华北农学报 ›› 2007, Vol. 22 ›› Issue (1): 108-111. doi: 10.3321/j.issn:1000-7091.2007.01.025

所属专题: 畜牧

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不同品种绵羊杂交后代RAPD的分析

李天俊1, 王英超1, 张坤婷1, 潘淑英1, 宋桂敏1, 王通1, 李振国2, 刘炳才2, 李秀红2   

  1. 1. 天津农学院, 天津 300384;
    2. 天津市大港区农林畜牧局, 天津 300384
  • 收稿日期:2006-03-01 出版日期:2007-02-28
  • 通讯作者: 宋桂敏(1959-),女,天津人,硕士,教授,主要从事动物育种学研究。
  • 作者简介:李天俊(1965-),女,天津人,硕士,高级实验师,主要从事生物化学实验教学工作。
  • 基金资助:
    天津市科技攻关培育项目(032127611)

The Analyse of Different Sheep Cross Breed by Using RAPD

LI Tian-jun1, WANG Ying-chao1, ZHANG Kun-ting1, PAN Shu-ying1, SONG Gui-min1, WANG Tong1, LI Zhen-guo2, LIU Bing-cai2, LI Xiu-hong2   

  1. 1. Tianjin Agricultral College, Tianjin 300384, China;
    2. Tianjin Dagang District Furming Bureau, Tianjin 300384, China
  • Received:2006-03-01 Published:2007-02-28

摘要: 对3个不同绵羊类群(无角道塞特二代公羊、杜泊一代公羊、杜泊二代公羊)共8个样本应用20个随机引物扩增进行RAPD分析。结果表明,在20个随机引物中,有7个随机引物扩增出不同程度的清晰可辨的RAPD谱带,其中标记总数为35条,多态标记数为24条。共得到147条清晰的谱带。杜泊一代与杜泊二代2个种群间遗传距离最小,为0.0809;杜泊一代与无角道塞特2个种群间遗传距离最大,为0.5152.

关键词: 随机扩增多态DNA, 绵羊, 遗传距离

Abstract: The genetic relationship of 3 isolates of Poll Dorset F2, Dorper F1, Dorper F2 which are 8 samples was examined using 20 primers by random amplified polymorphic DNA (RAPD). The result indicated that in 20 primers, 7 primers clearly amplified the varying bands which is easy to distinguish. We altogether get 147 clear bands. the mark total is 35 and the polymorphic mark is 24. The genetic distances between Dorper F1 and Dorper F2 is the smallest, is (0.0809), while the genetic distances between Poll Dorset F2 and Dorper F1 are biggest, is 0.5152.

Key words: RAPD(Randomly Amplified Polymorphic DNA), Sheep breeds, Genetic distance

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引用本文

李天俊, 王英超, 张坤婷, 潘淑英, 宋桂敏, 王通, 李振国, 刘炳才, 李秀红. 不同品种绵羊杂交后代RAPD的分析[J]. 华北农学报, 2007, 22(1): 108-111. doi: 10.3321/j.issn:1000-7091.2007.01.025.

LI Tian-jun, WANG Ying-chao, ZHANG Kun-ting, PAN Shu-ying, SONG Gui-min, WANG Tong, LI Zhen-guo, LIU Bing-cai, LI Xiu-hong. The Analyse of Different Sheep Cross Breed by Using RAPD[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(1): 108-111. doi: 10.3321/j.issn:1000-7091.2007.01.025.

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