摘要： 为了观察不同剂量食欲素A在不同时间对绵羊黄体化颗粒细胞OX1R表达的影响,在绵羊黄体化颗粒细胞中添加不同剂量(0.05,0.2,0.5,1μg/mL)食欲素A,分别于刺激后0,2,6,12 h提取细胞总RNA,运用RT-qPCR方法检测了OX1R mRNA相对表达量变化。结果表明,OX1R mRNA分布于绵羊黄体化颗粒细胞中;添加高剂量(1μg/mL)食欲素A在2 h显著性促进OX1R mRNA表达(P<0.01),随后这种作用会逐渐减弱;添加低剂量(0.05,0.2,0.5μg/mL)食欲素A后12 h时会显著促进OX1R mRNA表达(P<0.01),且0.2μg/mL剂量组促进作用更为显著。食欲素A诱导并上调OX1R mRNA的表达,高剂量(1μg/mL)食欲素A可以迅速诱导其表达,而低剂量(0.05,0.2,0.5μg/mL)食欲素A诱导作用较为缓慢,且高剂量(1μg/mL)组食欲素A同低剂量(0.05,0.2,0.5μg/mL)组相比,上调作用更加迅速。

关键词: 食欲素A, 食欲素受体1, 绵羊, 黄体化颗粒细胞

Abstract: To observe the effect of orexin A on the expression of OX 1 R in ovine luteinized granulosa cells in vitro,the cells were treated with different concentrations (0.05,0.2,0.5,1 μg/mL) of orexins A.Then the total RNA was extracted from the cells after treated in 0,2,6,12 h,and the mRNA expression levels of OX1R were eval-uated using reverse transcription quantitative PCR.In result,orexin A induced release of OX1R in ovine luteinized granulosa cells.In high concentration (1 μg/mL) group,the OX1R mRNA expression level was increased to the highest in 2 h(P<0.01) and gradually weakened since then;in low concentration(0.05,0.2,0.5μg/mL) group, the OX1R mRNA expression level was increased in 12 h and the expression level of 0.2 μg/mL group was in-creased to the highest in 12 h(P<0.01).Orexin A induced expressions of OX1R mRNA,the group of 1 μg/mL was more rapid up regulation compared with the low concentration (0.05,0.2,0.5 μg/mL) group.

Key words: ORexin A, OX1R, Sheep, Luteinized gRanulosa cells

中图分类号: 

• S826