大豆二酰甘油酰基转移酶基因 <i>GmDGAT1A</i>启动子的克隆与功能分析

doi:10.7668/hbnxb.20190821

摘要/Abstract

摘要： 二酰甘油酰基转移酶（DGAT）是催化三酰甘油生物合成的关键酶，在三酰甘油的合成和积累过程中具有重要调控作用。为了研究大豆DGAT基因表达调控的分子机制，以大豆品种科丰1号为材料，通过PCR方法对GmDGAT1A的启动子（promoter-GmDGAT1A，pGmDGATIA）进行克隆，并通过转化拟南芥和GUS组织定位研究其功能。结果表明：以大豆叶片DNA为模板，成功克隆到GmDGAT1A基因ATG上游2 192 bp启动子序列。序列分析表明，pGmDGAT1A除具有启动子所必需的TATA-box和CAAT-box等基本顺式作用元件外，还含有多个响应于光、赤霉素和脱落酸等顺式作用元件。以GUS为报告基因，成功构建了植物表达载体pCAMBIA1381Z-pGmDGAT1A，并转化野生型拟南芥获得转基因植株。对转基因拟南芥植株进行PCR检测，能扩增到2 192 bp目标条带，表明已获得含有pGmDGAT1A的转基因拟南芥阳性植株。GUS组织化学染色结果显示，转基因拟南芥幼苗的叶脉和根染色较深，但是主根和侧根的根尖部分未染色；成熟期转基因拟南芥植株的根、叶脉以及角果内的隔膜和珠柄染色较深，茎和发育的种子未染色，表明pGmDGAT1A驱动的GUS主要在转基因拟南芥的根、叶脉以及角果内的隔膜和珠柄中表达。综上，克隆的大豆GmDGAT1A启动子具有活性，能够驱动下游目标基因的表达，有望应用于转基因育种。

关键词: 大豆, 二酰甘油酰基转移酶(DGAT), 启动子, 油脂, GUS

Abstract: Diacylglycerol acyltransferase (DGAT) is a key enzyme in the biosynthesis of triacylglycerol,which plays an important role in the synthesis and accumulation of triacylglycerol. In order to study the molecular mechanism of DGAT gene expression regulation in soybean,the promoter of GmDGAT1A (promoter-GmDGAT1A,pGmDGATIA) was cloned from the variety Kefeng 1 by using PCR amplification,and its function was analyzed by transforming Arabidopsis thaliana and GUS tissue localization. The results showed that:With leaf DNA as template,the 2 192 bp promoter fragment of GmDGAT1A was successfully cloned from the variety Kefeng 1. Sequence analysis showed that p GmDGAT1A not only had the basic cis-acting elements such as TATA-box and CAAT-box,but also contained a number of cis-acting elements responsive to light,gibberellin and abscisic acid. The plant expression vector pCAMBIA1381Z-p GmDGAT1A was successfully constructed using GUS as a reporter gene and transformed into wild type Arabidopsis thaliana to obtain transgenic plants. The 2 192 bp band could be amplified from the transgenic Arabidopsis thaliana plants by PCR,indicating that a transgenic line containing p GmDGAT1A was obtained. GUS histochemical staining of transgenic Arabidopsis thaliana seedlings showed that the veins and roots were deeply stained,but the root tips of main roots and lateral roots were not stained. For mature Arabidopsis thaliana plants,enhanced GUS staining was observed in roots,veins and diaphragms and funiculi of siliques,while the stems and seeds were not stained. These suggested that p GmDGAT1A -driven GUS was mainly expressed in roots,veins and diaphragms and funiculi of siliques. In conclusion,the cloned promoter of GmDGAT1A has the activity,can drive the expression of downstream target gene,and may be used in transgenic breeding.

Key words: Soybean, Diacylglycerol acyltransferase (DGAT), Promoter, Oil, GUS

中图分类号:

S565.03
Q78

晁毛妮, 胡喜贵, 张晋玉, 王润豪, 温青玉, 孙新凯, 黄中文. 大豆二酰甘油酰基转移酶基因 GmDGAT1A启动子的克隆与功能分析[J]. 华北农学报, 2020, 35(4): 27-34. doi: 10.7668/hbnxb.20190821.

CHAO Maoni, HU Xigui, ZHANG Jinyu, WANG Runhao, WEN Qingyu, SUN Xinkai, HUANG Zhongwen. Cloning and Functional Analysis of Promoter of Diacylglycerol Acyltransferase Gene GmDGAT1A in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 27-34. doi: 10.7668/hbnxb.20190821.

http://www.hbnxb.net/CN/Y2020/V35/I4/27

参考文献

[1] 李晓丹, 吴刚, 武玉花,肖玲,卢长明. 大豆种子发育过程中脂肪酸积累模式研究[J]. 大豆科学, 2007,26(4):506-510. doi:10.3969/j.issn.1000-9841.2007.04.011. Li X D,Wu G,Wu Y H, Xiao L,Lu C M. The accumlation pattern of fatty acids dueing the development of soybean seeds[J]. Soybean Science,2007,26(4):506-510.
[2] 邹筱. 大豆脂肪和脂肪酸主要组分含量QTL定位[D]. 北京:中国农业科学院, 2014. Zou X. Quantitative trait loci of oil and main fatty acid components in soybean[D]. Beijing:Chinese Academy of Agricultural Sciences,2014.
[3] 郑永战,盖钧镒,卢为国,李卫东, 周瑞宝, 田少君. 大豆脂肪及脂肪酸组分含量的QTL定位[J]. 作物学报,2006,32(12):1823-1830. doi:10.3321/j.issn:0496-3490.2006.12.008. Zheng Y Z,Gai J Y,Lu W G,Li W D,Zhou R B,Tian S J. QTL mapping for fat and fatty acid composition contents in soybean[J]. Acta Agronomica Sinica,2006,32(12):1823-1830.
[4] Baud S,Lepiniec L. Physiological and developmental regulation of seed oil production[J]. Progress in Lipid Research,2010,49(3):235-249. doi:10.1016/j.plipres.2010.01.001.
[5] Lassner M W,Lardizabal K,Metz J G. A jojoba beta-Ketoacyl-CoA synthase cDNA complements the canola fatty acid elongation mutation in transgenic plants[J]. The Plant Cell,1996,8(2):281-292. doi:10.1105/tpc.8.2.281.
[6] Chapman K D,Ohlrogge J B. Compartmentation of triacylglycerol accumulation in plants[J]. Journal of Biological Chemistry,2012,287(4):2288-2294. doi:10.1074/jbc.R111.290072.
[7] Bates P D,Stymne S,Ohlrogge J. Biochemical pathways in seed oil synthesis[J]. Current Opinion in Plant Biology,2013,16(3):358-364. doi:10.1016/j.pbi.2013.02.015.
[8] Napier J A,Haslam R P,Beaudoin F, Cahoon E B. Understanding and manipulating plant lipid composition:Metabolic engineering leads the way[J]. Current Opinion in Plant Biology,2014,19(100):68-75. doi:10.1016/j.pbi.2014.04.001.
[9] Lung S C,Weselake R J. Diacylglycerol acyltransferase:A key mediator of plant triacylglycerol synthesis[J]. Lipids,2006,41(12):1073-1088. doi:10.1007/s11745-006-5057-y.
[10] 陶芬芳,邢蔓,岳宁燕, 邬贤梦. 植物三酰甘油合成相关基因研究进展[J]. 作物研究,2017,31(3):330-336. doi:10.16848/j.cnki.issn.1001-5280.2017.03.27. Tao F F,Xing M,Yue N Y,Wu X M. Research advances of genes related to plant triacylglycerol synthesis[J]. Crop Research,2017,31(3):330-336.
[11] Xu J Y,Francis T,Mietkiewska E,Giblin E M,Barton D L,Zhang Y,Zhang M,Taylor D C. Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1(DGAT1) gene from Tropaeolum majus,and a study of the functional motifs of the DGAT protein using site-directed mutagenesis to modify enzyme activity and oil content[J]. Plant Biotechnology Journal, 2008,6(8):799-818. doi:10.1111/j.1467-7652.2008.00358.x.
[12] 李欢, 赵翠珠, 李长圣, 刘明喆, 张文学, 李志西, 张猛. 甘蓝型油菜 DGAT1 重复基因的克隆与表达分析[J]. 西北农业学报, 2014,23(7):55-61. doi:10.7606/j.issn.1004-1389.2014.07.010. Li H,Zhao C Z,Li C S,Liu M Z,Zhang W X,Li Z X,Zhang M. Molecular cloning and expression annlysis of Brassica napus DGAT1 duplicate genes[J]. Acta Agriculturae Boreali-Occidentalis Sinica,2014,23(7):55-61.
[13] Chen B B, Wang J J, Zhang G Y, Liu J Q, Manan S, Hu H H, Zhao J. Two types of soybean diacylglycerol acyltransferases are differentially involved in triacylglycerol biosynthesis and response to environmental stresses and hormones[J]. Scientific Reports,2016,6:28541. doi:10.1038/srep28541.
[14] Zheng P Z, Allen W B, Roesler K, Williams M E, Zhang S R, Li J M, Glassman K, Ranch J, Nubel D, Solawetz W, Bhattramakki D, Llaca V, Deschamps S, Zhong G Y, Tarczynski M C, Shen B. A phenylalanine in DGAT is a key determinant of oil content and composition in maize[J]. Nature Genetics,2008,40(3):367-372. doi:10.1038/ng.85.
[15] Bouvier-Navé P,Benveniste P,Oelkers P, Sturley S L,Schaller H. Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:Diacylglycerol acyltransferase[J]. FEBS Journal,2010,267(1):85-96. doi:10.1046/j.1432-1327.2000.00961.x.
[16] Sanjaya,Miller R,Durrett T P,Kosma D K,Lydic T A,Muthan B,Koo A J K,Bukhman Y V,Reid G E,Howe G A,Ohlrogge J,Benning C. Altered lipid composition and enhanced nutritional value of Arabidopsis leaves following introduction of an algal diacylglycerol acyltransferase 2[J]. The Plant Cell,2013,25(2):677-693. doi:10.1105/tpc.112.104752.
[17] Liu Q,Siloto R M P,Lehner R,Stone S J,Weselake R J. Acyl-CoA:Diacylglycerol acyltransferase:molecular biology,biochemistry and biotechnology[J]. Progress in Lipid Research,2012,51(4):350-377. doi:10.1016/j.plipres.2012.06.001.
[18] Shockey J M,Gidda S K,Chapital D C,Kuan J C,Dhanoa P K,Bland J M,Rothstein S J,Mullen R T,Dyer J M. Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum[J]. The Plant Cell,2006,18(9):2294-2313. doi:10.1105/tpc.106.043695.
[19] Banilas G,Karampelias M,Makariti I,Kourti A,Hatzopoulos P. The olive DGAT2 gene is developmentally regulated and shares overlapping but distinct expression patterns with DGAT1[J]. Journal of Experimental Botany,2011,62(2):521-532. doi:10.1093/jxb/erq286.
[20] Zou J T,Wei Y D,Jako C,Kumar A,Selvaraj G,Taylor D C. The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene[J]. The Plant Journal,1999,19(6):645-653. doi:10.1046/j.1365-313x.1999.00555.x.
[21] Jako C,Kumar A,Wei Y D,Zou J T,Barton D L,Giblin E M,Covello P S,Taylor D C. Seed-specific over-expression of an Arabidopsis cDNA encoding adiacylglycerol acyltransferase enhances seed oil content and seed weight[J]. Plant Physiology,2001,126(2):861-874. doi:10.1104/pp.126.2.861.
[22] Zhang M,Fan J L,Taylor D C,Ohlrogge J B. DGAT1 and PDAT1 acyltransferases have overlapping functions in Arabidopsis triacylglycerol biosynthesis and are essential for normal pollen and seed development[J]. The Plant Cell,2009,21(12):3885-3901. doi:10.1105/tpc.109.071795.
[23] 刘贵芹,邵群,黄荣峰,冯献忠. 大豆DGAT基因家族的鉴定和表达分析[J]. 中国农学通报,2013,29(12):55-61. doi:10.3969/j.issn.1000-6850.2013.12.011. Liu G Q,Shao Q,Huang R F,Feng X Z. Characterization and expression analysis of DGAT gene family in soybean[J]. Chinese Agricultural Science Bulletin,2013,29(12):55-61.
[24] 赵江哲. 大豆磷脂酶基因 GmPLD 和脂合成酶基因 GmDGAT, GmLPAT 在调控拟南芥生长和种子油含量中的作用[D].南京:南京农业大学,2013. doi:10.7666/d.Y2528378. Zhao J Z. Phospholipase GmPLD and lipid synthetase GmDGAT from soybean regulate growth and seed oil in transgenic Arabidopsis plants[D]. Nanjing:Nanjing Agricultural University,2013.
[25] Connors B J,Miller M,Maynard C A,Powell W A. Cloning and characterization of promoters from American chestnut capable of directing reporter gene expression in transgenic Arabidopsis plants[J]. Plant Science,2002,163(4):771-781. doi:10.1016/S0168-9452(02)00214-5.
[26] 文添龙,刘雪梅,冀亚萍,俞嘉宁. 高等植物胁迫诱导型启动子的研究进展[J]. 西北植物学报,2014,34(1):206-214. doi:10.7606/j.issn.1000-4025.2014.01.0206. Wen T L,Liu X M,Ji Y P,Yu J N. Research progress of stress-induced promoter in higher plant[J]. Acta Botanica Boreali-Occidentalia Sinica,2014,34(1):206-214.
[27] 吕兆勇,赵春梅,薛仁镐. 葡萄逆境胁迫诱导启动子的克隆及表达分析[J].华北农学报, 2016, 31(1):77-82. doi:10.7668/hbnxb.2016.01.013. Lü Z Y,Zhao C M,Xue R G. Cloning and expression analysis of grape's stress inducible promoter[J]. Acta Agriculturae Boreali-Sinica,2016, 31(1):77-82.
[28] 翟莹,张军,任巍巍,张闯,赵艳,张梅娟. 大豆低温诱导启动子 GmERF9P 的克隆及活性鉴定[J]. 华北农学报, 2017,32(5):13-18. doi:10.7668/hbnxb.2017.05.003. Zhai Y,Zhang J,Ren W W,Zhang C,Zhao Y,Zhang M J.Cloning and activity analysis of cold induced GmERF9P promoter from soybean[J]. Acta Agriculturae Boreali-Sinica, 2017,32(5):13-18.
[29] Li R Z,Hatanaka T,Yu K S,Wu Y M,Fukushige H,Hildebrand D. Soybean oil biosynthesis:role of diacylglycerol acyltransferases[J]. Functional Integrative Genomics,2013,13(1):99-113. doi:10.1007/s10142-012-0306-z.
[30] Clough S J,Bent A F. Floral dip:a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana[J]. The Plant Journal,1998,16(6):735-743. doi:10.1046/j.1365-313x.1998.00343.x.
[31] Jefferson R A,Kavanagh T A,Bevan M W. GUS fusions:Beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants[J]. EMBO Journal,1987,6(13):3901-3907. doi:10.1002/j.1460-2075.1987.tb02730.x.
[32] 张江江,常丽,严江涛,赵立宁,李德芳.作物高油品种选育策略研究进展[J].河南农业科学,2019,48(6):1-7,13. doi:10.15933/j.cnki.1004-3268.2019.06.001. Zhang J J,Chang L,Yan J T,Zhao L N,Li D F. Research progress of breeding strategy of high oil crop varieties[J]. Journal of Henan Agricultural Sciences,2019,48(6):1-7,13.
[33] 陈玉梅,李璐璐,陈锦玲,徐媛,李惠敏,秦新民.基于转录组测序的花生籽粒不同发育时期油脂合成相关基因差异表达分析[J].河南农业科学,2019,48(7):24-37. doi:10.15933/j.cnki.1004-3268.2019.07.005. Chen Y M,Li L L,Chen J L,Xu Y,Li H M,Qin X M. Differential expression analysis of genes related to lipid synthesis through transcriptome sequencing during different developmental stages in peanut seed[J]. Journal of Henan Agricultural Sciences,2019,48(7):24-37.
[34] 王颖, 麦维军, 梁承邺,张明永. 高等植物启动子的研究进展[J]. 西北植物学报,2003,23(11):2040-2046. doi:10.3321/j.issn:1000-4025.2003.11.037. Wang Y,Mai W J,Liang C Y,Zhang M Y. Advances on studies of plant promoters[J] . Acta Botanica Boreali-Occidentalia Sinica,2003,23(11):2040-2046.
[35] 伍静辉,谢楚萍,田长恩,周玉萍. 脱落酸调控种子休眠和萌发的分子机制[J]. 植物学报,2018,53(4):542-555. doi:10.11983/CBB18080. Wu J H,Xie C P,Tian C E,Zhou Y P. Molecular mechanism of abscisic acid regulation during seed dormancy and germination[J]. Chinese Bulletin of Botany,2018,53(4):542-555.
[36] Nambara E,Naito S,McCourt P. A mutant of Arabidopsis which is defective in seed development and storage protein accumulation is a new abi3 allele[J]. The Plant Journal,1992,2(4):435-441. doi:10.1111/j.1365-313X.1992.00435.x.
[37] Finkelstein R R,Somerville C R. Three classes of abscisic acid (ABA)-insensitive mutations of Arabidopsis define genes that control overlapping subsets of ABA responses[J]. Plant Physiology,1990,94(3):1172-1179. doi:10.1104/pp.94.3.1172.
[38] 崔力勃, 朱乐, 蒋立希. ABA对于十字花科油籽脂肪酸组成及储藏蛋白的影响及机制[J]. 农业生物技术学报,2017,25(7):1059-1071. doi:10.3969/j.issn.1674-7968.2017.07.003. Cui L B,Zhu L,Jiang L X. The effect of ABA on biosynthesis of fatty acids and storage proteins and the relevant mechanism in Cruciferae oilseed[J]. Journal of Agricultural Biotechnology,2017,25(7):1059-1071.
[39] Singh D P,Filardo F F,Storey R,Jermakow A M,Yamaguchi S,Swain S M. Overexpression of a gibberellin inactivation gene alters seed development, KNOX gene expression,and plant development in Arabidopsis[J]. Physiologia Plantarum,2010,138(1):74-90. doi:10.1111/j.1399-3054.2009.01289.x.
[40] Chen M X, Du X, Zhu Y, Wang Z, Hua S J, Li Z L, Guo W L, Zhang G P, Peng J R, Jiang L X. Seed fatty acid reducer acts downstream of gibberellin signalling pathway to lower seed fatty acid storage in Arabidopsis[J]. Plant Cell Environment,2012,35(12):2155-2169. doi:10.1111/j.1365-3040.2012.02546.x.
[41] 李志兰,华水金,蒋立希. 植物GDSL脂酶家族基因的研究进展[J]. 农业生物技术学报,2014,22(7):916-924. doi:10.3969/j.issn.1674-7968.2014.07.016. Li Z L,Hua S J,Jiang L X. Advances in studying plant GDSL-type lipase genes[J]. Journal of Agricultural Biotechnology,2014,22(7):916-924.
[42] Rushton P J,Reinstädler A,Lipka V,Lippok B,Somssich I E. Synthetic plant promoters containing defined regulatory elements provide novel insights into pathogen-and wound-induced signaling[J]. The Plant Cell,2002,14(4):749-762. doi:10.1105/tpc.010412.
[43] Kroon J T M,Wei W X,Simon W J,Slabas A R. Identification and functional expression of a type 2 acyl-CoA:Diacylglycerol acyltransferase (DGAT2) in developing castor bean seeds which has high homology to the major triglyceride biosynthetic enzyme of fungi and animals[J]. Phytochemistry,2006,67(23):2541-2549. doi:10.1016/j.phytochem.2006.09.020.
[44] 曹仪植. 拟南芥[M]. 北京:高等教育出版社, 2004. Cao Y Z. A. thaliana[M]. Beijing:Higher Education Press,2004.

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大豆二酰甘油酰基转移酶基因 GmDGAT1A启动子的克隆与功能分析

Cloning and Functional Analysis of Promoter of Diacylglycerol Acyltransferase Gene GmDGAT1A in Soybean

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大豆二酰甘油酰基转移酶基因 GmDGAT1A启动子的克隆与功能分析

Cloning and Functional Analysis of Promoter of Diacylglycerol Acyltransferase Gene GmDGAT1A in Soybean

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