华北农学报 ›› 2018, Vol. 33 ›› Issue (6): 116-122. doi: 10.7668/hbnxb.2018.06.016

所属专题: 水稻 生物技术

• 论文 • 上一篇    下一篇

粳稻栽培品种中淀粉合成相关基因的等位变异及群体结构分析

张善磊1,2, 岳红亮2, 赵春芳2, 陈涛2, 张亚东2, 周丽慧2, 赵凌2, 梁文化2, 王才林1,2   

  1. 1. 南京农业大学 农学院, 江苏省现代作物生产协同创新中心, 江苏 南京 210095;
    2. 江苏省农业科学院 粮食作物研究所, 江苏省优质水稻工程技术研究中心, 国家水稻改良中心南京分中心, 江苏 南京 210014
  • 收稿日期:2018-07-28 出版日期:2018-12-28
  • 通讯作者: 王才林(1959-),男,江苏无锡人,研究员,博士,博士生导师,主要从事水稻遗传育种研究。
  • 作者简介:张善磊(1990-),男,安徽六安人,在读硕士,主要从事水稻遗传育种研究。
  • 基金资助:
    国家科技支撑计划项目(2015BAD01B02);江苏省重点研发项目(BF2016370);江苏省自主创新资金(SCX(17)1063);现代农业产业技术体系(CARS-01-62)

Allelic Variation of Starch Synthesis Related Genes and Population Structure in Japonica Rice Cultivars

ZHANG Shanlei1,2, YUE Hongliang2, ZHAO Chunfang2, CHEN Tao2, ZHANG Yadong2, ZHOU Lihui2, ZHAO Ling2, LIANG Wenhua2, WANG Cailin1,2   

  1. 1. College of Agriculture, Nanjing Agricultural University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210095, China;
    2. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R & D Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China
  • Received:2018-07-28 Published:2018-12-28

摘要: 为了明确粳稻栽培品种中淀粉合成相关基因的等位变异及群体结构和亲缘关系状况,以国内外181份粳稻栽培品种为试验材料,利用34个淀粉合成相关基因的分子标记进行遗传多样性分析,并结合185个SSR/Indel标记分析其群体结构及亲缘关系。34个淀粉合成相关基因分子标记共检测到74个等位基因,变幅为2~4个,平均每个标记检测到的等位基因数为2.176个,基因多样性指数变幅为0.011~0.494,平均0.148;多态信息含量(PIC)变幅为0.011~0.399,平均0.128,中度多态位点(0.25 < PIC < 0.50)有6个,无高度多态位点(PIC > 0.50),表明粳稻中淀粉合成相关基因的遗传多样性不够丰富。群体结构分析及基于Nei's遗传距离的UPGMA聚类分析表明,供试材料分为3个亚群(POP1~POP3)和1个混合群(Mixed),地理位置相近的品种大都聚于同一亚群中。可为水稻食味品质性状的遗传改良提供依据,为后续淀粉合成相关基因与淀粉品质性状的关联分析奠定基础。

关键词: 粳稻, 遗传多样性, 群体结构, 淀粉合成相关基因

Abstract: To evaluate the allelic variation of starch synthesis related genes in Japonica rice cultivars, their population structure and genetic relationship, 34 molecular markers of starch synthesis related genes were used to detect the genetic diversity of 181 Japonica rice cultivars and their population structure and genetic relationship were analyzed combine with 185 SSR/Indel markers. A total of 74 alleles were detected by 34 molecular markers of starch synthesis related genes, ranging from 2 to 4, with an average value of 2.176 alleles per marker. Genetic diversity index ranged from 0.011 to 0.494 with an average value of 0.148. The polymorphism information content (PIC) ranged from 0.011 to 0.399 with an average value of 0.128. Six moderately polymorphic loci (0.25 < PIC < 0.50) and zero highly polymorphic locus (PIC>0.50) were detected, indicating that the genetic diversity of starch synthesis related genes in Japonica rice cultivars was not enough at present. Population structure analysis and UPGMA cluster analysis based on Nei's genetic distance divided the populations into three main groups (POP1-POP3) and one mixed group (Mixed). Most of the cultivars derived from the neighboring regions were clustered into one large group. It could provide a basis for genetic improvement of eating quality traits of rice, and lay a foundation for the subsequent association analysis of starch synthesis-related genes and starch quality traits.

Key words: Japonica rice, Genetic diversity, Population structure, Starch synthesis related genes

中图分类号: 

引用本文

张善磊, 岳红亮, 赵春芳, 陈涛, 张亚东, 周丽慧, 赵凌, 梁文化, 王才林. 粳稻栽培品种中淀粉合成相关基因的等位变异及群体结构分析[J]. 华北农学报, 2018, 33(6): 116-122. doi: 10.7668/hbnxb.2018.06.016.

ZHANG Shanlei, YUE Hongliang, ZHAO Chunfang, CHEN Tao, ZHANG Yadong, ZHOU Lihui, ZHAO Ling, LIANG Wenhua, WANG Cailin. Allelic Variation of Starch Synthesis Related Genes and Population Structure in Japonica Rice Cultivars[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(6): 116-122. doi: 10.7668/hbnxb.2018.06.016.

使用本文

0
    /   /   推荐 /   导出引用

链接本文: http://www.hbnxb.net/CN/10.7668/hbnxb.2018.06.016

               http://www.hbnxb.net/CN/Y2018/V33/I6/116