河北省130份西瓜品种与种质资源抗病基因KASP检测分析

doi:10.7668/hbnxb.201751582

摘要/Abstract

摘要： 旨在筛选河北地区抗枯萎病、炭疽病、白粉病西瓜品种与资源，分析其所含有的抗病基因，为筛选和培育抗病西瓜新品种提供参考。采用改良后的CTAB法提取西瓜种芽DNA，并用超微量分光光度计检测DNA浓度，将其稀释至2~10 ng/μL后使用许勇团队开发的西瓜抗枯萎病、抗炭疽病、抗白粉病分子标记，利用高通量分型KASP标记技术对西瓜抗枯萎病、抗炭疽病和抗白粉病性状相关的基因进行了检测。试验材料为河北省近年来审（鉴）定西瓜品种和优势组合（星研七号、美佳、美胜、贵妃、17-11等）以及优异种质资源（花早绿、JB-1、JB-3、901新等）共130份材料，抗病对照为3抗302，感病对照为GBZG。结果表明，所有被检测材料中33份材料含抗枯萎病基因（Fon-1），19份材料含抗炭疽病基因（AR1），7份材料含抗白粉病基因（PM1），5份材料同时含Fon-1和AR1，1份材料同时含AR1和PM1，1份材料同时含Fon-1、AR1、PM1 3种抗病基因。根据KASP检测结果对试验材料进行聚类分析，将试验材料分为4类，分别为抗白粉病基因处均无检测信号或杂合抗性12份、抗白粉病或同时感2种病害材料67份、抗枯萎病或抗炭疽病材料38份，抗枯萎病、抗炭疽病或抗白粉病基因方面均无检测信号或杂合抗性13份。

关键词: 西瓜, 抗病基因, KASP检测

Abstract: This study aimed to screen the watermelon varieties and germplasms resistant to fusarium wilt, anthracnose and powdery mildew in Hebei Province, and analyze the related resistance genes, which could provide a reference for breeding disease-resistant varieties. The modified CTAB method was used to extract the DNA from watermelon bud, and the DNA concentration was determined by Ultramicro spectrophotometer. After the DNA was diluted to 2-10 ng/μL, they were detected by KASP. The molecular markers of resistance to fusarium wilt, anthracnose and powdery mildew of watermelon were developed by XU Yong's team. The resistant genes related to fusarium wilt, anthracnose and powdery mildew of watermelon were identified by high-throughput KASP markers. Totally 130 watermelon materials were tested, including varieties or advantaging combinations (Xingyan No.7, Meijia, Meisheng, Guifei, 17-11 and so on) that have been approved or will be approved in Hebei Province in recent years, and some excellent germplasms (Huazaolü,JB-1,JB-3,901xin and so on). 3Kang302 was used as the disease-resistant control and GBZG as the susceptible control. The result showed that 33 materials contained fusarium wilt resistance gene Fon-1, 19 contained anthracnose resistance gene AR1, 7 contained powdery mildew resistance gene PM1, 5 contained Fon-1 and AR1, 1 contained AR1 and PM1, and 1 contained all three resistance genes(Fon-1, AR1 and PM1). According to the result of KASP, the materials were clustered, and divided into 4 categories:12 materials resistant to powdery mildew without detection signal or with heterozygous resistance, 67 resistant to powdery mildew or susceptible to at least two diseases, 38 resistant to fusarium wilt or anthracnose, and 13 resistant to fusarium wilt or anthracnose or powdery mildew without detection signal or with heterozygous resistance.

Key words: Watermelon, Resistance gene, KASP detection

中图分类号:

S642.9

张敬敬, 张海英, 潘秀清, 许勇, 任毅, 高秀瑞, 李冰, 史宇凡, 党继革, 杨明珠, 武彦荣. 河北省130份西瓜品种与种质资源抗病基因KASP检测分析[J]. 华北农学报, 2019, 34(2): 110-116. doi: 10.7668/hbnxb.201751582.

ZHANG Jingjing, ZHANG Haiying, PAN Xiuqing, XU Yong, REN Yi, GAO Xiurui, LI Bing, SHI Yufan, DANG Jige, YANG Mingzhu, WU Yanrong. KASP Assays of Disease Resistance Genes from 130 Watermelon Varieties and Germplasms in Hebei Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 110-116. doi: 10.7668/hbnxb.201751582.

http://www.hbnxb.net/CN/Y2019/V34/I2/110

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