小麦种质CH7015中抗白粉病基因的SSR定位

doi:10.7668/hbnxb.20190023

华北农学报 ›› 2019, Vol. 34 ›› Issue (6): 203-208. doi: 10.7668/hbnxb.20190023

所属专题： 小麦；生物技术；

• 资源环境·植物保护 • 上一篇下一篇

小麦种质CH7015中抗白粉病基因的SSR定位

郭慧娟¹, 贾举庆², 李欣¹, 乔麟轶¹, 阎晓涛¹, 任永康¹, 常利芳¹, 张树伟¹, 畅志坚¹, 张晓军¹

1. 山西省农业科学院作物科学研究所, 作物遗传与分子改良山西省重点实验室, 农业部黄土高原作物基因资源与种质创制重点实验室, 山西省主要农作物种质创新与分子育种重点科技创新平台, 山西太原 030031;
2. 山西农业大学农学院, 山西太谷 030801

收稿日期:2019-07-21 出版日期:2019-12-28
通讯作者: 张晓军(1977-),男,山西临汾人,副研究员,硕士,硕士生导师,主要从事植物染色体工程及分子育种研究。
作者简介:郭慧娟(1983-),女,山西汾阳人,助理研究员,硕士,主要从事作物种质资源创新与遗传育种研究。
基金资助:
国家重点研发计划项目（2017YFD0100600）；山西省农业科学院项目（YGG17123；YCX2018D2YS01）；山西省重点研发计划项目（201803D221018-5；201703D211007；201803D421020）；山西省重点科技创新平台项目（201605D151002）

Mapping of Resistance Gene to Powdery Mildew in Wheat Cultivar CH7015 Using SSR Markers

GUO Huijuan¹, JIA Juqing², LI Xin¹, QIAO Linyi¹, YAN Xiaotao¹, REN Yongkang¹, CHANG Lifang¹, ZHANG Shuwei¹, CHANG Zhijian¹, ZHANG Xiaojun¹

1. Institute of Crop Sciences, Shanxi Academy of Agricultural Sciences, Shanxi Key Laboratory of Crop Genetics and Molecular Improvement, Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau of Ministry of Agriculture, Shanxi Key Scientific and Technological Innovation Platform for Germplasm Creation and Molecular Breeding of Main Crops, Taiyuan 030031, China;
2. College of Agriculture, Shanxi Agricultural University, Taigu 030801, China

Received:2019-07-21 Published:2019-12-28

摘要/Abstract

摘要： 为了明确小麦与八倍体小偃麦远缘杂交培育的小麦新种质CH7015中抗白粉病基因的来源及其在染色体上的具体位置。将CH7015与感病品种台长29杂交，对其F₁、BC₁、F₂群体接种白粉病，进行抗病性鉴定和抗感杂交后代的遗传分析，选取分布于小麦21对染色体上的825对SSR引物，采用群体分离分析法（BSA）对台长29×CH7015的F₂群体进行标记筛选。结果显示，CH7015抗性受1对显性核基因控制，其抗白粉病基因PmCH7015可能来源于中间偃麦草。通过抗感基因池和群体筛选，获得5个连锁标记，分别为：Xwmc657、Xgpw2328、Xwmc68、Xgpw4079和Xgpw7272。其中，Xwmc68和Xgpw4079位于PmCH7015两侧，遗传距离分别为8.2，1.4 cM。中国春缺体-四体和双端体的验证结果将抗病基因定位于小麦4B染色体的短臂上（4BS）。综上所述，由于小麦4BS染色体上尚无有关抗白粉病基因的报道，因此，推测PmCH7015是一个新发现的抗白粉病基因位点，其抗性可能来源于中间偃麦草。

关键词: 小麦, 抗白粉病基因, 中间偃麦草, 分子作图, SSR标记

Abstract: In order to disclose the genetic mechanisms behind this resistance and identify the powdery mildew resistance gene in CH7015, a new wheat breeding line derived from five hybrids of common wheat crossed with wheat-Thi.intermedium partial amphidiploids,the CH7015 was crossed with Taichang 29 and developed segregating populations F₁, BC₁, F₂ generations. Using 825 pairs SSR primers distributed on 21 pairs of chromosome of common wheat to analysis the F₂ populations of Taichang 29×CH7015. The results of genetic analysis showed that the resistance gene to powdery mildew in CH7015 is a single dominant gene tentatively named pmCH7015, which may be derived from Thi.intermedium. Five SSR markers, i.e., Xwmc657,Xgpw2328,Xwmc68,Xgpw4079 and Xgpw7272, were screened for linking with PmCH7015. And the genetic distances of PmCH7015 with the flanking markers were 8.2 cM to Xwmc68 and 1.4 cM to Xgpw4079. Chinese Spring ditelosomic and nulli-tetrasomic lines were used to locate the gene of PmCH7015 on wheat chromosome 4BS. Since no related report for Bgt resistance gene in 4BS of wheat, so far, the results of the study indicated that PmCH7015 is a novel Bgt resistance gene which probably derived from Thi.intermedium.

Key words: Wheat, Powdery mildew resistance gene, Thinopyrum intermedium, Molecular mapping, SSR marker

中图分类号:

S435
Q78

郭慧娟, 贾举庆, 李欣, 乔麟轶, 阎晓涛, 任永康, 常利芳, 张树伟, 畅志坚, 张晓军. 小麦种质CH7015中抗白粉病基因的SSR定位[J]. 华北农学报, 2019, 34(6): 203-208. doi: 10.7668/hbnxb.20190023.

GUO Huijuan, JIA Juqing, LI Xin, QIAO Linyi, YAN Xiaotao, REN Yongkang, CHANG Lifang, ZHANG Shuwei, CHANG Zhijian, ZHANG Xiaojun. Mapping of Resistance Gene to Powdery Mildew in Wheat Cultivar CH7015 Using SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 203-208. doi: 10.7668/hbnxb.20190023.

http://www.hbnxb.net/CN/Y2019/V34/I6/203

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