<em>MyoG</em>基因多态性与牦牛生长性能的关联分析

doi:10.7668/hbnxb.2018.06.015

华北农学报 ›› 2018, Vol. 33 ›› Issue (6): 108-115. doi: 10.7668/hbnxb.2018.06.015

所属专题： 畜牧；生物技术；

MyoG基因多态性与牦牛生长性能的关联分析

柴志欣^1,2, 信金伟³, 王会^1,2, 郭琳^1,2, 钟金城^1,2, 张成福³, 姬秋梅³

1. 西南民族大学青藏高原研究院, 四川成都 610041;
2. 青藏高原动物遗传资源保护与利用四川省、教育部重点实验室, 四川成都 610041;
3. 西藏自治区农牧科学院, 省部共建青稞和牦牛种质资源与遗传改良国家重点实验室, 西藏拉萨 850009

收稿日期:2018-09-22 出版日期:2018-12-28
通讯作者: 钟金城(1963-),男,云南绿春人,教授,博士,主要从事动物遗传学研究。
作者简介:柴志欣(1984-),女,山西大同人,讲师,硕士,主要从事牦牛遗传育种研究。
基金资助:
中央高校基本科研业务费专项基金项目（2016NZYQN30）；国家肉牛牦牛产业技术体系项目（CARS-37）

Polymorphisms of MyoG Gene and Analysis of Relevance with Growth Performances in Yak

CHAI Zhixin^1,2, XIN Jinwei³, WANG Hui^1,2, GUO Lin^1,2, ZHONG Jincheng^1,2, ZHANG Chengfu³, JI Qiumei³

1. Institute of Qinghai-Tibet Plateau, Southwest Minzu University, Chengdu 610041, China;
2. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Sichuan Province and Ministry of Education, Chengdu 610041, China;
3. Tibet Academy of Agricultural and Animal Husbandry Sciences, State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Lhasa 850009, China

Received:2018-09-22 Published:2018-12-28

摘要/Abstract

摘要： 旨在对西藏主要牦牛类群及四川麦洼牦牛生长性状指标进行比较分析，并选取MyoG基因进行SNPs检测，研究其与牦牛体尺性状的相关性，为有效评估西藏主要牦牛类群的生长性能提供参考。采用DNA池直接测序法进行候选基因全区段遗传变异检测，采用SPSS 17.0程序中最小二乘线性模型对MyoG基因多态位点基因型与牦牛体质量、体尺性状进行关联分析。结果显示，申扎牦牛生长指标明显小于麦洼牦牛、类乌齐牦牛和帕里牦牛，而类乌齐牦牛各性状指标均较优，不同生态环境及气候条件直接影响草场、植被类型及其长势情况，进而影响牦牛的采食量和生长发育。牦牛MyoG基因共检测到g.757 T > C、g.662 G > A、g.539 A > G和g.2216 A > G共4个突变位点，均包含3种基因型，且符合Hardy-Weinberg平衡，其中，g.757 T > C、g.662 G > A和g.539 A > G位点在申扎和帕里牦牛中多态性较高，在其他群体中较低。差异显著性检验表明，g.757 T > C、g.662 G > A和g.539 A > G与体高显著相关（P<0.05）。MyoG基因可能是影响牦牛体高性状的主基因或与主基因相连锁的基因座，可作为辅助选择的遗传标记。

关键词: 牦牛, MyoG基因, 基因多态性, 生长性状, 相关分析

Abstract: The aims of this study were to compare the characteristics of maiwa yak in Sichuan, leiwuqi yak, shenzhayak, pali yak, sibu yak in Tibet, select the MyoG gene to detection of SNPs, to reveal the association between different genotypes and growth traits. This study provided a reference for the effective evaluation of the growth performance to the main yak population in Tibet. The DNA pool technology and direct sequencing were adopted to study in the genetic variation of the candidate genes. GLM statistical model of SPSS 17.0 software was used to analyze the association with different genotypes corresponding to the SNPs MyoG with growth traits, such as body height, body length, chest circumference, cannon circumference and body weight. The results showed that the growth indexes of shenzha yak were significantly lower than maiwa yak, leiwuqi yak and pali yak, but the traits of leiwuqi yak were better. Different ecological environments and climatic conditions directed affect grassland, vegetation types and their growth conditions, which were affect the feed intake and growth of yak. Four SNPs were found in MyoG gene (g.757 T>C,g.662 G>A,g. 539 A>G and g. 2216 A>G). There were three genotypes. The fitness test showed that MyoG gene on polymorphic loci in Hardy-Weinberg equilibrium state. The polymorphism of g.757 T>C,g.662 G>A and g. 539 A>G was higher in shenzha yak and pali yak, and lower in other groups. The different significance test indicated that the g.757 T>C,g.662 G>A and g. 539 A>G of the MyoG gene were significantly related to the body height(P<0.05). There were the major effect genes influencing the body height of the yak or loci might be closely linked to the major effect gene and they could be regarded as the genetic markers for the assistant selection.

Key words: Yak, MyoG gene, Polymorphism, Growth traits, Association analysis

中图分类号:

S823
S813.3

柴志欣, 信金伟, 王会, 郭琳, 钟金城, 张成福, 姬秋梅. MyoG基因多态性与牦牛生长性能的关联分析[J]. 华北农学报, 2018, 33(6): 108-115. doi: 10.7668/hbnxb.2018.06.015.

CHAI Zhixin, XIN Jinwei, WANG Hui, GUO Lin, ZHONG Jincheng, ZHANG Chengfu, JI Qiumei. Polymorphisms of MyoG Gene and Analysis of Relevance with Growth Performances in Yak[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(6): 108-115. doi: 10.7668/hbnxb.2018.06.015.

http://www.hbnxb.net/CN/Y2018/V33/I6/108

参考文献

[1] Wright W E, Sassoon D A, Lin V K. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD[J]. Cell, 1989, 56(4):607-617.
[2] Bergstrom D A, Tapscott S J. Molecular distinction between specification and differentiation in the myogenic basic helix-loop-helix transcription factor family[J]. Molecular and Cellular Biology, 2001, 21(7):2404-2412.
[3] 赵青, 钟土木, 徐宁迎. 金华猪MyoG基因多态性与部分生长性能的关系[J]. 华南农业大学学报, 2010, 31(2):121-124.
[4] Te Pas M F W, Soumillion A, Harders F L, et al. Influences of myogenin genotypes on birth weight, growth rate, carcass weight, backfat thickness, and lean weight of pigs[J]. Journal of Animal Science, 1999, 77(9):2352-2356.
[5] Neville C M, Schmidt M, Schmidt J. Response of myogenic determination factors to cessation and resumption of electrical activity in skeletal muscle:a possible role for myogenin in denervation supersensitivity[J]. Cellular and Molecular Neurobiology, 1992, 12(6):511-527.
[6] Weintraub H, Davis R, Tapscott S, et al. The myoD gene family:nodal point during specification of the muscle cell lineage[J]. Science, 1991, 251(4995):761-766.
[7] Hasty P, Bradley A, Morris J H, et al. Muscle deficiency and neonatal death in mice with a targeted mutation in the myogenin gene[J]. Nature, 1993, 364(6437):501-506.
[8] Fujisawa-Sehara A. Development and regeneration of skeletal muscle[J]. Tanpakushitsu Kakusan koso. Protein, Nucleic Acid, Enzyme, 2000, 45(13 S1):2228-2234.
[9] Buonanno A, Edmondson D G, Hayes W P. Upstream sequences of the myogenin gene convey responsiveness to skeletal muscle denervation in transgenic mice[J]. Nucleic Acids Research, 1993, 21(24):5684-5693.
[10] 王永智.牛的外貌鉴定、体尺测量及体重估测[J]. 养殖技术顾问, 2013(12):19-20.
[11] 李强, 傅昌秀, 文勇立, 等. 金川多肋牦牛体尺和屠宰性状测定及其相关性分析[J]. 中国草食动物科学, 2012, 32(2):19-20.
[12] 徐怀超, 昝林森, 王洪宝, 等. CRTC3基因多态性及基因型组合与秦川牛生长性状的关联分析[J]. 畜牧兽医学报, 2016, 47(11):2184-2190.
[13] 王晓军, 程绍敏. 西藏主要气候特征分析[J]. 高原山地气象研究, 2009, 29(4):81-84.
[14] 邹联付, 潘刚, 方江平. 西藏三江流域生态功能区划研究[J]. 西藏科技, 2009(5):18-21.
[15] 姬秋梅, 达娃央拉, 洛桑, 等. 西藏帕里牦牛本品种选育研究[J]. 西藏科技, 2011(2):60-62.
[16] 栾兆进,贺建宁,程明,等.绵羊肌肉组织MyoG和PID1基因的表达及其与肌内脂肪含量的关系[J].畜牧兽医学报, 2016, 47(10):1986-1994.
[17] Liu Y Y, Li F N, Kong X F, et al. Signaling pathways related to protein synthesis and amino acid concentration in pig skeletal muscles depend on the dietary protein level, genotype and developmental stages[J]. PLoS One, 2015, 10(9):1-21.
[18] 赵忠海, 李辉, 易恒洁, 等. MyoG和MEF2a基因多态性聚合效应对鸭屠宰性状的影响[J]. 中国农业科学, 2016, 49(18):3649-3661.
[19] 杨又兵. 猪MYOG基因和CAST基因多态性研究及部分DNA片段的测序[D]. 武汉:华中农业大学, 2003.
[20] 朱超杰, 张立春, 秦立红, 等. 不同品种牛MyoG基因Exon 1多态性比较分析[J]. 中国兽医学报, 2016, 36(6):1024-1031.
[21] 薛梅, 昝林森, 王洪宝, 等. 6个黄牛群体MyoG基因单核苷酸多态性及其与体尺性状的相关性[J]. 西北农林科技大学学报, 2011, 39(7):35-42.
[22] 韩银仓, 孙永刚, 陈志. MyoG基因SNP检测及与藏羊体尺性状的关联分析[J]. 家畜生态学报, 2016, 37(6):19-23.
[23] 白俊艳, 杨又兵, 王玉琴, 等. 绵羊MyoG基因外显子1的多态性及其与肉质性状的关联性分析[J]. 中国畜牧兽医, 2017, 44(6):1778-1783.
[24] 林亚秋, 张倡珲, 郑玉才, 等. 九龙牦牛肌细胞生成素基因的克隆及其表达谱[J]. 畜牧兽医学报, 2014, 45(2):207-211.
[25] 王敏强, 苏培, 刘晓玲, 等. 黄牛,牦牛和水牛MyoG基因单核苷酸多态性分析[J]. 西北农业学报, 2011, 20(6):7-11.
[26] Bhuiyan M S A, Kim N K, Choyoon Y M, et al. Identification of SNPs in MyoG gene family and their associations with carcass traits in cattle[J]. Livestock Science, 2009,126:292-297.

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