摘要： 以高抗大豆胞囊线虫3号生理小种品种灰皮支黑豆(ZDD2315)为父本,高感大豆胞囊线虫3号生理小种品种辽豆15为母本,配制杂交组合,利用分离群体分组分析法将杂交后代分为抗池和感池。采用三氯乙酸/丙酮法提取大豆根系总蛋白质,运用双向电泳和质谱分析技术研究大豆胞囊线虫3号生理小种胁迫下不同抗性大豆杂交后代根系蛋白质组的变化。结果表明:抗池、感池分别有367,372个蛋白点在银染的2-D胶上分离,经ImageMaster 2D Platinum software对2-D胶分析后,以感池为参考胶,抗池有6个蛋白点特异表达,13个蛋白点的含量上调2倍以上,4个蛋白点的含量下调2倍以上,感池有4个蛋白点特异表达。对以上27个差异蛋白点进行MALDI-TOF-MS质谱分析,共鉴定出16个蛋白点,11个蛋白点因匹配率太低未得到鉴定。

关键词: 大豆, 大豆胞囊线虫, 双向电泳, 质谱分析

Abstract: The cross was made between resistant variety Huipizhi Heidou ( ZDD2315 ) and susceptible variety Liaodou 15 to Heterodera glycines race 3. Applying the F 4 separated population from the cross of Huipizhi Heidou and Liaodou 15 as test materials according to Bulked segregant analysis ( BSA ) to study differential proteomic. The total proteins were extracted by TCA/acetone,two-dimensional gel electrophoresis and mass spectrometry were em-ployed to analyze proteins extracted from roots of soybean differentially expressed induced by Heterodera glycines. The results showed that there were 367 and 372 protein spots isolated in silver nitrate stained 2-D gels in resistant and sensitive samples,the 2-D gels were analyzed by ImageMaster 2D Platinum software,there were 6 special pro-tein spots,13 twofold upper protein spots,4 twofold lower protein spots in resistant samples,4 special protein spots in sensitve samples. 16 protein spots were identified by MALDI-TOF-MS and blast in NCBI, and 7 protein spots were from soybean,11 protein spots weren't identified due to the low scores.

Key words: Soybean, Heterodera glycines, Two-dimensional gel electrophoresis, Mass spectrometry

中图分类号: 

• S435.651