摘要： 为从分子水平研究甘肃境内典型养殖模式下甘肃高山细毛羊、小尾寒羊、藏羊、蒙古羊和滩羊5个绵羊品种的PRKAG3基因多态性与屠宰性能和肉品质的相关性,探讨以PRKAG3为候选基因,提高绵羊屠宰性能和肉品质的可能性。采用PCR-SSCP技术对5个绵羊品种的PRKAG3基因外显子4,5和内含子4多态性进行检测,并就PRKAG3基因多态性与9月龄屠宰性能和肉品质进行相关性分析。结果表明,在PRKAG3基因2689 bp处发生了A>G的突变,位于第4内含子第23 bp处,表现出3种基因型,AA、AB和BB。纯合度(Ho)分别为0.67,0.69,0.76,0.69,0.59,杂合度(He)分别为0.3333,0.3061,0.2449,0.3077,0.4082,有效等位基因(Ne)分别为1.62,1.66,1.69,1.48,1.79,多态信息含量(PIC)分别为0.31,0.32,0.33,0.27,0.34。除藏羊外其他4个绵羊品种均处于Hardy-Weinberg平衡状态。关联分析表明,5个绵羊品种PRKAG3基因此变异位点的3个基因型间失水率性状差异显著(P<0.05);BB基因型个体的胴体重显著高于AA型(P<0.05),滩羊为极显著(P<0.01),BB基因型个体的眼肌面积显著大于AA型(P<0.05)(藏羊除外)。因此说明PRKAG3基因可作为候选基因用于绵羊产肉性能和肉品质的分子标记辅助选择。

关键词: 绵羊, PCR-SSCP, PRKAG3 基因, 肉质性状, 关联性分析

Abstract: In order to study the relationship between the PRKAG3 gene polymorphism and slaughter performance,meat quality of 5 sheep breeds(Gansu alpine fine wool sheep,Small tail han sheep,Tibetan,Mongolian sheep and Tan-sheep which were fed in a typical farming mode of Gansu province) in Gansu province at the molecular level and to explore the possibility of improving the slaughter performance and quality of sheep meat by using PRKAG3 as candidate gene,the polymorphism of exon 4,5and introns 4,a total of 321 bp,of PRKAG3 gene was detected by PCR-SSCP in 5 sheep breeds,and the association between PRKAG3 gene polymorphism and slaughter performance,meat quality in aged 9 month were analyzed.The results showed:that B allele had a base transformation of A>G at the 2 689th bp in intron 4 formed AA,AB and BB genetypes.The gene homozygosity (Ho)were 0.67,0.69,0.76,0.69 and 0.59 respectively;the heterozygosity were 0.333 3,0.306 1,0.244 9,0.307 7 and 0.408 2,respectively;the effective number of alleles (Ne) were 1.62,1.66,1.69,1.48 and 1.79;the polymorphism information content (PIC) were 0.31,0.32,0.33,0.27 and 0.34,respectively.In addition to the Tibetan sheep other four sheep breeds on this loci in a Hardy-Weinberg equilibrium state by Chi-square test.Association analysis of the SNPs with meat quality traits was carried out.The result showed that the mutation loc of PRKAG3 gene was significantly associated (P<0.05) with meat water loss rate,genotype BB between individual's body weight was significantly higher than AA type (P<0.05),Tan-sheep was very significant(P<0.01).The eye muscle area was significantly greater between a BB genotype and AA type (P<0.05).So, PRKAG3 gene can be used as a candidate gene for marker-assisted selection of sheep meat performance and meat quality.

Key words: Sheep, PCR-SSCP, PRKAG3 gene, Meat quality traits, Association analysis

中图分类号: 

• Q78

• S858.03