华北农学报 ›› 2018, Vol. 33 ›› Issue (4): 120-125. doi: 10.7668/hbnxb.2018.04.017

所属专题: 油料作物

• 作物遗传育种·种质资源·生物技术 • 上一篇    下一篇

基于SSR标记的杂交大豆主要亲本遗传多样性分析

白志元1, 张瑞军1, 武国平2, 连世超1, 杨玉花1, 卫保国1   

  1. 1. 山西省农业科学院 农作物品种资源研究所, 农业部黄土高原作物基因资源与种质创制重点实验室, 杂粮种质资源发掘与遗传改良山西省重点实验室, 山西 太原 030031;
    2. 山西省农业科学院 园艺研究所, 山西 太原 030031
  • 收稿日期:2018-04-01 出版日期:2018-08-28
  • 通讯作者: 张瑞军(1972-),男,山西河曲人,副研究员,硕士,主要从事大豆杂种优势利用研究。
  • 作者简介:白志元(1985-),男,山西五台人,助理研究员,硕士,主要从事大豆杂种优势利用研究。
  • 基金资助:
    国家重点研发计划项目(2016YFD0101500;2016YFD0101504);山西省重点研发计划项目(201603D21101);山西省农业科学院重点攻关项目(YGG1650);山西省农业科学院生物育种工程项目(17YZGC102)

Genetic Diversity Analysis of Main Parents for Hybrid Soybean Based on SSR Markers

BAI Zhiyuan1, ZHANG Ruijun1, WU Guoping2, LIAN Shichao1, YANG Yuhua1, WEI Baoguo1   

  1. 1. Institute of Crop Germplasm Resources, Shanxi Academy of Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan 030031, China;
    2. Gardening Research Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China
  • Received:2018-04-01 Published:2018-08-28

摘要: 为明确山西已有杂交大豆主要亲本的遗传多样性和亲缘关系,利用64对SSR引物对32份杂交大豆亲本包括15份保持系与17份恢复系进行了遗传多样性分析。结果共检测到357个等位基因变异,单个位点检测到的等位基因数变化为2~12个,平均为5.578个,其中,保持系检测到322个等位基因变异,变化为2~9个,平均为5.031个,恢复系检测到320个等位基因变异,变化为2 ~ 12个,平均为5个;总的位点多态性信息含量(PIC)变辐为0.368~0.900,平均为0.666,其中,保持系PIC变辐为0.370~0.868,平均为0.661,恢复系PIC变辐为0.329~0.904,平均为0.630。聚类分析结果表明,保持系间的遗传相似系数(GS)平均为0.637,在相似系数为0.61处,可将供试的保持系聚为两大类;其中,第Ⅱ类在相似系数为0.65处又可分为3个亚类;恢复系间的遗传相似系数平均为0.669,在相似系数为0.65处,将供试的恢复系聚为两大类;聚类结果与品种地理来源具有一定的相关性。

关键词: 大豆, 遗传多样性, SSR标记

Abstract: To determine the genetic diversity and genetic relationship of the main parents of hybrid soybean in Shanxi Province, 64 pairs of SSR primers were used to analyze the genetic diversity of 32 hybrid soybean parents including 15 maintainer lines and 17 restorer lines. Totally 357 alleles were revealed among 32 parents, 2-12 alleles could be detected at different SSR locus, with the average of 5.578 alleles per SSR marker, of which 322 alleles were detected in the maintainer line, ranging from 2 to 9 with an average of 5.031, and the detection of 320 alleles in the restorer ranged from 2 to 12 with an average of 5. The polymorphism information content (PIC)of the total polymorphism was 0.368-0.900, with an average of 0.666, of which, the PIC variation of maintainer lines ranged from 0.370 to 0.868 with an average of 0.661, and the PIC variation of restorer lines ranged from 0.329 to 0.904 with an average of 0.630. Cluster analysis result showed that the average genetic similarity coefficient (GS)between maintainer lines was 0.637. At the genetic similarity coefficient (GS)of 0.61, the maintainer lines could be grouped into two major groups. At the similarity coefficient of 0.65, the second group could be divided into three subcategories. The average genetic similarity coefficient (GS)between restorer lines was 0.669. At the genetic similarity coefficient (GS)of 0.65, the restorer lines could be grouped into two major groups. The clustering results were related to the geographical origin of cultivars.

Key words: Soybean, Genetic diversity, SSR marker

中图分类号: 

引用本文

白志元, 张瑞军, 武国平, 连世超, 杨玉花, 卫保国. 基于SSR标记的杂交大豆主要亲本遗传多样性分析[J]. 华北农学报, 2018, 33(4): 120-125. doi: 10.7668/hbnxb.2018.04.017.

BAI Zhiyuan, ZHANG Ruijun, WU Guoping, LIAN Shichao, YANG Yuhua, WEI Baoguo. Genetic Diversity Analysis of Main Parents for Hybrid Soybean Based on SSR Markers[J]. Acta Agriculturae Boreali-Sinica, 2018, 33(4): 120-125. doi: 10.7668/hbnxb.2018.04.017.

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