谷子酸性磷酸酶ACP家族基因鉴定与 <i>SiACP1</i>耐低磷单倍型分析

doi:10.7668/hbnxb.20191202

摘要/Abstract

摘要： 细胞内酸性磷酸酶（ACP）是一种将液泡内的磷酸酯水解为无机磷的酶，普遍存在于植物组织中，在调控植物磷营养方面起着重要作用。谷子具有耐贫瘠特性，蕴藏着重要的耐低磷优异位点。挖掘谷子SiACP基因的关键变异位点和单倍型，促进分子标记辅助培育新品种，是解决土壤有效磷缺乏的主要遗传育种途径之一。通过同源序列比对的方式共获得了13个谷子以及其他3种禾本科作物的ACP家族基因，并对其进行生物信息学分析，结果发现，ACP家族基因在禾本科作物的数量和分布具有一定的规律性，均具有高度保守的磷酸酶结构域和基序。通过家族进化树分析发现，单子叶植物ACP基因与双子叶植物ACP基因在进化过程中出现明显的分支。转录组数据的表达模式结果显示，C4植物谷子的SiACP1基因表达模式具有明显的组织表达特异性，而SiACP2和SiACP3基因在整个生长发育阶段的不同组织表达量均较高。通过候选基因关联分析和单倍型分析发现，位于SiACP1启动子的SNP与耐低磷相关性状显著关联，并初步鉴定到一个与谷子耐低磷性状相关的有利单倍型ACPHap2。研究结果将为作物耐低磷优良种质育种提供一定的基因遗传信息参考。

关键词: 谷子, ACP家族基因, 耐低磷, 单倍型

Abstract: Intracellular acid phosphatase (ACP) is an enzyme which hydrolyzes phospholipids in vacuoles to inorganic phosphorus. The ACP enzyme is also ubiquitous in plant tissues and plays an important role in regulating plant phosphorus nutrition. It will be one of the main genetic and breeding approaches to solve the deficiency of available phosphorus in soil by developing new varieties assisted superior alleles and haplotypes in SiACP genes. First identified 13 ACP family genes form foxtail millet and other three gramineous crops by homologous alignment. After systematic bioinformatics analysis,it was found that the number and distribution of ACP family genes in gramineous crops had certain regularity,and they all had highly conserved phosphatase domains and motifs. In addition,through the analysis of family evolutionary tree,it was found that the ACP genes of monocotyledons and dicotyledons had obvious branches in the evolutionary process. Finally,expression pattern analysis of transcriptome data showed that SiACP1 genes in foxtail millet apparently exhibited a tissue specific expression,while the higher expression of SiACP2 and SiACP3 genes was found in different tissues through the growth and development stage. Meanwhile,through candidate gene association analysis and haplotype analysis,SNPs located in SiACP1 promoter was significantly associated with the traits with low phosphorus tolerance. Interestingly,a haplotype ACPHap2 which was favorable to low phosphorus tolerance of foxtail millet was preliminarily identified. Research results will provide some genetic information reference for the discovery of excellent germplasm with low phosphorus tolerance and molecular breeding of crops in further.

Key words: Foxtail millet, ACP family gene, Low phosphorus tolerance, Haplotype

中图分类号:

S515.03
Q78

赵雄伟, 吴年隆, 乔佳辉, 李旭凯, 韩渊怀, 邢国芳. 谷子酸性磷酸酶ACP家族基因鉴定与 SiACP1耐低磷单倍型分析[J]. 华北农学报, 2020, 35(4): 35-45. doi: 10.7668/hbnxb.20191202.

ZHAO Xiongwei, WU Nianlong, QIAO Jiahui, LI Xukai, HAN Yuanhuai, XING Guofang. Identification of the Acid Phosphatase-encoding Family Genes in Foxtail Millet and SiACP1 Haplotype Analysis with Low Phosphorus Tolerance[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 35-45. doi: 10.7668/hbnxb.20191202.

http://www.hbnxb.net/CN/Y2020/V35/I4/35

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谷子酸性磷酸酶ACP家族基因鉴定与 SiACP1耐低磷单倍型分析

Identification of the Acid Phosphatase-encoding Family Genes in Foxtail Millet and SiACP1 Haplotype Analysis with Low Phosphorus Tolerance

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谷子酸性磷酸酶ACP家族基因鉴定与 SiACP1耐低磷单倍型分析

Identification of the Acid Phosphatase-encoding Family Genes in Foxtail Millet and SiACP1 Haplotype Analysis with Low Phosphorus Tolerance

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