摘要： 为阐明 GnRHR 基因的多态性与崂山奶山羊产羔数的关系,设计7对引物,采用PCR-RFLP和PCR-SSCP技术检测 GnRHR 基因在不同产羔数崂山奶山羊的单核苷酸多态性,并分析其与产羔数的关系。结果显示:该基因存在2个多态位点,A261G突变对于初产母羊GA型产羔数比AA型多0.06只,比GG型多0.04只,差异不显著(P>0.05);对于经产母羊GA型产羔数比AA型多0.35只,差异显著(P<0.05),比GG型多0.45只,差异不显著(P>0.05)。G55A位点共检测到GG和GA这2种基因型,其中对于初产母羊GG型比GA型产羔数多0.13只,经产母羊GG型比GA型产羔数多0.19只,但差异均不显著(P>0.05)。因此, GnRHR 基因A261G突变位点可能是影响崂山奶山羊产羔性能一个潜在的有效分子标记。

关键词: GnRHR 基因, 奶山羊, 产羔数

Abstract: The objectives of the present study were to elucidate the polymorphism of GnRHR gene and its relationship with litter size of Laoshan dairy goat.Seven pairs of primers were designed to detect single nucleotide polymorphism (SNP) of GnRHR gene in Laoshan dairy goat with different kids by PCR-RFLP and PCR-SSCP.The results showed two mutations (A261G and G55A) were detected.For A261G locus in exon1,the does with genotype GA had 0.06 and 0.04 kids more than those with genotype AA and GG in primipara ewes,but the differences were not significant (P>0.05);the does with genotype GA had 0.35 and 0.45 kids more than those with genotype AA and GG in multiparity ewes (P>0.05).For G55A locus,two genotypes (GG and GA) were detected,the does with genotype GG had 0.13 and 0.19 more than those with genotype GA in primipara ewes and multiparity ewes,respectively.However the differences were not significant (P>0.05).These results preliminarily indicated that A261G locus of GnRHR gene is a potential molecular marker for improving litter size in dairy goats.

Key words: GnRHR gene, Dairy goats, Litter size

中图分类号: 

• S826.03