苗生长以及Cd²⁺、Zn²⁺、Mn²⁺吸收和积累的影响[J].水土保持学报, 2011, 25(2):209-213.
Zhu Z Y, Hao Y F, Wu J Z, Huang M, Liu Y J, Li Y J. Effects of Cd²⁺ stress on the seedling growth,Cd²⁺,Zn²⁺ and Mn²⁺ uptake of different wheat cultivars[J].Journal of Soil and Water Conservation, 2011, 25(2):209-213.
[8] 张永平,沈若刚,姚雪琴,陈幼源. 镉胁迫对甜瓜幼苗抗氧化酶活性和光合作用的影响[J]. 中国农学通报, 2015, 31(34):82-88.
Zhang Y P, Shen R G, Yao X Q, Chen Y Y. Effects of cadmium stress on antioxidant enzyme activities and photosynthesis in melon seedlings[J].Chinese Agricultural Science Bulletin,2015, 31(34):82-88.
[9] 周建,张毅川,齐安国,王彩云.镉胁迫对刺槐幼苗的生长、镉离子富集及其细胞分布的影响[J]. 河南科技学院学报(自然科学版),2018, 46(1):1-7.doi:10.3969/j.issn.1008-7516.2018.01.001.
Zhou J, Zhang Y C, Qi A G, Wang C Y. Effects of cadmium stress on growth,ions accumulation and cellular distribution in Robinia pseudoacacia seedlings[J].Journal of Henan Institute of Science and Technology(Natural Science Edition), 2018, 46(1):1-7.
[10] 何梨香,黄运湘,黄楚瑜,刘利杉,龙祥,罗琳. 圆叶决明对镉胁迫的生理响应[J]. 草业学报, 2016, 25(2):198-204.doi:10.11686/cyxb2015158.
He L X, Huang Y X, Huang C Y, Liu L S, Long X, Luo L. Physiological reponse of Chamaecrista rotundifolia to cadmium exposure[J]Acta Prataculturae Sinica,2016,25(2):198-204.
[11] 闫道良,余婷,徐菊芳,黄坚钦.盐胁迫对海滨锦葵生长及Na⁺、K⁺离子积累的影响[J]. 生态环境学报, 2013, 22(1):105-109.doi:10.16258/j.cnki.1674-5906.2013.01.004.
Yan D L, Yu T, Xu J F,Huang J Q.Effect of salt stress on the growth and accumulation of Na⁺,K⁺ in seedlings of Kosteletzkya virginica[J].Ecology and Environmental Sciences, 2013, 22(1):105-109.
[12] 唐晓倩,刘广全. NaCl胁迫对圆柏幼苗生长和离子吸收及分配的影响[J]. 西北植物学报, 2017, 37(7):1372-1380.doi:10.7606/j.issn.1000-4025.2017.07.1372.
Tang X Q, Liu G Q. Effects of NaCl stress on the growth ion absorption and distribution of Juniperus chinensis seedlings[J].Acta Botanica-Occidentalia Sinica,2017,37(7):1372-1380.
[13] 刘正祥,张华新,杨秀艳,刘涛,狄文彬.NaCl胁迫下沙枣幼苗生长和阳离子吸收、运输与分配特性[J]. 生态学报, 2014, 34(2):326-336.doi:10.5846/stxb201303270530.
Liu Z X, Zhang H X, Yang X Y, Liu T, Di W B. Growth,and cationic absorption,transportation and allocation of Elaeagnus angustifolia seedlings under NaCl stress[J].Atca Ecologica Sinica, 2014, 34(2):326-336.
[14] 王树凤,胡韵雪,孙海菁,施翔,潘红伟,陈益泰.盐胁迫对2种栎树苗期生长和根系生长发育的影响[J].生态学报, 2014, 34(4):1021-1029.doi:10.5846/stxb201209291363.
Wang S F, Hu Y X, Sun H Q, Shi X, Pan H W, Chen Y T. Effects of salt stress on growth and root development of two oak seedlings[J].Atca Ecologica Sinica, 2014, 34(4):1021-1029.
[15] 叶亚新,金琎,陈佳佳,徐莉.镉胁迫下萝卜幼苗根、茎、叶保护酶活性的比较[J]. 江苏农业科学, 2008(3):131-135.doi:10.3969/j.issn.1002-1302.2008.03.047.
Ye Y X, Jin J, Chen J J, Xu L. Comparison of the protective enzymes activities in root, stem and leaf of radish seedlings under cadmium stress[J].Jiangsu Agricultural Sciences, 2008(3):131-135.
[16] 铁柏清,袁敏,唐美珍,秦普丰,杨永兴.重金属单一污染对龙须草生长与生理生化特性的影响[J].中国生态农业学报, 2007, 15(2):99-103.
Tie B Q, Yuan M, Tang M Z, Qin P F, Yang Y X. Effects of single heavy metal pollution on the growth and physiological and biochemical characteristics of Eulaliosis binata[J].Chinese Journal of Eco-Agriculture, 2007, 15(2):99-103.
[17] 惠俊爱,党志.土壤不同镉浓度对玉米CT38生长及抗氧化酶活性的影响[J].生态环境学报, 2014, 23(5):884-889.doi:10.16258/j.cnki.1674-5906.2014.05.027.
Hui J A,Dang Z.Effects of different cadmium concentrations in soil on growth and antioxidant enzyme activities of maize CT38[J].Ecology and Environmental Sciences,2014,23(5):884-889.
[18] 谢惠玲,陈爱萍,张凤英,熊颖,陈艳玲,刘杰,周丽英,林瑞余. 紫苏对不同浓度镉胁迫的生理响应[J].中国生态农业学报, 2011, 19(3):672-675.doi:10.3724/SP.J.1011.2011.00672.
Xie H L, Chen A P, Zhang F Y, Xiong Y, Chen Y L, Liu J, Zhou L Y, Lin R Y. Physiological response of Perilla frutescens (L.)Britt.to cadimium[J]. Chinese Journal of Eco-Agriculture, 2011, 19(3):672-675.
[19] Mobin M, Khan N A. Photosynthetic activity, pigment composition and antioxidative response of two mustard (Brassica juncea) cultivars differing in photosynthetic capacity subjected to cadmium stress[J]. Journal of Plant Physiology, 2007, 164(5):601-610.doi:10.1016/j.jplph.2006.03.003.
[20] 郑世英,张秀玲,王丽燕,商学芳.Pb²⁺,Cd²⁺胁迫对棉花保护酶及丙二醛含量的影响[J].河南农业科学, 2007(8):43-45,63.doi:10.15933/j.cnki.1004-3268.2007.08.016.
Zheng S Y,Zhang X L,Wang L Y,Shang X F. Effects of Pb²⁺ and Cd²⁺ stress on the protective enzymes activity and MDA contents of cotton[J]. Journal of Henan Agricultural Sciences,2007(8):43-45,63.
[21] 曹红梅,闫晓丽. 香根草和风车草对重金属镉胁迫的生理生化反应研究[J].现代农业科技, 2015(11):226-227.doi:10.3969/j.issn.1007-5739.2015.11.142.
Cao H M, Yan X L. Response of physiology and biochemistry of Vetiver zizanioides and Cyperus altemifoliu to heavy metal cadmium stress[J]. Modern Agricultural Science and Technology,2015(11):226-227.
[22] 温瑀,穆立蔷.土壤铅、镉胁迫对4种绿化植物生长、生理及积累特性的影响[J].水土保持学报, 2013, 27(5):234-238.doi:10.13870/j.cnki.stbcxb.2013.05.003.
Wen Y, Mu L Q. Effects of soil Pb, Cd stress on the growth, physiologcal and accumulating characteristics of four ornamental trees[J].Journal of Soil and Water Conservation, 2013, 27(5):234-238.
[23] 张凤琴,王友绍,董俊德,孙翠慈,殷建平. 重金属污水对木榄幼苗几种保护酶及膜脂质过氧化作用的影响[J].热带海洋学报, 2006, 25(2):66-70.doi:10.3969/j.issn.1009-5470.2006.02.012.
Zhang F Q, Wang Y S, Dong J D, Sun C C, Yin J P. Effects of wastewater borne heavy metals on some protective enzymes and lipid peroxidation of Bruguiera gymnorrhiza seedling[J].Journal of Tropical Oceanography,2006, 25(2):66-70.
[24] 张廷婷,闫彩霞,李春娟,单世华,万书波,杨志艺.花生对镉胁迫的生理响应[J].山东农业科学, 2013, 45(12):48-51, 56.doi:10.14083/j.issn.1001-4942.2013.12.025.
Zhang Y T, Yan C X, Li C J, Shan S H, Wan S B, Yang Z Y. Physiological response of peanut to cadmium stress[J].Shandong Agricultural Sciences, 2013, 45(12):48-51, 56.
[25] 高夕彤,李硕,贾娟,李岩,杨志新. 不同番茄品种对镉胁迫的耐抗性差异研究[J]. 华北农学报, 2018, 33(2):169-176.doi:10.7668/hbnxb.2018.02.024.
Gao X T, Li S, Jia J, Li Y, Yang Z X. Study on resistance of different tomato varieties to cadmium stress[J].Acta Agriculturae Boreali-Sinica, 2018, 33(2):169-176.
[26] 王欣欣,邓明华,龚明,刘颖,陈凯,文锦芬. Cd²⁺胁迫对小桐子幼苗叶片抗氧化系统的影响[J]. 西北植物学报, 2016, 36(3):527-533.doi:10.7606/j.issn.1000-4025.2016.03.0527.
Wang X X, Deng M H, Gong M, Liu Y, Chen K, Wen J F. Effect of Cd²⁺ stress on antioxidant system in the leaves of Jatropha curcas seedlings[J]. Acta Botanica Boreali-Occidentalia Sinica, 2016, 36(3):527-533.
[27] 孙亚莉,刘红梅,徐庆国. 镉胁迫对不同水稻品种苗期光合特性与生理生化特性的影响[J]. 华北农学报, 2017, 32(4):176-181.doi:10.7668/hbnxb.2017.04.028.
Sun Y L, Liu H M, Xu Q G. Effect of cadmium stress on photosynthetic characteristics and physiological and biochemical traits during seedling stage of different rice cultivars[J]. Acta Agriculturae Boreali-Sinica, 2017, 32(4):176-181.
[28] 唐雪东,刘晓嘉,窦森,李亚东. 3种果树幼苗对镉胁迫的响应[J].东北林业大学学报, 2009, 37(9):27-28, 32.doi:10.13759/j.cnki.dlxb.2009.09.001.
Tang X D, Liu X J, Dou S, Li Y D. Responses of three species of fruit tree seedlings to cadmium stress[J].Journal of Northeast Forestry University,2009, 37(9):27-28, 32.
[29] 吴桂容. 重金属Cd对桐花树幼苗生理生态效应及土壤酶的影响研究[D].厦门:厦门大学, 2007.doi:10.7666/d.y1344841.
Wu G R. Effects of heavy metal Cd on physiological ecological effect and soil enzyme of tonghua tree seedlings[D].Xiamen:Xiamen University,2007.
[30] 欧阳燕莎,刘爱玉,李瑞莲,刘浩然.高低镉积累棉花品种生理生化特性研究[J]. 华北农学报, 2017, 32(6):170-174.doi:10.7668/hbnxb.2017.06.025.
Ouyang Y S, Liu A Y, Li R L, Liu H R. Study on physiological and biochemical characteristics of cotton varieties accumulated by high and low cadmium[J]. Acta Agriculturae Boreali-Sinica, 2017, 32(6):170-174.
[31] Fu X P,Dou C M,Chen Y X,Chen X C,Shi J Y,Yu M G,Xu J. Subcellular distribution and chemical forms of cadmium in Phytolacca americana L.[J]. Journal of Hazardous Materials, 2011, 186(1):103-107.doi:10.1016/j.jhazmat.2010.10.122.

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