华北农学报 ›› 2025, Vol. 40 ›› Issue (3): 97-103. doi: 10.7668/hbnxb.20195431

所属专题: 土壤肥料 蔬菜专题 栽培生理

• 耕作栽培·生理生化 • 上一篇    下一篇

氨基酸水溶肥对芹菜苗期叶绿素含量及代谢有关基因表达的影响

苑雪云1, 陈晨1, 鄢悦1, 危咏菊1, 王俐翔1,2, 孙旭1, 孙悦华1, 李亚鹏1, 刘佩卓1, 熊爱生1,2,   

  1. 1 南京农业大学 园艺学院,作物遗传与种质创新利用全国重点实验室,农业农村部华东地区园艺作物生物学与种质创制重点实验室,江苏 南京 210031
    2 宿迁市设施园艺研究院(南京农业大学 宿迁研究院),江苏 宿迁 223800
  • 收稿日期:2024-10-26 出版日期:2025-06-28
  • 通讯作者:
    熊爱生(1975—),男,江苏溧水人,教授,博士,博士生导师,主要从事蔬菜遗传育种和全产业示范推广工作。
  • 作者简介:

    苑雪云(2003—),女,河南周口人,硕士,主要从事蔬菜遗传育种与分子生物学研究。

  • 基金资助:
    中央农业重大技术协同推广计划项目(2022-ZYXT-01-3); 大学生创新创业计划项目(202314XX030); 江苏省重点研发计划项目(BE2022386); 江苏省种业振兴揭榜挂帅项目[JBGS(2021); 宿迁市重点研发计划项目(L202302); 江苏高校优势学科建设项目(PAPD)

Effects of Amino Acid Type Water-soluble Fertilizer on Chlorophyll Content and Expression of Genes Related to Metabolism in Celery at Seedling Stage

YUAN Xueyun1, CHEN Chen1, YAN Yue1, WEI Yongju1, WANG Lixiang1,2, SUN Xu1, SUN Yuehua1, LI Yapeng1, LIU Peizhuo1, XIONG Aisheng1,2,   

  1. 1 College of Horticulture,Nanjing Agricultural University,State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization,Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,Nanjing 210031,China
    2 Suqian Facility Horticulture Research Institute(Suqian Research Institute of Nanjing Agricultural University),Suqian 223800,China
  • Received:2024-10-26 Published:2025-06-28

摘要:

探索氨基酸水溶肥对芹菜叶绿素含量、比例的影响,及其对叶绿素代谢相关基因表达的调控作用,以期为高品质富色素芹菜生产中氨基酸水溶肥叶面肥的合理施用提供理论依据。以宁芹1号为材料,设置不同浓度氨基酸水溶肥叶面喷施处理,测定叶片及叶柄叶绿素a、叶绿素b及总叶绿素含量并分析,通过荧光定量PCR(RT-qPCR)测定叶绿素代谢有关基因相对表达水平。氨基酸水溶肥对本芹叶绿素积累及相关基因相对表达水平的影响与处理浓度及叶片部位有关,500 μL/L氨基酸水溶肥处理可促进叶片和叶柄中叶绿素a、叶绿素b及总叶绿素含量增加,降低叶柄中叶绿素a/b,诱导叶绿素合成相关基因(AgHEMAAgHEMBAgCHLMAgPORAgCAO)表达并显著下调各降解相关基因(AgPAOAgPPH)表达水平;1 000 μL/L氨基酸水溶肥处理在一定程度上阻碍叶绿素积累,提高叶片中叶绿素a/b,显著下调各叶绿素合成相关基因及叶绿素降解相关基因AgPPH的表达水平。适宜浓度的氨基酸水溶肥叶面肥可调控叶绿素代谢相关基因表达水平,提高芹菜苗期叶绿素含量。

关键词: 芹菜, 氨基酸水溶肥, 叶面喷施, 叶绿素代谢, 苗期

Abstract:

Explore the effects of amino acid type water-soluble fertilizer on chlorophyll content and proportion of celery,and the regulation of gene expression related to chlorophyll metabolism,which provides a theoretical basis for the rational application of amino acid type water-soluble fertilizer foliar fertilizer in the production of high-quality pigment-rich celery.The celery variety Ningqin 1 was sprayed with different concentrations of amino acid type water-soluble fertilizer,the contents of chlorophyll a,chlorophyll b and total chlorophyll in leaf blades and petioles were determined and analyzed,and the relative expression levels of genes related to chlorophyll metabolism were determined by fluorescence quantitative PCR(RT-qPCR).The effects of amino acid type water-soluble fertilizer on chlorophyll accumulation and relative expression levels of related genes were related to the treatment concentration and leaf location.Treatment with 500 μL/L amino acid type water-soluble fertilizer increased the contents of chlorophyll a,chlorophyll b and total chlorophyll in leaf blades and petioles,and decreased the ratio of chlorophyll a/b in petioles.The expression of chlorophyll synthesis-related genes(AgHEMA,AgHEMB,AgCHLM,AgPOR and AgCAO)was induced,and the expression levels of degradation related genes(AgPAO and AgPPH)were significantly down-regulated.Treatment with 1 000 μL/L amino acid type water-soluble fertilizer inhibited chlorophyll accumulation to a certain extent,increased chlorophyll a/b ratio in leaf blades,and significantly down-regulated the expression levels of genes related to chlorophyll synthesis and chlorophyll degradation AgPPH.The suitable concentration of amino acid type water-soluble fertilizer foliar fertilizer can improve the chlorophyll content of celery at seeding stage by regulating the expression level of genes related to chlorophyll metabolism.

Key words: Celery, Amino acid type water-soluble fertilizer, Foliar spray, Chlorophyll metabolism, Seedling stage

中图分类号: 

引用本文

苑雪云, 陈晨, 鄢悦, 危咏菊, 王俐翔, 孙旭, 孙悦华, 李亚鹏, 刘佩卓, 熊爱生. 氨基酸水溶肥对芹菜苗期叶绿素含量及代谢有关基因表达的影响[J]. 华北农学报, 2025, 40(3): 97-103. doi: 10.7668/hbnxb.20195431.

YUAN Xueyun, CHEN Chen, YAN Yue, WEI Yongju, WANG Lixiang, SUN Xu, SUN Yuehua, LI Yapeng, LIU Peizhuo, XIONG Aisheng. Effects of Amino Acid Type Water-soluble Fertilizer on Chlorophyll Content and Expression of Genes Related to Metabolism in Celery at Seedling Stage[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 97-103. doi: 10.7668/hbnxb.20195431.