青海春小麦品种高原363成株期抗条锈病基因遗传模型分析

doi:10.7668/hbnxb.201751235

摘要/Abstract

摘要： 为明确青海春小麦品种高原363成株期抗条锈性遗传基础，分别以成株期抗条锈性青海春小麦品种高原363与感病春小麦品种Taichung 29（T29）杂交并自交创建F_2：3分离群体，通过在青海省西宁市和海东市互助县2个试验地点各2 a的田间病圃抗病性鉴定，使用单个分离世代分析法用植物数量性状主基因+多基因混合遗传模型分析高原363/T29 F₂群体的抗性遗传效应。F_2：3群体的反应型和严重度的频率分布结果表明，高原363/T29 F_2：3群体的病害严重度和反应型在西宁和互助共2个环境2 a测试下呈现双峰分布，没有出现连续性分布现象，但是又出现了不同区段内连续性变化，初步分析认为，高原363对小麦条锈病的成株期抗性是一种复杂遗传，这种遗传不仅仅由主效基因控制，同时还受到微效多基因控制，遗传模型分析结果表明，在2个不同地点测试下的高原363，无论是采用严重度数据得出的最优遗传模型还是使用反应型数据得出的最优遗传模型，都是被微效基因影响的2对主基因遗传，并且主基因的作用方式（C-1：2MG-ADI加性-显性-上位性，C-5：2MG-AED完全显性，C-6：2MG-EEAD等显性）是不同的。

关键词: 春小麦, 高原363, 抗条锈性, 遗传模型

Abstract: In order to study the genetic rule of resistance to stripe rust in Qinghai spring wheat cultivar Gaoyuan 363, Qinghai spring wheat adult stage stripe rust resistance cultivar Gaoyuan 363 was crossed with the susceptible spring wheat cultivar Taichung 29 (T29) to produce the F_2:3 segregating populations. The stripe rust resistance was identified separately in Xining and Huzhu for two years. The inheritance effects of stripe rust resistance in Gaoyuan 363/T29 F₂ populations was analyzed by the method of single generation of joint segregation analysis using mixed inheritance model of major gene plus polygene. Frequency distributions results of Gaoyuan 363/T29 F_2:3 populations the infection type and disease severity data showed that:tested on two places, Xining and Huzhu, and in two years for Gaoyuan 363/T29 F_2:3 populations, the disease severity and infection type presented a bimodal distribution, which was uncontinuous, but was continuous in some parts. It preliminarily indicated that stripe rust resistance of Gaoyuan 363 was jointly controlled by major gene and minor genes; the result of inheritance model analysis showed that, for Gaoyuan 363 tested in both environments, whether using the disease severity or the infection type data, the most fitted genetic model for stripe rust resistance was controlled by two major genes and partially by minor genes, and the two major gene interactions (C-1:2MG-ADI additive-major-epistatic interaction,C-5:2MG-AED complete dominance effect,C-6:2MG-EEAD equal dominance effect) were different.

Key words: Spring wheat, Gaoyuan 363, Stripe rust resistance, Inheritance model

中图分类号:

S435.121

侯璐. 青海春小麦品种高原363成株期抗条锈病基因遗传模型分析[J]. 华北农学报, 2019, 34(2): 117-123. doi: 10.7668/hbnxb.201751235.

HOU Lu. Inheritance Model Analysis of Adult Plant Resistance Gene in Qinghai Spring Wheat Cultivar Gaoyuan 363 to Stripe Rust[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 117-123. doi: 10.7668/hbnxb.201751235.

http://www.hbnxb.net/CN/Y2019/V34/I2/117

参考文献

[1] Chen X M.Epidemiology and control of stripe rust (Puccinia striiformis f. sp. tritici) on wheat[J]. Canadian Journal of Plant Pathology, 2005, 27:314-337.doi:10.1080/07060660509507230.
[2] 陈万权, 康振生, 马占鸿, 徐世昌, 金社林, 姜玉英. 中国小麦条锈病综合治理理论与实践[J]. 中国农业科学, 2013, 46(20):4254-4262.doi:10.3864/j.issn.0578-1752.2013.20.008.
Chen W Q, Kang Z S, Ma Z H, Xu S C, Jin S L, Jiang Y Y. Integrated management of wheat stripe rust caused by Puccinia striiformis f.[J]. Scientia Agricultura Sinica, 2013, 46(20):4254-4262.
[3] Line Roland F, Chen X M. Successes in breeding for and managing durable resistance to wheat rusts[J]. Plant Disease, 1995, 79(12):1254-1255.
[4] 姚强, 郭青云, 闫佳会, 张贵, 侯生英, 陈万权. 青海东部麦区小麦条锈菌越冬调查初报[J]. 植物保护学报, 2014, 41(5):578-583.doi:10.13802/j.cnki.zwbhxb.2014.05.030.
Yao Q, Guo Q Y, Yan J H, Zhang G, Hou S Y, Chen W Q. Survey on overwintering Puccinia striiformis f.sp. tritici at different altitudes in eastern Qinghai[J]. Acta Phytophylacica Sinica, 2014,41(5):578-583.
[5] 王建超, 冯晶, 王凤涛, 苍晶, 蔺瑞明, 冯晓琴, 徐世昌. 我国小麦农家品种小红芒成株抗条锈性遗传分析[J]. 植物保护, 2015, 41(1):154-157.doi:10.3969/j.issn.0529-1542.2015.01.031.
Wang J C, Feng J, Wang F T, Cang J, Lin R M, Feng X Q, Xu S C. Genetic analysis on the adult plant resistance of a wheat landrace Xiaohongmang to Puccinian striiformis[J]. Plant Protection, 2015,41(1):154-157.
[6] 刘太国, 邱军, 周益林, 徐世昌, 陈怀谷, 刘艳, 高利, 刘博, 郑传临, 陈万权. 中国冬小麦区域试验品种抗病性评价[J]. 中国农业科学, 2015, 48(15):2967-2975.doi:10.3864/j.issn.0578-1752.2015.15.006.
Liu T G, Qiu J, Zhou Y L, Xu S C, Chen H G, Liu Y, Gao L, Liu B, Zheng C L, Chen W Q. Multi-disease resistance evaluation of Chinese advanced winter wheat lines for the national regional test[J]. Scientia Agricultural Sinica, 2015,48(15):2967-2975.
[7] 王吐虹, 郭青云, 蔺瑞明, 姚强, 冯晶, 王凤涛, 陈万权, 徐世昌. 中国40个小麦农家品种和甘肃南部40个生产品种抗条锈病基因推导[J]. 中国农业科学, 2015, 48(19):3834-3847.doi:10.3864/j.issn.0578-1752.2015.19.006.
Wang T H, Guo Q Y, Lin R M, Yao Q, Feng J, Wang F T, Chen W Q, Xu S C. Postulation of stripe rust resistance genes in Chinese 40 wheat landraces and 40 commercial cultivars in the southern region of Gansu Province[J]. Scientia Agricultura Sinica, 2015,48(19):3834-3847.
[8] 王步云, 冯晶, 王凤涛, 蔺瑞明, 陈万权, 徐世昌. 河南省小麦品种(系)遗传多样性和抗条锈性分析[J]. 华北农学报, 2014, 29(S1):62-70.doi:10.7668/hbnxb.2014.S1.015.
Wang B Y, Feng J, Wang F T, Lin R M, Chen W Q, Xu S C. Analysis on genetic diversity and stripe rust resistance of wheat cultivars in Henan[J]. Acta Agriculturae Boreali-Sinica, 2014,29(S1):62-70.
[9] 高晓梅, 冯晶, 蔺瑞明, 王凤涛, 何月秋, 徐世昌. 2008-2009年度河北、河南、四川和江苏四省冬小麦区试品系的遗传多样性和抗条锈性分析[J]. 植物保护, 2014, 40(2):113-118, 140.doi:10.3969/j.issn.0529-1542.2014.02.022.
Gao X M, Feng J, Lin R M, Wang F T, He Y Q, Xu S C. Genetic diversity for wheat regional trial cultivars of Hebei,Henan,Sichuan and Jiangsu provinces in 2018-2019[J]. Plant Protection, 2014,40(2):113-118, 140.
[10] 王步云, 冯晶, 蔺瑞明, 王凤涛, 陈万权, 徐世昌. 2007-2008年度冀鲁豫川四省小麦区试品种(系)遗传多样性和抗条锈性分析[J]. 中国农学通报, 2014, 30(28):255-263.
Wang B Y, Feng J, Lin R M, Wang F T, Chen W Q, Xu S C. Genetic diversity and stripe rust resistance of wheat regional trial cultivars in Hebei,Shandong,Henan and Sichuan in 2007-2008[J]. Chinese Agricultural Science Bulletin, 2014,30(28):255-263.
[11] 曹世勤, 吕小欢, 黄瑾, 冯晶, 张勃, 骆惠生, 贾秋珍, 王晓明, 王万军, 金社林, 孙振宇. 甘肃省26个春小麦品种(系)苗期抗条锈基因分析及成株期抗病性评价[J]. 麦类作物学报, 2013, 33(4):771-776.doi:1009-1041(2013) 04-0771-06.
Cao S Q, Lü X H, Huang J, Feng J, Zhang B, Luo H S, Jia Q Z, Wang X M, Wang W J, Jin S L, Sun Z Y. Postulation of resistant genes at seedling stage and evaluation of adult resistance to stripe rust of 26 spring wheat cultivars(lines)from Gansu Province[J]. Journal of Triticeae Crops, 2013,33(4):771-776.
[12] 曹世勤, 王晓明, 贾秋珍, 孙振宇, 骆惠生, 张勃, 黄瑾, 金明安, 王万军, 金社林. 2003-2013年小麦品种(系)抗条锈性鉴定及评价[J]. 植物遗传资源学报, 2017, 18(2):253-260.doi:10.13430/j.cnki.jpgr.2017.02.011.
Cao S Q, Wang X M, Jia Q Z, Sun Z Y, Luo H S, Zhang B, Huang J, Jin M A, Wang W J, Jin S L. Evaluation of resistance to stripe rust in wheat varieties(lines)during 2003-2013 in Longnan region,Gansu Province[J]. Journal of Plant Genetic Resources, 2017,18(2):253-260.
[13] 李永平, 曹世勤, 金社林, 冯晶, 谭璀榕. 临麦系列春小麦品种抗条锈性分析[J]. 植物保护, 2016, 42(2):209-213.doi:10.3969/j.issn.0529-1542.2016.02.038.
Li Y P, Cao S Q, Jin S L, Feng J, Tan C R. Resistance analysis on spring wheat cultivar of Linmai lines to stripe rust in Linxia,Gansu Province[J]. Plant Protection, 2016,42(2):209-213.
[14] 王万军, 曹世勤, 王晓明, 张立异, 黄瑾, 孙振宇, 张勃, 贾秋珍, 金社林, 张庆勤. 86份贵协系小麦种质资源对条锈病的抗病性评价[J]. 植物保护, 2016, 42(2):198-203.doi:10.3969/j.issn.0529-1542.2016.02.036.
Wang W J, Cao S Q, Wang X M, Zhang L Y, Huang J, Sun Z Y, Zhang B, Jia Q Z, Jin S L, Zhang Q Q. Identification of resistance to stripe rust in 86 Guixie wheat germplasm[J]. Plant Protection, 2016,42(2):198-203.
[15] 周新力, 詹刚明, 黄丽丽, 韩德俊, 康振生. 80份国外春小麦种质资源抗条锈性评价[J]. 中国农业科学, 2015, 48(8):1518-1526.doi:10.3864/j.issn.0578-1752.2015.08.06.
Zhou X L, Zhan G M, Huang L L, Han D J, Kang Z S. Evaluation of resistance to stripe rust in eighty abroad spring wheat germplasms[J]. Scientia Agricultura Sinica, 2015,23(8):1518-1526.
[16] 姚强. 120个春小麦品种(系)成株期抗条锈性鉴定与评价[J]. 西北农业学报, 2014, 23(7):96-101.doi:10.7606/j.issn.1004-1389.2014.07.017.
Yao Q. Identification and evaluation of adult-plant resistance to stripe rust in 120 spring wheat varieties (lines)[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2014,23(7):96-101.
[17] 侯璐, 闫佳会, 姚强, 李秋荣, 马永强, 郭青云. 81份春小麦种质资源抗条锈病评价[J]. 植物保护, 2017, 43(3):171-176.doi:10.3969/j.issn.0529-1542.2017.03.029.
Hou L, Yan J H, Yao Q, Li Q R, Ma Y Q, Guo Q Y. Identification and analysis of 81 spring wheat germplasm resources resistance to stripe rust[J]. Plant Protection, 2017,43(3):171-176.
[18] 侯璐, 姚强, 闫佳会, 郭青云. 402份国内外春小麦种质资源抗条锈性评价及分析[J]. 植物保护学报, 2018, 45(1):181-182.doi:10.13802/j.cnki.zwbhxb.2018.2016100.
Hou L, Yao Q, Yan J H, Guo Q Y. Identification and analysis of 402 worldwide spring wheat germplasm resources resistant to stripe rust[J]. Journal of Plant Protection, 2018, 45(1):181-182.
[19] 侯璐. 4个春小麦种质资源抗条锈性鉴定和抗性遗传分析[J]. 麦类作物学报, 2017, 37(1):80-86.doi:10.7606/j.issn.1009-1041.2017.01.11.
Hou L. Stripe rust resistance gene evaluation and genetic analysis of 4 spring wheat germplasm resources[J]. Journal of Triticeae Crops, 2017, 37(1):80-86.
[20] 侯璐, 闫佳会, 姚强, 李秋荣, 郭青云, 任迎辉, 付丽颖. 4个春小麦种质资源苗期抗条锈基因遗传分析[J]. 西北农业学报, 2018, 27(1):24-30.doi:10.7606/j.issn.1004-1389.2018.01.004.
Hou L, Yan J H, Yao Q, Li Q R, Guo Q Y, Ren Y H, Fu L Y. Stripe rust resistence gene genetic analysis of four spring wheat germplasm resources on seedling stage[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2018,27(1):24-30.
[21] 姚强, 贺苗苗, 闫佳会,郭青云. 春小麦品种青春39的抗条锈性遗传分析[J]. 麦类作物学报, 2014, 34(1):39-42.doi:10.7606/j.issn.1009-1041.2014.01.07.
Yao Q, He M M, Yan J H, Guo Q Y. Genetic analysis of resistance to stripe rust in spring wheat cultivar Qingchun 39[J]. Journal of Triticeae Crops, 2014,34(1):39-42.
[22] 张调喜, 闫佳会, 侯璐, 姚强, 郭青云, 马麟. 春小麦品种墨波成株期抗条锈基因遗传解析[J]. 植物保护学报, 2018, 45(1):60-66.doi:10.13802/j.cnki.zwbhxb.2018.2018906.
Zhang D X, Yan J H, Hou L, Yao Q, Guo Q Y, Ma L. Genetic analysis of spring wheat Mobo stripe rust resistance at adult stage[J]. Acta Phytophylacica Sinica, 2018,45(1):60-66.
[23] 张调喜, 闫佳会, 侯璐, 张贵, 姚强, 马麟, 郭青云. 青海春小麦品种青春38成株期抗条锈性遗传解析[J]. 植物保护, 2018, 44(5):247-252.doi:10.16688/j.zwbh.2017416.
Zhang D X, Yan J H, Hou L, Zhang G, Yao Q, Ma L, Guo Q Y. Genetic analysis of adult plant resistance to stripe rust in Qinghai spring wheat cultivar Qingchun 38[J].Plant Protection, 2018,44(5):247-252.
[24] Hou L, Chen X M, Wang M N, See Deven R, Chao S M, Bulli Peter, Jing J X. Mapping a large number of QTL for durable resistance to stripe rust in winter wheat druchamp using SSR and SNP markers[J]. PLoS One, 2015, 10(5):e0126794.doi:10.1371/journal.pone.0126794.
[25] 刘兵. 植物数量性状分离分析Windows软件包SEA的研制[D]. 南京:南京农业大学, 2013.
Liu B. A software package of segregation analysis of quantitative traits in plants[D].Nanjing:Nanjing Agricultural University, 2013.
[26] 刘青元. 青海省农作物品种志(1983-2005)[M]. 西宁:青海人民出版社, 2008:32.
Liu Q Y. Crop variety of Qinghai Province(1983-2005)[M]. Xining:Qinghai People's Publishing House,2008:32.
[27] 张莹, 周新力, 王琪琳, 韩德俊, 黄丽丽, 康振生, 李海峰. 小麦品系P9897成株期抗条锈性遗传分析[J]. 麦类作物学报, 2015, 35(10):1355-1359.doi:10.7606/j.issn.1009-1041.2015.10.05.
Zhang Y, Zhou X L, Wang Q L, Han D J, Huang L L, Kang Z S, Li H F. Genetic analysis of adult-plant resistance to stripe rust in wheat line P9897[J]. Journal of Triticeae Crops, 2015,35(10):1355-1359.
[28] 周春宏, 徐智斌, 冯波, 项超, 王涛. 西藏地方小麦品种曲白春的抗条锈性遗传分析[J]. 麦类作物学报, 2015, 35(3):312-316.doi:10.7606/j.issn.1009-1041.2015.03.04.
Zhou C H, Xu Z B, Feng B, Xiang C, Wang T. Genetic analysis of stripe rust resistance in Tibetan wheat landrace Qubai Chun[J]. Journal of Triticeae Crops, 2015,35(3):312-316.
[29] 马东方, 周新力, 井金学, 王文立, 王岭岭. 小偃6号成株期高温抗条锈性遗传分析[J]. 植物保护学报, 2013, 40(1):33-37.doi:10.13802/j.cnki.zwbhxb.2013.01.020.
Ma D F, Zhou X L, Jing J X,Wang W L, Wang L L. Inheritance analysis of adult plant high-temperature stripe rust resistance in wheat cultivar Xiaoyan 6[J]. Journal of Plant Protection, 2013,40(1):33-37.
[30] 宁利园, 郄彦敏, 王凤涛, 冯晶, 蔺瑞明, 徐世昌. 中国小麦农家品种红锁条和白蚂蚱的抗条锈性遗传分析[J]. 植物保护学报, 2015, 42(2):145-152.doi:10.13802/j.cnki.zwbhxb.2015.02.001.
Ning L Y, Xi Y M, Wang F T, Feng J, Lin R M, Xu S C. Genetic analysis on the resistance of Chinese wheat landraces Hongsuotiao and Baimazha to stripe rust pathogen[J]. Acta Phytophylacica Sinica, 2015,42(2):145-152.
[31] 苏萍萍, 曾庆东, 李海洋, 王琪琳, 吴建辉, 穆京妹, 康振生, 韩德俊. 小麦种质P10078的成株期条锈病抗性特征及遗传规律[J]. 麦类作物学报, 2017, 16(4):500-503.doi:10.7606/j.issn.1009-1041.2017.04.10.
Su P P, Zeng Q D, Li H Y, Wang Q L, Wu J H, Mu J M, Kang Z S, Han D J. Characterization of resistance to stripe rust and inheritance at adult stage in wheat germplasm P10078[J]. Journal of Triticeae Crops, 2017,16(4):500-503.

选择文件类型/文献管理软件名称

选择包含的内容