摘要： 根据已经获得的中原牡丹品种资源的初级核心种质120个品种的表型性状信息和ISSR、AFLP分子标记遗传信息,采用单一表型性状、分子、表型结合分子信息聚类压缩取样及随机取样的方法,进行了构建中原牡丹品种核心种质的研究.结果表明:采用表型信息结合两种分子标记信息聚类压缩的方法构建核心种质的方法最好.经检验,各种检验指数及品种间平均遗传距离均高于初级核心种质,也均高于保留种质,表型保留比例达99%,多态性位点及百分率、平均观测等位基因数的保留率达到了94.5%以上.核心种质很好地代表了中原牡丹品种初级核心种质的遗传多样性,可为杂交育种和种质保存提供参考.

关键词: 牡丹, 核心种质, 表型性状, 分子标记

Abstract: Based on the data of morphological and agronomic traits and the molecular marker information of ISSR and AFLP of the primary core collection including 120 tree peony cultivars from Central China,the study of how to establish a core collection of tree peonies from Central China was carried out.The result showed that the best method for establishing the core collection was from the UPGMA cluster drawing samples which combined the phenotypic traits with two kinds of molecular marker information.Test results showed that some testing parameters and the average genetic distance between cultivars of the core collection were bigger than the primary collection and the reservation collection.The ratio of retained phenotype traits exceeded 99%.The retention rates of polymorphic locus and the average allele number observed were more than 94.95%.Therefore,the core collection could furthest represent the genetic diversity of the primary collection and made a basement to hybrid breeding and germplasm reservation of Chinese tree peony cultivars.

Key words: Chinese tree peony, Core collection, Phenotypic traits, Molecular mark

中图分类号: 

• S02.4