大豆新品系抗SMV鉴定及分析

doi:10.7668/hbnxb.20191757

摘要/Abstract

摘要： 为了筛选出对大豆花叶病毒（SMV）病具有抗性的大豆新品系，以1 108份新选育的大豆品系为材料，人工接种SMV优势株系SC3和SC7，通过调查发病率和病情指数以考察其抗性，同时分析其亲本来源和抗性。结果显示，有356和326份大豆品系对SMV株系SC3和SC7分别表现高抗，占比为32.13%，29.42%，同时对这2个株系均表现高抗的品系有252份，占22.75%。相关性分析结果显示，SC3和SC7发病率与病情指数呈极显著正相关（r=0.942和r=0.956）。线性回归结果显示，SC3和SC7发病率与其病情指数呈线性关系，线性关系显著，其发病率可以分别解释其病情指数变异的88.75%，91.47%。从亲本来源可以看到，母本来源于河北育成的品系数最多，有97份；父本来源于国外育成的品系数最多，为85份。抗性分析显示，来源于河北的母本抗性的病情指数相对较低，其后代品系平均发病率和病指指数也均相对较低。研究结果为SMV病情预测和大豆抗病育种提供了参考。

关键词: 大豆, 品系, 大豆花叶病毒, 亲本来源, 抗性分析

Abstract: In order to screen soybean lines with resistance to Soybean mosaic virus(SMV), a total of 1 108 recent bred soybean lines were evaluated through the incidence rate and disease index to SMV by inoculation with two SMV prevalent strains SC3 and SC7. The results showed that among the 1 108 varieties inoculated by SC3 and SC7, 356(32.13%)and 326(29.42%)exhibited high resistance, respectively. 252(22.75%)varieties were high resistance to both SC3 and SC7. The correlation analysis showed the incidence rate to SC3 and SC7 had extremely significantly positive relation with disease index(r=0.942 and r=0.956). The linearity regression analysis indicated, the incidence rate to SC3 and SC7 if only disease index to become the linear relations, the linear relations quite was remarkable. The incidence rate of SC3 and SC7 could explain 88.75% and 91.47% of the disease index variation, respectively. The analysis of the parental origins showed that the new lines was the most, were bred by the female from Hebei, account for 97, while the new lines was the most, were bred by the male from foreign varieties, account for 85. The resistance analysis showed that the average incidence rate and disease index to SC3 and SC7 of the offspring lines that were bred by the female from Hebei were relatively lower. These findings could provide reference for the prediction of Soybean mosaic virus disease and SMV resistance breeding.

Key words: Soybean, Lines, Soybean mosaic virus, Parental origins, Resistance analysis

中图分类号:

S565.03

王大刚, 陈圣男, 黄志平, 李杰坤, 吴倩, 胡国玉, 王维虎, 杨永庆. 大豆新品系抗SMV鉴定及分析[J]. 华北农学报, 2021, 36(S1): 326-332. doi: 10.7668/hbnxb.20191757.

WANG Dagang, CHEN Shengnan, HUANG Zhiping, LI Jiekun, WU Qian, HU Guoyu, WANG Weihu, YANG Yongqing. Identification and Analysis of Soybean New Lines for Resistance to Soybean mosaic virus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 326-332. doi: 10.7668/hbnxb.20191757.

http://www.hbnxb.net/CN/Y2021/V36/IS1/326

参考文献

[1] 智海剑,盖钧镒,陈应志,廖琴,郭东全,王延伟,李凯,李海潮. 2002-2004年国家大豆区试品种对大豆花叶病毒抗性的评价[J].大豆科学,2005,24(3):189-194.doi:10.3969/j.issn.1000-9841.2005.03.006.
Zhi H J,Gai J Y,Chen Y Z,Liao Q,Guo D Q,Wang Y W,Li K,Li H C. Evaluation of resistance to SMV of the entries in the national uniform soybean tests(2002-2004)[J]. Soybean Science,2005,24(3):189-194.
[2] 白丽,李凯,陈应志,智海剑,盖钧镒,杨清华,杨华,李海朝. 部分国家和省(市)区试品种对大豆花叶病毒的抗性分析[J].中国油料作物学报,2007,29(1):86-89.doi:10.3321/j.issn:1007-9084.2007.01.018.
Bai L,Li K,Chen Y Z,Zhi H J,Gai J Y,Yang Q H,Yang H,Li H C.Evaluation of resistance to SMV of cultivars from soybean national and local regional test[J]. Chinese Journal of Oil Crop Sciences,2007,29(1):86-89.
[3] 杨华,李凯,杨清华,王大刚,刘宁,马莹,智海剑. 国内部分新品种对大豆花叶病毒抗性的鉴定[J].华北农学报,2008,23(S2):252-255.doi:10.7668/hbnxb.2008.S2.058.
Yang H,Li K,Yang Q H,Wang D G,Liu N,Ma Y,Zhi H J. Evaluation of resistance to SMV of cultivars from soybean national and local regional test in 2004-2006[J]. Acta Agriculturae Boreali-Sinica,2008,23(S2):252-255.
[4] 王大刚,卢为国,马莹,刘宁,陈珊宇,郑桂杰,杨中路,刘若淼,智海剑. 新育成大豆品种对SMV和SCN的抗性评价[J].大豆科学,2009,28(6):949-953.doi:10.11861/j.issn.1000-9841.2009.06.0949.
Wang D G,Lu W G,Ma Y,Liu N,Chen S Y,Zheng G J,Yang Z L,Liu R M,Zhi H J. Evaluation of resistance of soybean cultivars to Soybean mosaic virus and Soybean cyst nematode[J]. Soybean Science,2009,28(6):949-953.
[5] 李凯,刘志涛,李海朝,张锴,王成坤,任锐,卢为国,智海剑. 国家大豆区域试验品种对SMV和SCN的抗性分析[J].大豆科学,2013,32(5):670-675.
Li K,Liu Z T,Li H C,Zhang K,Wang C K,Ren R,Lu W G,Zhi H J. Resistance to Soybean mosaic virus and Soybean cyst nematode of soybean cultivars from China national soybean uniform trials[J]. Soybean Science,2013,32(5):670-675.
[6] 侯文焕,杨永庆,林静,张孟臣,智海剑,杨春燕. 不同来源大豆材料对SMV株系SC3和SC7的抗性分析[J].大豆科学,2015,34(5):861-866.doi:10.11861/j.issn.1000-9841.2015.05.0861.
Hou W H,Yang Y Q,Lin J,Zhang M C,Zhi H J,Yang C Y. Resistance of different origin soybeans to SMV strains SC3 and SC7[J]. Soybean Science,2015,34(5):861-866.
[7] 于国宜,王大刚,吴倩,胡晨,胡国玉,李杰坤,黄志平. 2009-2015年安徽省大豆试验新品系对SMV和SCN的抗性评价[J].大豆科学,2016,35(5):782-788.doi:10.11861/j.issn.1000-9841.2016.05.0782.
Yu G Y,Wang D G,Wu Q,Hu C,Hu G Y,Li J K,Huang Z P. Evaluation of resistance of soybean new lines to Soybean mosaic virus and Soybean cyst nematode in Anhui Province in 2009-2015[J]. Soybean Science,2016,35(5):782-788.
[8] 黄志平,李杰坤,王维虎,胡国玉,吴倩,王大刚. 大豆新品系抗SMV鉴定及其抗性来源分析[J].大豆科学,2017,36(4):598-605.doi:10.11861/j.issn.1000-9841.2017.04.0598.
Huang Z P,Li J K,Wang W H,Hu G Y,Wu Q,Wang D G. Identification of resistance and preliminary analysis of resistance sources for the Soybean mosaic virus in new soybean lines[J]. Soybean Science,2017,36(4):598-605.
[9] 王大刚,陈圣男,李杰坤,吴倩,胡国玉,黄志平. 大豆品系抗SMV评价及亲本来源分析[J].大豆科学,2018,37(5):657-663.doi:10.11861/j.issn.1000-9841.2018.05.0657.
Wang D G,Chen S N,Li J K,Wu J,Hu G Y,Huang Z P.Evaluation of resistance and analysis of parental origins for soybean lines to Soybean mosaic virus[J]. Soybean Science,2018,37(5):657-663.
[10] 王大刚,陈圣男,黄志平,吴倩,胡国玉,李杰坤. 193份大豆品系对SMV抗性鉴定与分子标记检测[J].分子植物育种,2019,17(24):8138-8151.doi:10.13271/j.mpb.017.008138.
Wang D G,Chen S N,Huang Z P,Wu Q,Hu G Y,Li J K. Identification and molecular detection of Soybean mosaic virus resistance of 193 soybean lines[J]. Molecular Plant Breeding,2019,17(24):8138-8151.
[11] 王树峰,卢为国,李卫东. 河南省大豆审定品种系谱来源及性状分析[J].河南农业科学,2015,44(1):37-41.doi:10.15933/j.cnki.1004-3268.2015.01.009.
Wang S F,Lu W G,Li W D. Pedigree and trait analysis of soybean varieties registered in Henan Province[J]. Journal of Henan Agricultural Sciences,2015,44(1):37-41.
[12] 姚清鉴. 玉米新选育品系的抗病性表型鉴定及抗性来源初步分析[D].北京:中国农业科学院,2013.
Yao Q J. Phenotypic identification of resistance and preliminary analysis of resistance source for the disease in the new maize lines[D].Beijing:Chinese Academy of Agricultural Sciences,2013.
[13] 王修强,盖钧镒,濮祖芹. 黄淮和长江中下游地区大豆花叶病毒株系鉴定与分布[J].大豆科学,2003,22(2):102-107.doi:10.3969/j.issn.1000-9841.2003.02.006.
Wang X Q,Gai J Y,Pu Z Q. Classification and distribution of strain groups of Soybean mosaic virus in middle and lower Huang-Huai and Changjiang valleys[J]. Soybean Science,2003,22(2):102-107.
[14] 智海剑,盖钧镒,何小红. 大豆对SMV数量(程度)抗性的综合分级方法研究[J].大豆科学,2005,24(1):5-11.doi:10.3969/j.issn.1000-9841.2005.01.002.
Zhi H J,Gai J Y,He X H. Study on methods of classification of quantitative resistance to Soybean mosaic virus in soybean[J]. Soybean Science,2005,24(1):5-11.
[15] 王大刚,黄志平,张磊,李杰坤,吴倩,胡国玉. 大豆抗大豆花叶病毒病鉴定技术规程[S].安徽省质量技术监督局,DB34/T 2510-2015.
Wang D G,Huang Z P,Zhang L,Li J K,Wu Q,Hu G Y. Rules for evaluation of soybean for resistance to Soybean mosaic virus[S].Anhui Bureau of Quality and Technical Supervision,DB34/T 2510-2015.
[16] Zhi H J,Gai J Y. Performances and germplasm evaluation of quantitative resistance to Soybean mosaic virus in soybeans[J]. Agricultural Science in China,2004,3(4):247-253.
[17] 王大刚,胡国玉,黄志平,张磊,于国宜. 大豆种质资源抗大豆花叶病毒的鉴定[J].作物杂志,2013(2):32-36.doi:10.16035/j.issn.1001-7283.2013.02.027.
Wang D G,Hu G Y,Huang Z P,Zhang L,Yu G Y. Identification of soybean germplasm resources resistance to Soybean mosaic virus[J]. Crops,2013(2):32-36.
[18] 易自力,徐乃瑜. 不同山羊草细胞质效应的比较研究[J].草业学报,2000,9(1):73-78.doi:10.3321/j.issn:1004-5759.2000.01.012.
Yi Z L,Xu N Y. Comparative studies in cytoplasmic effects of Aegilops species[J]. Acta Prataculturae Sinica,2000,9(1):73-78.
[19] Wang Q F,Yi Q,Hu Q Q,Zhao Y,Nian H J,Li K Z,Yu Y X,Izui K,Chen L M. Simultaneous overexpression of citrate synthase and phosphoenolpyruvate carboxylase in leaves augments citrate exclusion and Al resistance in transgenic tobacco[J]. Plant Molecular Biology Reporter,2012,30(4):992-1005.doi:10.1007/s11105-011-0397-z.
[20] 张匀华. 小麦赤霉病发病率与病情指数关系初步研究[J].黑龙江农业科学,2000(3):3-5.doi:10.3969/j.issn.1002-2767.2000.03.002.
Zhang Y H. The relation between incidence and disease severity of wheat scab[J]. Heilongjiang Agricultural Science,2000(3):3-5.

选择文件类型/文献管理软件名称

选择包含的内容