摘要： 为了探讨PRLH及其受体基因在绵羊性腺轴中的表达,以绵羊下丘脑-垂体-性腺轴系为研究对象,克隆了PRLH及其受体基因PRLHR,构建了系统进化树,并利用qRT-PCR技术对PRLH及其受体基因在绵羊性腺轴中不同组织的表达差异做了研究。结果表明,绵羊PRLH和PRLHR mRNA核苷酸序列分别为 113,237 bp,PRLH基因核苷酸序列与山羊、牛、人、小鼠的同源性分别为96.8%,93.3%,80.3%,74.6%,PRLHR基因与藏羚羊、山羊、牛、人和小鼠的同源性分别为100%,99.3%,97.3%,90.4%,84.4%;PRLH及其受体基因在绵羊的下丘脑、垂体、子宫和卵巢中均有一定量的表达,且PRLH基因在下丘脑、垂体和卵巢中的表达要显著低于子宫(P<0.05);PRLHR在子宫与垂体中的表达差异显著(P<0.05),其他差异不明显。 说明下丘脑、垂体、子宫和卵巢是PRLH合成分泌和发挥作用的主要器官。

关键词: 绵羊, PRLH, PRLHR, RT-PCR, 差异表达

Abstract: To explore the expression of PRLH and its receptor gene in sheep gonadal axis,we successfully cloned the PRLH and receptor gene in organizations at the hypothalamic-pituitary-gonadal axis by used the sheep as experimental animal,got the phylogenetic tree.Also we used qRT-PCR technology to research different expression of PRLH and its receptor gene in different tissues at HPG axis.The results showed that sheep PRLH and PRLHR nucleotide sequences of mRNA were 113,237 bp.PRLH gene nucleotide sequence and goats,cattle,human,mouse homology were 96.8%,93.3%,80.3%,74.6%.PRLHR gene and the Tibetan antelope,goat,cattle,human and mouse homology were 100%,99.3%,97.3%,90.4%,84.4%.PRLH and its receptor gene in sheep hypothalamus,pituitary,uterine and ovary were expressed,and the expression of PRLH gene in hypothalamus,pituitary and ovary was significantly less than the uterine (P<0.05).Expression of PRLHR in uterus and pituitary were significantly different (P<0.05),no significant difference in other tissues (P>0.05).So the hypothalamus,pituitary,ovary and uterus were the main organ for the synthesis of secretion.

Key words: Sheep, PRLH, PRLHR, RT-PCR, Differential expression

中图分类号: 

• Q78