摘要： 为进一步探索中原牡丹品种核心种质的遗传多样性,采用SRAP 分子标记技术对58个中原牡丹核心种质进行了遗传多样性分析。结果表明,23对引物组合共扩增出稳定清晰的条带595条,其中多态性条带377条,占64.3%;每对引物组合的平均条带数和多态性带数分别为25.9,16.4条;供试品种间成对遗传相似系数为0.567~0.894,平均为0.752;采用UPGMA法,在遗传相似系数为0.692处,供试材料聚为7个类群,表明中原牡丹核心种质具有较丰富的遗传多样性。聚类结果显示,单花类和台阁类有分别聚在一起的趋势,但与牡丹花型分类并不完全一致;花色相近的品种没有聚到一类中,而是分散在各个类别中,即聚类结果与牡丹品种的花型、花色等性状没有明显的关系,说明重瓣性低的品种与重瓣性高的品种间存在较大的遗传差异。这可能与牡丹长期引种、选择和反复杂交等导致的品种来源复杂有关。

关键词: 牡丹, 核心种质, SRAP, 遗传多样性

Abstract: In order to explore the genetic diversity of core collection of tree peony cultivars from central China, the technology of SRAP molecular marker was used in the study.Based on the technology of SRAP molecular marker, the genetic diversity of the core collection of tree peony cultivars from central China.The results showed that 23 SRAP primer combinations were employed to evaluate the genetic diversity of the core collection including 58 tree peony cultivars, from which a total of 595 amplified fragments were detected, and 377 of them were polymorphic with a polymorphism percentage of 64.3%, the number of amplified fragments and the number of polymorphic bands for each primer combinations was 25.9 and 16.4, respectively.Its genetic similarity coefficient in pairs was between 0.567 and 0.894, the average was 0.752.The 58 tree peony cultivars were grouped into seven clusters at genetic similarity of 0.692.This indicated that the genetic diversity of these cultivars was abundant.Moreover, the clustering results showed that the cluster was not completely consistent with the classification of floral forms though there was a tendency that the single-flower section and the proliferate-flower one were to get together separately.And the cultivars with close color scattered in different categories instead of clustering together.That is to say there is a great genetic difference between the low and high polyphyll cultivars, but there is no clear relationship between the clusters with floral form, color and characteristics.This might be due to the introduction, selection and repeat hybridization of tree peony for a long time, leading to the complicated sources of varieties

Key words: Chinese tree peony, Core collection, SRAP molecular mark, Genetic diversity

中图分类号: 

• S685.03