摘要： 采用测序和PCR-RFLP的方法,分析了MSTN基因启动子区在7个品种绵羊中的单核苷酸多态性,结果表明:存在2个SNP位点(-959T/C,-784G/A),表现为6种基因型(TT、TC、CC、GG、GA、AA),在-959T/C位点中,国外肉用绵羊品种陶赛特、德国肉用美利奴及我区的巴士拜羊,TT为优势基因型,地方品种绵羊巴音布鲁克羊、多浪羊和阿勒泰羊在该位点中CC为优势基因型,经χ2检验,其群体的基因频率和基因型频率均处于Hardy-Weinberg平衡状态,群体遗传多态性分析表明,这6个绵羊品种中群体的多态信息含量(PIC)均处于0.25～0.50之间,为中度多态,特克赛尔羊在该位点未发生突变。在-784G/A位点中,除陶赛特与特克赛尔羊在该位点未发生突变,其他5个品种绵羊优势等位基因均为G等位基因。经χ2检验后,其群体的基因频率和基因型频率均处于Hardy-Weinberg平衡状态,群体遗传多态性分析表明,多浪羊、巴士拜羊和巴音布鲁克羊的群体多态信息含量(PIC)均处于0.25～0.50之间,为中度多态,而阿勒泰羊和德国肉用美利奴的群体多态信息含量(PIC)小于0.25,为低度多态。
关键词:
绵羊,
MSTN,
启动子,
PCR-RFLP,
多态性
Abstract: Sequencing along with PCR-RFLP was applied to analyze the polymorphism of MSTN gene promoter?in seven sheep breeds. Two SNPs at －959(T/C)and －784(G/A) including six genotype (TT，TC，CC，GG，GA，?AA)respectively were detected in the promoter region，At －959(T/C)，the predominant genotype of TT was identified in Dorset，German Merino and Bashibai sheep，the CC in Bayinbuluke，Duolang and Aletai sheep. A chi-square?analysis suggested that the allele frequencies and genotype frequencies of two polymorphisms were in Hardy-Weinberg equilibrium. Statistical analysis also revealed the variation of intermediate polymorphism information content?(PIC) in six breeds (0. 25 ＜ PIC ＜0. 50). At －784(G/A)，The G was predominant allele in all breeds，whereas?the low polymorphism information content (PIC) was observed in German Meilin and Aletai populations (PIC ＜?0. 25) and intermediate PIC in the Bayinbuluke，Duolang and Bashibai populations (0. 25 ＜ PIC ＜0. 50).
Key words:
Sheep,
MSTN,
Promoter,
PCR-RFLP,
Polymorphsim
中图分类号:
贺三刚, 陈芯, 刘晨曦, 刘国松, 刘明军. 绵羊MSTN基因启动子区SNPs分析[J]. 华北农学报, 2012, 27(3): 81-85. doi: 10.3969/j.issn.1000-7091.2012.03.016.
HE San-gang, CHEN Xin, LIU Chen-xi, LIU Guo-song, LIU Ming-jun. Analysis of SNPs in Promoter Region of Myostatin in Sheep[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 81-85. doi: 10.3969/j.issn.1000-7091.2012.03.016.