摘要： 为了解宁夏春小麦的品质遗传基础,应用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)技术,对33个普通小麦品种(包括地方品种、育成品种和引进品种)HMW-GS构成、不同等位基因间亚基变异及出现频率系统分析,并计算了品质得分。结果表明,宁夏春小麦品种HMW-GS等位基因Glu-A1编码1、1*、2*、null 4种亚基,Glu-B1编码7、6+8、7+8、7+9、17+18 5种,Glu-D1编码2+12、5+10 2种亚基,频率最高的亚基分别是1(69.70%)、7(36.36%)、5+10(84.85%);参试材料的品质评分为6～10,平均分为8.42分;优质亚基组成类型如1、7+8、5+10,1、7+9、5+10在育成品种中出现频率较高。
关键词:
春小麦,
高分子量麦谷蛋白亚基,
SDS-PAGE
Abstract: In order to understand the genetic diversity of wheat germplasms in Ningxia,High-molecular-weight glutenin subunit(HMW-GS) composition of 33 spring wheat varieties were determined by sodium do-decylsulphate polyacrylamide gel electrophoresis(SDS-PAGE).The results revealed:Glu-A1 encoded four subunits of 1,1*,2*and null,Glu-B1 encoded five subunits of 7,6+8,7+8,7+9,17+18,Glu-D1 encoded two subunits of 2+12 and 5+10.The subunit with the highest frequency is 1(69.70%),7(36.360%),5+10(84.85%) in Glu-A1,Glu-B1 and Glu-D1 respectively.According to the standard grades of Payne,the most varieties Glu-1 quality scores was 6-10,average 8.42.Compared on HMW-GS composition of three-source spring wheat varieties,modern varieties had high frequencies of quality subunits Composition 1,7+8,5+10 and 1,7+9,5+10.
Key words:
Spring wheat,
HMW-GS,
SDS-PAGE
中图分类号:
赵海霞, 任贤, 王洋, 贝盏临. 宁夏部分春小麦种(系)高分子量麦谷蛋白亚基组成分析[J]. 华北农学报, 2011, 26(S1): 56-59. doi: 10.7668/hbnxb.2011.S1.012.
ZHAO Hai-xia, REN Xian, WANG Yang, BEI Zhan-lin. Composition of High Molecular Weight Glutenin Subunits of Spring Wheat Varieties in Ningxia[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S1): 56-59. doi: 10.7668/hbnxb.2011.S1.012.