摘要： 为揭示向日葵抗铅胁迫的分子机理,采用傅立叶红外光谱分析法和双向电泳法探讨向日葵试材HA89的幼苗在不同浓度(0,400,800 mg/L)铅胁迫条件下蛋白质水平发生的变化。结果表明:向日葵幼苗根、茎、叶的蛋白质二级结构中的β-折叠结构和无规则卷曲结构对铅胁迫最为敏感,同时,根据1655 cm^-1/1637 cm^-1比值可看出向日葵幼苗蛋白在铅胁迫浓度为400 mg/L时蛋白质二级结构较为稳定,超过这一浓度到800 mg/L时,蛋白二级结构则趋于松散;试验采用Tris酚提取法提取试验材料全蛋白,经双向电泳分析后发现差异蛋白点9个。经双向电泳分析后匹配完全相同蛋白点数65对,差异倍数为2倍以上的9个,根据蛋白点的等电点和分子量对照蛋白库对比后,推测变化较为显著的蛋白是与感应有关的激酶。

关键词: 向日葵幼苗, 铅胁迫, 傅立叶红外光谱, SDS-PAGE电泳, 双向电泳

Abstract: In order to explore the molecular mechanism of plumbum tolerance in sunflower, the alterations of sunflower seedlings in protein level under different concentrations of lead stress (0, 400, 800 mg/L), had been analyzed by FTIR infrared spectrometry and two-dimensional electrophoresis.The results showed that the irregular structurs structure in proteins from the roots, stems, and leaves of sunflower seedlings was significantly sensitive to lead stress compared with other structures.Measurements on the ratio of 1 655 cm^-1/1 637 cm^-1 under lead stress indicated that secondary structures of proteins were relatively stable under 400 mg/L of lead solution, while the structures were loosely organized under 800 mg/L.The total proteins of materials mentioned above were extracted by Tris-phenol extraction method.Then 65 same protein spots were found by two-dimensional electrophoresis analysis in comparison with control treatment, more than 9 spots were differences in two times multiples.According to the protein isoelectric and molecular mass to protein databank, speculated that change is more significant with kinase.

Key words: Sunflower seedlings, Lead stress, FTIR, SDS-PAGE electrophoresis, Two-dimensional electrophoresis

中图分类号: 

• S565.03