AY 9944-A-7对于IBMX抑制的绵羊卵母细胞体外成熟的诱导作用

doi:10.7668/hbnxb.2014.06.014

摘要/Abstract

摘要： 为了探究AY 9944-A-7在3-异丁基-1-甲基黄嘌呤(3-isobutyl-1-methylxanthine,IBMX)阻滞的绵羊卵母细胞减数分裂恢复过程中的作用,将绵羊卵丘-卵母细胞复合体(COCs)、分离的卵丘-卵母细胞(split COCs)和脱卵丘后制成的裸卵(DOs)培养于含250 μmol/L IBMX的体外成熟液中,分别添加10,20,30,40 μmol/L的AY 9944-A-7以诱导卵母细胞恢复减数分裂。结果表明,10~40 μmol/L的AY 9944-A-7极显著地提高了绵羊COCs的生发泡破裂(GVBD)率和第一极体(PBI)排出率(P<0.01);添加AY 9944-A-7对IBMX阻滞的绵羊DOs 生发泡破裂和PBI排出没有显著的刺激作用(P>0.05);40 μmol/L AY 9944-A-7使绵羊COCs、split COCs、DOs的退化率极显著提高(P<0.01)。说明AY 9944-A-7能够诱导IBMX抑制的绵羊COCs恢复减数分裂成熟,但是过量的AY 9944-A-7对绵羊卵母细胞有毒性,AY 9944-A-7诱导绵羊COCs克服IBMX抑制恢复减数分裂成熟的适宜剂量为20 μmol/L。

关键词: 绵羊, 卵母细胞, AY 9944-A-7, 3-异丁基-1-甲基黄嘌呤(IBMX)

Abstract: To evaluate the effects of AY 9944-A-7 on 3-isobutyl-1-methylxanthine (IBMX)-arrested meiotic maturation in ovine oocytes,sheep cumulus oocyte complexes (COCs),split COCs and denuded oocytes (DOs) were cultured in medium supplemented with 250 μmol/L IBMX.Different concentrations of AY 9944-A-7 (0,10,20,30,40 μmol/L) were added into maturation medium respectively to induce meiotic resumption of oocytes.The results showed that,AY 9944-A-7 supplementation in the range from 10 to 40 μmol/L increased the percentage of GVBD and the rate of PBI extrusion significantly in ovine COCs (P<0.01).While AY 9944-A-7 showed no significantly stimulatory effect on the percentage of GVBD and the rate of PBI extrusion of ovine DOs arrested by IBMX (P>0.05).And the highest dose tested (40 μmol/L) resulted in significant degenerations in ovine COCs,split COCs and DOs (P<0.01).The results indicated that AY 9944-A-7 induced meiotic resumption inhibited by IBMX in sheep COCs.But AY 9944-A-7 over dose showed toxic effects on ovine oocytes.The optimal dosage for AY 9944-A-7 to overcome the meiotic inhibition by IBMX and resume meiosis was 20 μmol/L.

Key words: Sheep, Oocyte, AY 9944-A-7, 3-isobutyl-1-methylxanthine (IBMX)

中图分类号:

S813.1

郝瑞荣, 吕丽华, 高俊杰, 张春香. AY 9944-A-7对于IBMX抑制的绵羊卵母细胞体外成熟的诱导作用[J]. 华北农学报, 2014, 29(6): 73-77. doi: 10.7668/hbnxb.2014.06.014.

HAO Rui-rong, LÜ Li-hua, GAO Jun-jie, ZHANG Chun-xiang. Roles of AY 9944-A-7 in Inducing in Vitro Maturation of Sheep Oocytes Inhibited by IBMX[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(6): 73-77. doi: 10.7668/hbnxb.2014.06.014.

http://www.hbnxb.net/CN/Y2014/V29/I6/73

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