牦牛<i>KDM7A</i>表达谱分析及其在卵母细胞减数分裂进程中的表达

doi:10.7668/hbnxb.20191191

摘要/Abstract

摘要： 为探究组蛋白去甲基化酶7A（KDM7A）在牦牛生殖发育过程中的作用机制及表达模式，以牦牛作为研究对象，通过RT-PCR技术克隆获得牦牛KDM7A编码区序列，结合相关生物信息学分析软件分析KDM7A蛋白的结构和功能，利用实时荧光定量PCR（RT-PCR）获得KDM7A mRNA在牦牛卵巢、肾、胃、小肠、肌肉、心、子宫、脾、肺和肝中的表达水平，并分析该基因在卵母细胞成熟过程中的表达规律。结果显示，克隆出牦牛2 704 bp的KDM7A基因，其中CDS区全长为2 409 bp，共编码802个氨基酸。牦牛与其他物种中黄牛、野牛及山羊同源性较高；KDM7A在牦牛各组织中广谱表达，其在子宫中的相对表达量最高，显著高于除胃以外其他组织（P<0.05），而在肾脏和肌肉组织中相对表达量较低，显著低于其他组织（P<0.05）；在卵母细胞减数分裂过程中，KDM7A基因具有时空动态表达特征，减数第二次分裂中期相对表达量显著高于减数第一次分裂中期和卵母细胞生发泡期（P<0.05）。综上表明，KDM7A在遗传进化过程中高度保守，在牦牛卵母细胞减数分裂进程中具有时序性表达模式，提示KDM7A参与卵母细胞的减数分裂进程，为深入探究牦牛KDM7A在生殖过程中的作用机制提供基础依据。

关键词: 牦牛, 组蛋白去甲基化酶, KDM7A, 卵母细胞, 组织表达谱

Abstract: The objective of this research was to explore the expression pattern and molecular mechanism of histone demethylase 7A(KDM7A) in the reproductive development of yak.Yak was used as research object. The coding sequence of KDM7A gene was cloned by RT-PCR. The protein structure and function of KDM7A were analyzed by bioinformatics software, and the KDM7A gene was detected by Real-time quantitative PCR (RT-qPCR)in theovary, kidney,stomach,small intestine,muscle, heart, uterus, spleen, lung and liver tissue. The expression pattern of KDM7A mRNA was analyzed during the meiotic maturation of yak oocytes.The 2 704 bp KDM7A gene of the yak was cloned, and the CDS was 2 409 bp in length and encoded a total of 802 amino acids. Compared with other species, the base sequence of yak KDM7A was higher homology with cattle, bison and goat. Yak KDM7A gene was widely expressed in various tissues,in which the uterus KDM7A mRNA relative expression was the highest and significantly higher than other tissues except the stomach (P<0.05), while the relative expression of KDM7A in kidney and muscle tissue was significantly lower than that in other tissues (P<0.05).There was spatiotemporal dynamic expression feature of the KDM7A gene during the meiosis of oocytes. The expression level of KDM7A in meiosis metaphase Ⅱ (MⅡ) stage was significantly higher than that in meiosis metaphase Ⅰ(MⅠ) and germinal vesicle (GV) stages of yak oocytes (P<0.05).In summary, this study suggested that KDM7A gene was highly conserved in genetic evolution and involved in meiosis process of oocytes, which had a dynamic expression pattern during the reproductive development of yak. This study provided relevant basis for further exploring the action mechanism of yak KDM7A in the process of reproduction.

Key words: Yak, Histone demethylase, KDM7A, Oocyte, Tissue expression profile

中图分类号:

S823.8+5

海卓, 熊显荣, 马鸿程, 黄向月, 闵星宇, 李键. 牦牛KDM7A表达谱分析及其在卵母细胞减数分裂进程中的表达[J]. 华北农学报, 2020, 35(6): 217-224. doi: 10.7668/hbnxb.20191191.

HAI Zhuo, XIONG Xianrong, MA Hongcheng, HUANG Xiangyue, MIN Xingyu, LI Jian. Analysis of KDM7A Expression Profile in Yak and Its Expression in the Process of Oocyte Meiosis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 217-224. doi: 10.7668/hbnxb.20191191.

http://www.hbnxb.net/CN/Y2020/V35/I6/217

参考文献

[1] 秦文昌,殷实,李泽沛,王斌,杨柳青,周婧雯,李键.牦牛HDAC8 基因特征及其在组织和卵母细胞成熟过程中的表达分析[J].华北农学报,2020,35(2):210-216.doi:10.7668/hbnxb.20190532.
Qin W C, Yin S, Li Z P,Wang B, Yang L Q, Zhou J W, Li J. The characteristic of yak HDAC8 gene and analysis of their expression in different tissues and during the oocyte maturation[J]. Acta Agriculturae Boreali-Sinica,2020, 35(2):210-216.
[2] Marcho C,Oluwayiose O A,Pilsner J R. The preconception environment and sperm epigenetics[J]. Andrology,2020,8(4):924-942.doi:10.1111/andr.12753.
[3] Li X G. Epigenetics and cell cycle regulation in cystogenesis[J]. Cellular Signalling,2020,68:109509.doi:10.1016/j.cellsig.2019.109509.
[4] 肖婷,周菊,傅俊江. 组蛋白修饰作为表观遗传肿瘤标志物的研究进展[J].西南医科大学学报,2019,42(3):284-288.doi:10.3969/j.issn.2096-3351.2019.03.018.
Xiao T,Zhou J,Fu J J. Research progress in histone modifications as epigenetic tumor markers[J]. Journal of Southwest Medical University,2019,42(3):284-288.
[5] 魏猛. 组蛋白去甲基化酶KDM7在肝细胞癌中的表达及其临床意义[D]. 济南:山东大学,2014.
Wei M. Expression of KDM7 enzymes in human hepatocellular carcinoma and its clinical significance[D].Jinan:Shandong University,2014.
[6] Reid M A,Dai Z W,Locasale J W. The impact of cellular metabolism on chromatin dynamics and epigenetics[J]. Nature Cell Biology, 2017,19(11):1298-1306.doi:10.1038/ncb3629.
[7] Fellous A,Earley R L,Silvestre F. The Kdm/Kmt gene families in the self-fertilizing mangrove rivulus fish, Kryptolebias marmoratus,suggest involvement of histone methylation machinery in development and reproduction[J]. Gene,2019,687:173-187.doi:10.1016/j.gene.2018.11.046.
[8] Horton J R,Upadhyay A K,Qi H H,Zhang X,Shi Y,Cheng X D. Enzymatic and structural insights for substrate specificity of a family of jumonji histone lysine demethylases[J]. Nature Structural & Molecular Biology,2010,17(1):38-43.doi:10.1038/nsmb.1753.
[9] Yue W W,Hozjan V,Ge W,Loenarz C,Cooper C D O,Schofield C J,Kavanagha K L,Oppermann U,McDonoughc M A. Crystal structure of the PHF8 jumonji domain, an N^ε-methyl lysine demethylase[J]. Febs Letters,2010,584(4):825-830.doi:10.1016/j.febslet.2009.12.055.
[10] Huang C Y,Xiang Y,Wang Y R,Li X,Xu L Y,Zhu Z Q,Zhang T,Zhu Q Q,Zhang K J, Jing N H,Chen C L. Dual-specificity histone demethylase KIAA1718(KDM7A) regulates neural differentiation through FGF4[J]. Cell Research,2010,20(2):154-165.doi:10.1038/cr.2010.5.
[11] Mallm J P,Windisch P,Biran A,Gal Z,Schumacher S,Glass R,Herold-Mende C,Meshorer E,Barbus M,Rippe K. Glioblastoma initiating cells are sensitive to histone demethylase inhibition due to epigenetic deregulation[J]. Internitional Journal of Cancer,2020,146(5):1281-1292.doi:10.1002/ijc.32649.
[12] Meng Z Z,Liu Y, Wang J,Fan H J,Fang H,Li S,Yuan L,Liu C C,Peng Y,Zhao W W,Wang L L,Li J,Feng J. Histone demethylase KDM7A is required for stem cell maintenance and apoptosis inhibition in breast cancer[J]. Journal of Cellular Physiology,2020,235(2):932-943.doi:10.1002/jcp.29008.
[13] Huang C Y,Chen J,Zhang T,Zhu Q Q,Xiang Y,Chen C D,Jing N H. The dual histone demethylase KDM7A promotes neural induction in early chick embryos[J]. Develomental Dynamics,2010,239(12):3350-3357.doi:10.1002/dvdy.22465.
[14] Kujirai T,Kurumizaka H. Transcription through the nucleosome[J]. Current Opinion in Structural Biology, 2020,61:42-49.doi:10.1016/j.sbi.2019.10.007.
[15] 杨勤,柴志欣,王吉坤,王会,钟金城. 牦牛ACTA1基因启动子区克隆、DNA甲基化与组织表达相关性分析[J]. 华北农学报,2020,35(1):222-229.doi:10.7668/hbnxb.20190124.
Yang Q,Chai Z X,Wang J K,Wang H,Zhong J C. Cloning of ACTA1 gene promoter region and correlation analysis between DNA methylation and tissue expression in yak[J]. Acta Agriculturae Boreali-Sinica,2020,35(1):222-229.
[16] Thakali K M,Zhong Y,Cleves M,Andres A,Shankar K. Associations between maternal body mass index and diet composition with placental DNA methylation at term[J]. Placenta,2020,93:74-82.doi:10.1016/j.placenta.2020.02.018.
[17] Bird A. DNA methylation:mega-year inheritance with the help of darwin[J]. Current Biology, 2020,30(7):319-321.doi:10.1016/j.cub.2020.02.034.
[18] Trojer P,Reinberg D. Histone lysine demethylases and their impact on epigenetics[J]. Cell,2006,125(2):213-217.doi:10.1016/j.cell.2006.04.003.
[19] Yang W N,Jiang D H,Jiang J F, He Y H. A plant-specific histone H3 lysine 4 demethylase represses the floral transition in Arabidopsis[J]. The Plant Journal,2010,62(4):663-673.doi:10.1111/j.1365-313X.2010.04182.x.
[20] Jeong J H,Song H R,Ko J H,Jeong Y M,Kwon Y E,Seol J H,Amasino R M,Noh B,Noh Y S. Repression of flowering locust chromatin by functionally redundant histone H3 lysine 4 demethylases in Arabidopsis[J]. PLoS One,2009,4(11):e8033.doi:10.1371/journal.pone.0008033.
[21] Lin C H,Li B,Swanson S,Zhang Y,Florens L,Washburn M P,Abmayr S M,Workman J L. Heterochromatin protein 1a stimulates histone H3 lysine 36 demethylation by the Drosophila KDM4A demethylase[J]. Molecular Cell,2008,32(5):696-706.doi:10.1016/j.molcel.2008.11.008.
[22] Sánchez-fernández E M,Tarhonskaya H,Al-Qahtani K,Hopkinson R J,Mccullagh J S O,Schofield C J,Flashman E. Investigations on the oxygen dependence of a 2-oxoglutarate histone demethylase[J]. Biochemical Journal,2013,449(2):491-496.doi:10.1042/BJ20121155.
[23] Chen H B,Costa M. Iron-and 2-oxoglutarate-dependent dioxygenases:an emerging group of molecular targets for nickel toxicity and carcinogenicity[J]. BioMetals,2009,22(1):191-196.doi:10.1007/s10534-008-9190-3.
[24] Kim J,Singh A K,Takata Y,Lin K,Shen J J,Lu Y,Kerenyi M A,Orkin S H,Chen T P. LSD1 is essential for oocyte meiotic progression by regulating CDC25B expression in mice[J]. Nature Communications,2015,6(6):10116.doi:10.1038/ncomms10116.
[25] Kawakami E,Tokunaga A,Ozawa M,Sakamoto R,Yoshida N. The histone demethylase Fbxl11/Kdm2a plays an essential role in embryonic development by repressing cell-cycle regulators[J]. Mechanisms of Development,2015,135(135):31-42.doi:10.1016/j.mod.2014.10.001.
[26] Rissi V B,Glanzner W G,De Macedo M P,Gutierrez K,Baldassarre H,Gonçalves P B D,Bordignon V. The histone lysine demethylase KDM7A is required for normal development and first cell lineage specification in porcine embryos[J]. Epigenetics,2019,14(11):1088-1101.doi:10.1080/15592294.2019.1633864.
[27] 杨显英,熊显荣,蔡雯祎,韩杰,阿果约达,李键.牦牛KDM2A 基因克隆及其在不同组织和减数分裂进程的表达[J].农业生物技术学报,2018,26(7):1186-1194.doi:10.3969/j.issn.1674-7968.2018.07.010.
Yang X Y,Xiong X R,Cai W Y,Han J,Aguo Y D,Li J. Cloning of yak (Bos grunniens) KDM2A gene and its expression in different tissues and mitotic progression[J]. Journal of Agricultural Biotechnology,2018,26(7):1189-1194.
[28] Meng Z Z,Liu Y,Wang J,Fan H J,Fang H,Li S,Yuan L,Liu C C,Peng Y,Zhao W W,Wang L L,Li J,Fang J. Histone demethylase KDM7A is required for stem cell maintenance and apoptosis inhibition in breast cancer[J]. Journal of Cellular Physiology,2020,235(2):932-943.doi:10.1002/jcp.29008.
[29] Marinho L S R,Rissi V B,Lindquist A G,Seneda M M,Bordignon V. Acetylation and methylation profiles of H3K27 in porcine embryos cultured in vitro[J]. Zygote,2017,25(5):575-582.doi:10.1017/S0967199417000405.
[30] 王帅. 牛HDM家族成员在卵母细胞中的表达规律及JMJD1C在早期胚胎发育中的功能[D]. 长春:吉林大学,2014.
Wang S. Expression pattern of HDM family members in bovine oocytes and function of JMJD1C in early embryo development[D]. Changchun:Jilin University,2014.

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牦牛KDM7A表达谱分析及其在卵母细胞减数分裂进程中的表达

Analysis of KDM7A Expression Profile in Yak and Its Expression in the Process of Oocyte Meiosis

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牦牛KDM7A表达谱分析及其在卵母细胞减数分裂进程中的表达

Analysis of KDM7A Expression Profile in Yak and Its Expression in the Process of Oocyte Meiosis

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