RNA-Seq技术鉴定大豆雄性不育候选基因

doi:10.7668/hbnxb.20196210

华北农学报 ›› 2026, Vol. 41 ›› Issue (2): 1-11. doi: 10.7668/hbnxb.20196210

所属专题： 油料作物；生物技术；

• 作物遗传育种·种质资源·生物技术 • 下一篇

RNA-Seq技术鉴定大豆雄性不育候选基因

陈天宇¹^,²^,³, 徐超¹, 赵亮¹, 王子烨¹, 张琦¹^,²^,³^,⁴, 杜吉到¹^,²^,³^,⁴

¹ 黑龙江八一农垦大学农学院, 黑龙江大庆 163319
² 农业农村部东北平原农业绿色低碳重点实验室, 黑龙江大庆 163319
³ 黑龙江省盐碱地改良工程技术研究中心, 黑龙江大庆 163319
⁴ 国家杂粮工程技术研究中心, 黑龙江大庆 163319

收稿日期:2025-06-14 出版日期:2026-05-06
通讯作者:
张琦(1994—),男,黑龙江大庆人,副教授,博士,主要从事杂豆种质资源收集鉴定及耐盐生理和耐盐碱基因挖掘研究。
杜吉到(1973—),男,黑龙江大庆人,教授,博士,主要从事作物耐盐碱种质资源鉴定、品种选育及耐盐碱栽培技术研究。
作者简介:
陈天宇(2000—),男,黑龙江大庆人,硕士,主要从事作物种质资源创新研究。
基金资助:
黑龙江省“双一流”新一轮建设学科协同创新成果建设项目“粮食作物绿色低碳”(LJGXCG2022-107); 黑龙江省“双一流”新一轮建设学科协同创新成果建设项目(LJGXCG2022-111)

Identification of Candidate Genes for Male Sterile in Soybean by RNA-Seq

CHEN Tianyu¹^,²^,³, XU Chao¹, ZHAO Liang¹, WANG Ziye¹, ZHANG Qi¹^,²^,³^,⁴, DU Jidao¹^,²^,³^,⁴

¹ College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, China
² Key Laboratory of Low-Carbon Green Agriculture in Northeastern China, Ministry of Agriculture and Rural Affairs P.R.China, Daqing 163319, China
³ Heilongjiang Salt Alkali Land Improvement Engineering Technology Research Center, Daqing 163319, China
⁴ National Grain Engineering Technology Research Center, Daqing 163319, China

Received:2025-06-14 Published:2026-05-06

摘要/Abstract

摘要：

大规模生产大豆杂交种,利用杂种优势是种质资源创新与品种改良关键所在。通过挖掘和利用大豆雄性不育基因,能够培育出更具优势的大豆品种,从而提升大豆的产量和品质。选用宝泉岭绿大豆(母本)与黑农44(父本)杂交后代出现的雄性不育突变群体作为试验材料。选取大豆可育植株与不育植株的花蕾,对花药与花粉进行细胞学观察,并采用高通量测序平台对大豆可育植株与不育植株的花与花药组织进行转录组测序,对测序结果进行注释、筛选与分析。结果表明,不育植株的绒毡层发育异常,花粉高度不育,是导致雄性不育的重要原因。戊糖和葡萄糖醛酸相互转化途径(ko00040:Pentose and glucuronate interconversions)是影响大豆育性的关键通路,编码果胶甲酯酶(PEM)与果胶裂解酶(PL)基因呈下调表达。差异表达基因蛋白质互作网络表明,果胶裂解酶基因是影响戊糖和葡萄糖醛酸相互转化途径的关键基因。通过对果胶裂解酶含量的测定表明,不育植物花药中果胶裂解酶含量显著降低。qRT-PCR检测结果与RNA-Seq测序结果一致。推测这些基因的下调表达可能引起果胶裂解酶活性下降,从而影响绒毡层发育,导致雄性不育。

关键词: 大豆, 雄性不育, 转录组学, 绒毡层, 果胶裂解酶

Abstract:

The large-scale production of soybean hybrids and the utilization of hybrid vigor are key to germplasm resource innovation and variety improvement.By mining and utilizing soybean male sterility genes,more advantageous soybean varieties can be cultivated,thereby improving soybean yield and quality.This study selected the male sterile mutant population that appeared in the hybrid offspring of Baoquanling green soybean(female parent)and Heinong 44(male parent)as the experimental material.Flower buds were selected from soybean fertile and sterile plants,the anthers and pollen cytology were observed,and a high-throughput sequencing platform was used to perform transcriptome sequencing on the flower and anther tissues of soybean fertile and sterile plants.Annotated,screened and analyzed the sequencing results.The results indicated that abnormal development of the tapetum layer and high pollen sterility in sterile plants were important causes of male infertility.The pentose and glucuronate interconversion pathway(ko00040:Pentose and glucuronate interconversion)was a key pathway affecting soybean fertility,and the genes encoding pectin methylesterase(PEM)and pectin lyase(PL)were downregulated in expression.The differentially expressed gene protein interaction network indicated that the pectin lyase gene was a key gene affecting the interconversion pathway between pentose and glucuronic acid.The determination of pectin lyase content showed that the pectin lyase content in sterile plant anthers was significantly reduced.The qRT-PCR detection results were consistent with the RNA-Seq sequencing results.It is speculated that the downregulation of these genes may cause a decrease in pectin lyase activity,thereby affecting the development of the tapetum and leading to male infertility.

Key words: Soybean, Male infertility, Transcriptomics, Tapetum, Pectate lyase

中图分类号:

陈天宇, 徐超, 赵亮, 王子烨, 张琦, 杜吉到. RNA-Seq技术鉴定大豆雄性不育候选基因[J]. 华北农学报, 2026, 41(2): 1-11. doi: 10.7668/hbnxb.20196210.

CHEN Tianyu, XU Chao, ZHAO Liang, WANG Ziye, ZHANG Qi, DU Jidao. Identification of Candidate Genes for Male Sterile in Soybean by RNA-Seq[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(2): 1-11. doi: 10.7668/hbnxb.20196210.

http://www.hbnxb.net/CN/Y2026/V41/I2/1

图/表 10

表1 qRT-PCR相应的引物

Tab.1 The corresponding primers of qRT-PCR

基因ID Gene ID	正向引物序列(5'—3') Forward primer(5'—3')	反向引物序列(5'—3') Reverse primer(5'—3')
GmEF1-α	TGCAAAGGAGGCTGCTAACT	CAGCATCACCCTTCTTCAAA
Glyma.01G042700	CACCGGAGCAAGAGTGGA	TGCCTTGAGAACGGCCTG
Glyma.02G020600	GAACCGGACCCTTGTGGA	ATACCAGCCCCACCAGCA
Glyma.04G173900	TGCCAAGGTGCAGATGGG	TAAACCGGTTGCCCTGGC
Glyma.07G150200	ATTCAAGGCGCGCTTTGC	CGTGAGGACCAAGGCCAT
Glyma.08G296900	TGCAATCTGGGCCTGAGC	GCGACCCACTCTGCACTT
Glyma.13G064700	TGCCAAGGTGCAGATGGG	GGCGTTGTCGTTGTTCGG
Glyma.18G255900	TGCCAAGGTGCAGATGGG	GGCAGCGTTGTTGTTGGG
Glyma.19G020200	TTCGGAGCCAGCAACGTT	GAGCCCTGGATGACGTCG
Glyma.20G240800	ATGGCGGGGATGCAGAAG	AGAGAGGACCGTGGAGGC

表2 不育系后代性状分离数量统计

Tab.2 Statistics on the number of trait segregation in the offspring of sterile lines

单株编号 Number	可育植株 (实际值) Fertile	不育植株 (实际值) Sterile	总株数 Total number of plants	卡方值 (单株) χ²	组别 Group	未分离株行数 (实际值) Unseparated plant line	分离株行数 (实际值) Separated plant line	总株行数 Total number of plants and rows	卡方值 (单组) χ²
1	38	13	51	0.006 5	F2-1	7	13	20	0.021 6
2	33	10	43	0.069 6	F2-2	6	14	20	0.108 4
3	34	14	48	0.444 4	F2-3	7	13	20	0.021 6
4	45	14	59	0.051 0	F2-4	7	13	20	0.021 6
5	43	15	58	0.022 9	F2-5	6	14	20	0.108 4
6	35	13	48	0.111 1	F2-6	5	15	20	0.541 6
7	48	15	63	0.047 7	F2-7	7	13	20	0.021 6
8	38	12	50	0.026 7	F2-8	8	12	20	0.433 4
9	41	13	54	0.026 7	F2-9	7	13	20	0.021 6
10	42	15	57	0.052 6	F2-10	6	14	20	0.108 4
总计Total	397	134	534	0.859 2	总计	66	134	200	1.373 4

图1 大豆可育植株与不育植株的表型鉴定 A、B.大豆结荚时期可育植株与不育植株;C、D.大豆可育植株与不育植株花药;E、F.大豆可育植株与不育植株花粉;G、H.大豆可育植株与不育植株花药。

Fig.1 Phenotypic identification of soybean fertile and sterile plants A,B.Feasible and sterile plants during soybean pod setting period;C,D.Soybean fertile plants and sterile plants anthers;E,F.Soybean pollen from fertile and sterile plants;G,H.Soybean fertile plants and sterile plants anthers.

表3 转录组测序数据信息

Tab.3 Transcriptome sequencing data information

样品 Sample	Reads总数 Reads sum	总碱基数 Base sum	GC/%	N/%	Q20/%	Q30/%
FA1	22 843 955	6 820 359 173	44.16	0.04	98.15	94.58
FA2	20 990 838	6 267 855 523	44.09	0.03	97.96	94.14
FA3	21 102 342	6 293 914 777	43.96	0.03	98.04	94.28
FF1	20 283 651	6 052 402 966	43.89	0.03	97.95	94.15
FF2	20 693 432	6 171 776 753	43.89	0.03	97.97	94.20
FF3	22 807 796	6 817 058 932	43.86	0.02	97.75	93.56
SA1	20 907 079	6 242 338 350	43.79	0.03	97.86	93.90
SA2	21 282 893	6 357 207 698	43.79	0.03	97.93	94.01
SA3	21 481 838	6 419 512 460	43.71	0.02	97.95	94.11
SF1	21 556 089	6 444 548 048	43.72	0.02	97.84	93.84
SF2	22 528 378	6 731 511 923	43.58	0.02	97.82	93.70
SF3	20 992 211	6 266 325 309	43.72	0.03	97.85	93.78

图2 差异基因集数目统计与基因共表达趋势

Fig.2 DEGs sets and gene co-expression trend

图3 差异表达基因 KEGG通路富集分析 A.FA vs SA处理的KEGG富集气泡图;B.FF vs SF处理的KEGG富集气泡图;C、D.FA vs SA处理与FF vs SF处理的差异表达基因与KEGG通路的网络图。A:1.戊糖与葡萄糖相互转化,2.植物-病原体相互作用,3.胞吞作用,4.昼夜节律-植物,5.磷脂酰肌醇信号系统,6.甘油磷酸代谢,7.糖酵解/糖异生作用,8.鞘脂代谢,9.亚油酸代谢,10.光合作用生物钟的碳固定过程,11.糖鞘脂生物合成-葡聚糖系列,12.氨基糖与核苷酸糖代谢,13.肌醇磷酸代谢,14.糖胺聚糖降解,15.花青素生物合成,16.果糖与甘露糖代谢,17.半胱氨酸与甲硫氨酸代谢,18.脂肪酸延长作用,19.吞噬体,20.半乳糖代谢;B:1.戊糖与葡萄糖相互转化,2.鲨烯酸代谢,3.甘油磷酸代谢,4.植物-病原体相互作用,5.α-亚麻酸代谢,6.缬氨酸、亮氨酸和异亮氨酸的分解过程,7.胞吞作用,8.甘油酯代谢,9.果糖与甘露糖代谢,10.乙醚脂质代谢,11.亚油酸代谢,12.光合作用生物钟的碳固定过程,13.氨基糖与核苷酸糖代谢,14.糖酵解/糖异生作用,15.氧化磷酸化,16.吞噬体,17.肌醇磷酸代谢,18.异喹啉生物碱合成过程,19.鞘脂代谢,20.磷脂酰肌醇信号系统。

Fig.3 KEGG enrichment analysis of differentially expressed genes A.The KEGG enrichment bubble plot for FA vs SA treatment;B.The KEGG enrichment bubble plot for FF vs SF treatment;C,D.The network diagram of differentially expressed genes and KEGG pathway in FA vs SA treatment and FF vs SF treatment,respectively.A:1.Pentose and glucuronate interconversions,2.Plant-pathogen interaction,3.Endocytosis,4.Circadian rhythm-plant,5.Phosphatidylinositol signaling system,6.Glycerophospholipid metabolism,7.Glycolysis/Gluconeogenesis,8.Sphingolipid metabolism,9.Linoleic acid metabolism,10.Carbon fixation in photosynthetic organisms,11.Glycosphingolipid biosynthesis-ganglio series,12.Amino sugar and nucleotide sugar metabolism,13.Inositol phosphate metabolism,14.Glycosaminoglycan degradation,15.Anthocyanin biosynthesis,16.Fructose and mannose metabolism,17.Cysteine and methionine metabolism,18.Fatty acid elongation,19.Phagosome,20.Galactose metabolism;B:1.Pentose and glucuronate interconversions,2.Arachidonic acid metabolism,3.Glycerophospholipid metabolism,4.Plant-pathogen interaction,5.Alpha-linolenic acid metabolism,6.Valine,leucine and isoleucine degradation,7.Endocytosis,8.Glycerolipid metabolism,9.Fructose and mannose metabolism,10.Ether lipid metabolism,11.Linoleic acid metabolism,12.Carbon fixation in photosynthetic organisms,13.Amino sugar and nucleotide sugar metabolism,14.Glycolysis/Gluconeogenesis,15.Oxidative phosphorylation,16.Phagosome,17.Inositol phosphate metabolism,18.Isoquinoline alkaloid biosynthesis,19.Sphingolipid metabolism,20.Phosphatidylinositol signaling system.

图4 差异表达基因GO富集分析 A—C.FA vs SA处理的BP、CC、MF;D—F.FF vs SF处理的BP、CC、MF。A:1.果胶分解过程,2.细胞壁修饰,3.pH值调节,4.单价无机阳离子转运,5.囊泡蛋白组装,6.花粉管生长,7.细胞膜囊泡形成,8.胞吞作用,9.囊泡依赖性胞吞作用,10.细胞内信号转导,11.木聚糖代谢过程,12.细胞壁结构,13.细胞壁生成过程,14.碳水化合物转运,15.Rab 信号转导,16.细胞尖端生长,17.碳水化合物代谢过程,18.细胞发育性生长,19.多细胞生物生长过程,20.授粉过程;B:1.质膜,2.细胞外区域,3.细胞壁,4.被蛋白包裹的囊泡,5.囊泡壳层,6.内膜系统,7.肌动蛋白细胞骨架,8.细胞质,9.单片式复合体,10.细胞顶端部分,11.顶端质膜,12.花粉管,13.高尔基体,14.质膜区域,15.线粒体内膜组成部分,16.细胞骨架,17.液泡质子转运V型ATP酶复合体,18.细胞膜组成部分,19.液泡,20.淀粉体;C:1.果胶酶活性,2.果胶酯酶抑制剂活性,3.钙离子结合,4.天冬酰胺酶活性,5.溶质质子逆向转运体活性,6.带状蛋白重链结合,7.1-磷脂酰肌醇结合,8.磷脂酰肌醇-4,5-二磷酸结合,9.钙调蛋白结合,10.磷脂酰肌醇-4,5-二磷酸结合,11.木聚糖转移酶活性,12.钙跨膜转运蛋白活性、磷酸化机制,13.SNARE结合,14.二聚体解离抑制剂活性,15.β-半乳糖苷酶活性,16.碳水化合物质子同向转运体活性,17.肌动蛋白纤维结合,18.质子输出型ATP酶活性、磷酸化机制,19.运输活动,20.氨基酸跨膜转运蛋白活性;D:1.果胶分解过程,2.细胞壁修饰,3.pH调节,4.单价无机阳离子转运,5.囊泡蛋白组装,6.茉莉酸介导的信号通路调控,7.花粉管生长,8.细胞膜囊泡形成,9.囊泡依赖性胞吞作用,10.细胞内信号转导,11.细胞尖端生长,12.胞吞作用,13.肽基-丝氨酸磷酸化,14.细胞发育性生长,15.多细胞生物生长过程,16.授粉过程,17.对创伤的反应,18.细胞内pH调节,19.防御反应调节,20.形态发育相关生长过程;E:1.质膜,2.内膜系统,3.细胞壁,4.细胞外区域,5.囊泡壳层,⒍ 被蛋白包裹的囊泡,⒎ 肌动蛋白细胞骨架,8.细胞膜组成部分,9.细胞顶端部分,10.顶端质膜,11.高尔基体,12.细胞边缘,13.花粉管,14.质膜区域,15.细胞质,16.膜,17.外部封装结构,18.细胞膜包裹的细胞突起,19.细胞突起,20.细胞连接点;F:1.果胶酶活性,2.果胶酯酶抑制剂活性,3.天冬酰胺酶活性,4.溶质质子逆向转运体活性,5.钙离子结合,6.带状蛋白重链结合,7.1-磷脂酰肌醇结合,8.磷脂酰肌醇-4,5-二磷酸结合,9.钙调蛋白结合,10.GTP酶激活剂活性,11.钙依赖型蛋白丝氨酸/苏氨酸激酶活性,12.二聚体解离抑制剂活性,13.SNARE结合,14.果胶裂解酶活性,15.碳水化合物质子同向转运体活性,16.肌动蛋白纤维结合,17.质子输出型ATP酶活性、磷酸化机制,18.跨膜受体蛋白丝氨酸/苏氨酸激酶活性,19.受体配体活性,20.受体调节活性。

Fig.4 GO enrichment analysis of differentially expressed genes A—C.BP,CC,MF of the FA vs SA treatment;D—F.BP,CC,MF of the FF vs SF treatment.A:1.Pectin catabolic process,2.Cell wall modification,3.Regulation of pH,4.Monovalent inorganic cation transport,5.Clathrin coat assembly,6.Pollen tube growth,7.Vesicle budding from membrane,8.Endocytosis,9.Clathrin-dependent endocytosis,10.Intracellular signal transduction,11.Xyloglucan metabolic process,12.Cell wall organization,13.Cell wall biogenesis,14.Carbohydrate transport,15.Rab protein signal transduction,16.Cell tip growth,17.Carbohydrate metabolic process,18.Developmental cell growth,19.Multi-multicellular organism process,20.Pollination;B:1.Plasma membrane,2.Extracellular region,3.Cell wall,4.Clathrin-coated vesicle,5.Clathrin-coated pit,6.Endomembrane system,7.Actin cytoskeleton,8.Appoplast,9.Uniplex complex,10.Apical part of cell,11.Apical plasma membrane,12.Pollen tube,13.Golgi apparatus,14.Plasma membrane region,15.Integral component of mitochondrial inner membrane,16.Cytoskeleton,17.Vacuolar proton-transporting V-type ATPase complex,18.Integral component of plasma membrane,19.Vacuole,20.Amyloplast;C:1.Pectinesterase activity,2.Pectinesterase inhibitor activity,3.Calcium ion binding,4.Aspartyl esterase activity,5.Solute proton antiporter activity,6.Clathrin heavy chain binding,7.1-phosphatidylinositol binding,8.Calmodulin binding,9.GTPase activator activity,10.Phosphatidylinositol-4,5-bisphosphate binding,11.Xyloglucan:xyloglucosyl transferase activity,12.Calcium transmembrane transporter activity,phosphorylative mechanism,13.SNARE binding,14.Rho GDP-dissociation inhibitor activity,15.Beta-galactosidase activity,16.Carbohydrate:proton symporter activity,17.Actin filament binding,18.Proton-exporting ATPase activity,phosphorylative mechanism,19.Uniporter activity,20.Amino acid transmembrane transporter activity;D:1.Pectin catabolic process,2.Cell wall modification,3.Regulation of pH,4.Monovalent inorganic cation transport,5.Callose coat assembly,6.Regulation of jasmonic acid mediated signaling pathway,7.Pollen tube growth,8.Vesicle budding from membrane,9.Clathrin-dependent endocytosis,10.Intracellular signal transduction,11.Cell tip growth,12.Endocytosis,13.Peptidyl-serine phosphorylation,14.Developmental cell growth,15.Multi-multicellular organism process,16.Pollination,17.Response to wounding,18.Regulation of intracellular pH,19.Regulation of defense response,20.Developmental growth involved in morphogenesis;E:1.Plasma membrane,2.Endomembrane system,3.Cell wall,4.Extracellular region,5.Clathrin-coated pit,6.Clathrin-coated vesicle,7.Actin cytoskeleton,8.Integral component of plasma membrane,9.Apical part of cell,10.Apical plasma membrane,11.Olgi apparatus,12.Cell periphery,13.Pollen tube,14.Plasma membrane region,15.Apoplast,16.Membrane,17.External encapsulating structure,18.Plasma membrane bounded cell projection,19.Cell projection,20.Cell junction;F:1.Pectinesterase activity,2.Pectinesterase inhibitor activity,3.Aspartyl esterase activity,4.Solute:proton antiporter activity,5.Calcium ion binding,6.Clathrin heavy chain binding,7.1-phosphatidylinositol binding,8.Phosphatidylinositol-4,5-bisphosphate binding,9.Calmodulin binding,10.GTPase activator activity,11.Calcium-dependent protein serine/threonine kinase activity,12.Rho GDP-dissociation inhibitor activity,13.SNARE binding,14.Pectate lyase activity,15.Carbohydrate:proton symporter activity,16.Actin filament binding,17.Proton-exporting ATPase activity,phosphorelay intermediate,18.Transmembrane receptor protein serine/threonine kinase activity,19.Receptor ligand activity,20.Receptor regulator activity.

图5 差异表达基因蛋白质互作网络图

Fig.5 Differential expression gene protein interaction network diagram

图6 差异表达基因的表达量热图(A)与qRT-PCR验证(B) 不同小写字母表示差异显著(P<0.05)。图7同。

Fig.6 Heat map of expression levels of differentially expressed genes(A)and qRT-PCR validation(B) Different lowercase letters indicate significant difference(P<0.05).The same as Fig.7.

图7 果胶裂解酶活性的测定

Fig.7 Determination of pectin lyase activity

参考文献 43

[1]	孙建. 豆类作物的营养分析与栽培技术[J]. 农业与技术, 2017, 37(3):41-42.doi:10.11974/nyyjs.20170229015.
	Sun J. Nutritional analysis and cultivation techniques of Leguminous crops[J]. Agriculture and Technology, 2017, 37(3):41-42.
[2]	孙妍妍, 赵丽梅, 张伟, 张春宝. 大豆杂种优势利用研究进展[J]. 大豆科技, 2021(6):26-35.doi:10.3969/j.issn.1674-3547.2021.06.006.
	Sun Y Y, Zhao L M, Zhang W, Zhang C B. Research progress on the utilization of soybean heterosis[J]. Soybean Science and Technology, 2021(6):26-35.
[3]	王茜, 陈景斌, 林云, 刘金洋, 薛晨晨, 闫强, 吴然然, 崔瑾, 陈新, 袁星星. 豆类作物雄性不育及杂种优势利用的研究进展[J]. 江苏农业科学, 2022, 50(4):9-16.doi:10.15889/j.issn.1002-1302.2022.04.002.
	Wang Q, Chen J B, Lin Y, Liu J Y, Xue C C, Yan Q, Wu R R, Cui J, Chen X, Yuan X X. Research progress on male sterility and utilization of hybrid vigor in Leguminous crops[J]. Jiangsu Agricultural Science, 2022, 50(4):9-16.
[4]	Nadeem M, Chen A D, Hong H L, Li D D, Li J J, Zhao D, Wang W, Wang X B, Qiu L J. GmMs1 encodes a kinesin-like protein essential for male fertility in soybean(Glycine max L.)[J]. Journal of Integrative Plant Biology, 2021, 63(6):1054-1064.doi:10.1111/jipb.13110. URL
[5]	Yang Y, Speth B D, Boonyoo N, Baumert E, Atkinson T R, Palmer R G, Sandhu D. Molecular mapping of three male-sterile,female-fertile mutants and generation of a comprehensive map of all known male sterility genes in soybean[J]. Genome, 2014, 57(3):155-160.doi:10.1139/gen-2014-0018. pmid: 24814801
[6]	Cervantes-Martinez I, Xu M, Zhang L, Huang Z, Kato K K, Horner H T, Palmer R G. Molecular mapping of male-sterility loci ms2 and ms9 in soybean[J]. Crop Science, 2007, 47(1):374-379.doi:10.2135/cropsci2006.03.0143. URL
[7]	Nie Z X, Zhao T J, Liu M F, Dai J Y, He T T, Lyu D, Zhao J M, Yang S P, Gai J Y. Molecular mapping of a novel male-sterile gene msNJ in soybean(Glycine max (L.)Merr.)[J]. Plant Reproduction, 2019, 32(4):371-380.doi:10.1007/s00497-019-00377-6.
[8]	Frasch R M, Weigand C, Perez P T, Palmer R G, Sandhu D. Molecular mapping of 2 environmentally sensitive male-sterile mutants in soybean[J]. The Journal of Heredity, 2011, 102(1):11-16.doi:10.1093/jhered/esq100. URL
[9]	Cervantes-Martinez I, Sandhu D, Xu M, Ortiz-Pérez E, Kato K K, Horner H T, Palmer R G. The male sterility locus ms3 is present in a fertility controlling gene cluster in soybean[J]. The Journal of Heredity, 2009, 100(5):565-570.doi:10.1093/jhered/esp054. URL
[10]	Ott A, Yang Y, Bhattacharyya M, Horner H T, Palmer R G, Sandhu D. Molecular mapping of D1,D2 and ms5 revealed linkage between the cotyledon color locus D2 and the male-sterile locus ms5 in soybean[J]. Plants, 2013, 2(3):441-454.doi:10.3390/plants2030441.
[11]	Albertsen M C, Palmer R G. A comparative light-and electron-microscopic study of microsporogenesis in male sterile(MS)and male fertile soybeans(Glycine max (L.)Merr.)[J]. American Journal of Botany, 1979, 66(3):253-265.doi:10.1002/j.1537-2197.1977.tb11916.x.
[12]	Delannay X, Palmer R G. Genetics and cytology of the ms4 male-sterile soybean[J]. Journal of Heredity, 1982, 73(3):219-223.doi:10.1093/oxfordjournals.jhered.a109621. URL
[13]	Graybosch R A, Palmer R G. Male sterility in soybean(Glycine max).i.phenotypic expression of the ms2 mutant[J]. American Journal of Botany, 1985, 72(11):1738-1750.doi:10.1002/j.1537-2197.1985.tb08447.x.
[14]	Palmer R G, Johns C W, Muir P S. Genetics and cytology of the ms3 male-sterile soybean[J]. Journal of Heredity, 1980, 71(5):343-348.doi:10.1093/oxfordjournals.jhered.a109383. URL
[15]	Skorupska H, Palmer R G. Genetics and cytology of the ms6 male-sterile soybean[J]. Journal of Heredity, 1989, 80(4):304-310.doi:10.1093/oxfordjournals.jhered.a110858. URL
[16]	Stelly D M, Palmer R G. A partially male-sterile mutant line of soybeans,Glycine max (L.) Merr:inheritance[J]. Euphytica, 1980, 29(2):295-303.doi:10.1007/BF00025126. URL
[17]	Palmer R G. Genetics of four male-sterile,female-fertile soybean mutants[J]. Crop Science, 2000, 40(1):78-83.doi:10.2135/cropsci2000.40178x. URL
[18]	Palmer R G, Gai J Y, Sun H A, Burton J W. Production and evaluation of hybrid soybean[J]. Plant Breading reviews, 2001, 21:263-307.doi:10.1002/9780470650196.CH7.
[19]	Kim D, Langmead B, Salzberg S L. HISAT:a fast spliced aligner with low memory requirements[J]. Nature Methods, 2015, 12(4):357-360.doi:10.1038/nmeth.3317.
[20]	Pertea M, Pertea G M, Antonescu C M, Chang T C, Mendell J T, Salzberg S L. StringTie enables improved reconstruction of a transcriptome from RNA-seq reads[J]. Nature Biotechnology, 2015, 33(3):290-295.doi:10.1038/nbt.3122. pmid: 25690850
[21]	Schmittgen T D, Livak K J. Analyzing real-time PCR data by the comparative CT method[J]. Nature Protocols, 2008, 3(6):1101-1108.doi:10.1038/nprot.2008.73. pmid: 18546601
[22]	贺庆梅. 柱花草突变体株系的育性鉴定[D]. 海口: 海南大学, 2012.
	He Q M. Fertility identification of the stylo mutant[D]. Haikou: Hainan University, 2012.
[23]	苏振新, 周鹊, 楼悦. 花药绒毡层发育及调控的分子研究进展[J]. 中国科学:生命科学, 2025, 55(2):206-225.
	Su Z X, Zhou Q, Lou Y. Advance in the anther tapetum development and their regulatory mechanisms[J]. Chinese Science:Life Sciences, 2025, 55(2):206-225.
[24]	Ito T, Nagata N, Yoshiba Y, Ohme-Takagi M, Ma H, Shinozaki K. Arabidopsis MALE STERILITY1 encodes a PHD-type transcription factor and regulates pollen and tapetum development[J]. The Plant Cell, 2007, 19(11):3549-3562.doi:10.1105/tpc.107.054536. URL
[25]	Xu J, Yang C Y, Yuan Z, Zhang D S, Gondwe M Y, Ding Z W, Liang W Q, Zhang D B, Wilson Z A. The ABORTED MICROSPORES regulatory network is required for postmeiotic male reproductive development in Arabidopsis thaliana[J]. The Plant Cell, 2012, 22(1):91-107.doi:10.1105/tpc.109.071803. URL
[26]	Yu J P, Zhao G L, Li W, Zhang Y, Wang P, Fu A G, Zhao L M, Zhang C B, Xu M. A single nucleotide polymorphism in an R2R3 MYB transcription factor gene triggers the male sterility in soybean ms6(Ames1)[J]. Theoretical and Applied Genetics, 2021, 134(11):3661-3674.doi:10.1007/s00122-021-03920-0.
[27]	雷霆. 兼职蛋白OsUGE1参与水稻花药绒毡层降解和育性形成的机理研究[D]. 重庆:西南大学, 2024.doi:10.27684/d.cnki.gxndx.2024.002309.
	Lei T. Mechanism of moonlighting protein OsUGE1on tapetum degradation and male fertility in rice (Oryza sativa L.)[D]. Chongqing: Southwest University,2024.
[28]	赵颖. 联合RNA-seq和iTRAQ技术挖掘大豆细胞质雄性不育相关基因[D]. 通辽: 内蒙古民族大学, 2023.doi:10.27228/d.cnki.gnmmu.2023.000405.
	Zhao Y. Combined RNA-seq and iTRAQ technology to mine genes related to cytoplasmic male sterility in soybean[D]. Tongliao: Inner Mongolia University for Nationalities,2023.
[29]	张敬敬, 李冰, 高秀瑞, 田鹏, 刘伟, 赵鑫泽, 刘会茹, 潘秀清, 武彦荣. 茄子反向温敏雄性不育系内源激素含量及相关基因表达分析[J]. 中国瓜菜, 2024, 37(11):27-37.doi:10.16861/j.cnki.zggc.2024.0449.
	Zhang J J, Li B, Gao X R, Tian P, Liu W, Zhao X Z, Liu H R, Pan X Q, Wu Y R. Analysis of endogenous hormones content and related gene expression in reverse therm-osensitive genic male sterile line of eggplant[J]. China Cucurbits and Vegetables, 2024, 37(11):27-37.
[30]	郭静文, 史晓蕾, 刘茜, 赵青松, 邸锐, 刘兵强, 闫龙, 王凤敏, 张孟臣, 赵宝华, 杨春燕. 基于转录组测序技术挖掘大豆蛋白质合成相关基因[J]. 华北农学报, 2019, 34(1):61-73.doi:10.7668/hbnxb.201751206.
	Guo J W, Shi X L, Liu Q, Zhao Q S, Di R, Liu B Q, Yan L, Wang F M, Zhang M C, Zhao B H, Yang C Y. Transcriptome analysis of protein synthesis related genes in soybean[J]. Acta Agriculturae Boreali-Sinica, 2019, 34(1):61-73. doi: 10.7668/hbnxb.201751206
[31]	林静, 林建新, 张扬, 卢和顶, 陈山虎, 廖长见. 利用转录组解析鲜食玉米闽甜6855的耐寒机制[J]. 华北农学报, 2022, 37(5):1-8.doi:10.7668/hbnxb.20193005.
	Lin J, Lin J X, Zhang Y, Lu H D, Chen S H, Liao C J. Transcriptomic analysis of the cold tolerance mechanism of fresh-eating corn Mintian 6855[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5):1-8. doi: 10.7668/hbnxb.20193005
[32]	张敬敬, 李冰, 史宇凡, 高秀瑞, 潘秀清, 宋雪, 武彦荣. 不同硬度西瓜果皮的转录组测序及相关基因表达分析[J]. 华北农学报, 2022, 37(3):44-52.doi:10.7668/hbnxb.20192957.
	Zhang J J, Li B, Shi Y F, Gao X R, Pan X Q, Song X, Wu Y R. Transcriptome sequencing and gene expression analysis of watermelon peel with different firmness[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3):44-52. doi: 10.7668/hbnxb.20192957
[33]	何德鑫. 大豆细胞质雄性不育系及其同型保持系的转录组分析[D]. 通辽:内蒙古民族大学, 2020.doi:10.27228/d.cnki.gnmmu.2020.000072.
	He D X. Transcriptome analysis of cytoplasmic male sterile lines and their maintainers in soybean[D]. Tongliao: Inner Mongolia University for Nationalities,2023.
[34]	张必周, 白晨, 吴新荣, 张自强, 王良, 韩平安, 张惠忠, 李晓东. 甜菜雄性不育系及保持系花蕾差异基因转录组测序分析[J]. 分子植物育种, 2024, 22(1):34-41.doi:10.13271/j.mpb.022.000034.
	Zhang B Z, Bai C, Wu X R, Zhang Z Q, Wang L, Han P A, Zhang H Z, Li X D. Transcriptome sequencing analysis of differentially genes between male sterile line and maintainer line in sugar beet[J]. Molecular Plant Breeding, 2024, 22(1):34-41.
[35]	范文强, 刘雪霞, 马小兰, 杨花, 朱金忠, 唐建宁, 岳思君, 郑蕊. 利用转录组数据挖掘枸杞雄性不育相关基因[J]. 中草药, 2023, 54(7):2226-2234.doi: 10.7501/j.issn.0253-2670.2023.07.023.
	Fan W Q, Liu X X, Ma X L, Yang H, Zhu J Z, Tang J N, Yue S J, Zheng R. Mining of male sterility-related genes in Lycium barbarum using transcriptome data[J]. Chinese Traditional and Herbal Drugs, 2023, 54(7):2226-2234.
[36]	Xin Q Q, Hu X Q, Zhang Y, Li D, Xu B H, Liu X M. Expression pattern analysis of key genes related to anther development in a mutant of male-sterile Betula platyphylla Suk[J]. Tree Genetics & Genomes, 2020, 16(2):33.doi:10.1007/s11295-020-1425-7.
[37]	Suzuki T, Narciso J O, Zeng W, van de Meene A, Yasutomi M, Takemura S, Lampugnani E R, Doblin M S, Bacic A, Ishiguro S. KNS4/UPEX1:a type Ⅱ Arabinogalactan β-(1,3)-galactosyltransferase required for pollen exine development[J]. Plant Physiology, 2017, 173(1):183-205.doi:10.1104/pp.16.01385. URL
[38]	Yin W Z, Yang H X, Wang Y T, Feng P, Deng Y, Zhang L S, He G H, Wang N. Oryza sativa PECTIN DEFECTIVE TAPETUM1 affects anther development through a pectin-mediated signaling pathway in rice[J]. Plant Physiology, 2022, 189(3):1570-1586.doi:10.1093/plphys/kiac172. URL
[39]	Zhao Q S, Tong Y, Yang C Y, Yang Y Q, Zhang M C. Identification and mapping of a new soybean male-sterile gene,mst-M[J]. Frontiers in Plant Science, 2019, 10:94.doi:10.3389/fpls.2019.00094. URL
[40]	Kim M J, Kim M, Lee M R, Park S K, Kim J. LATERAL organ boundaries DOMAIN(LBD) 10 interacts with SIDECAR POLLEN/LBD27 to control pollen development in Arabidopsis[J]. The Plant Journal, 2015, 81(5):794-809.doi:10.1111/tpj.12767. URL
[41]	Huang J, Wijeratne A J, Tang C, Zhang T Y, Fenelon R E, Owen H A, Zhao D Z. Ectopic expression of TAPETUM DETERMINANT1 affects ovule development in Arabidopsis[J]. Journal of Experimental Botany, 2016, 67(5):1311-1326.doi:10.1093/jxb/erv523. pmid: 26685185
[42]	Shi X, Sun X H, Zhang Z G, Feng D, Zhang Q, Han L D, Wu J X, Lu T G. GLUCAN SYNTHASE-LIKE 5(GSL5)plays an essential role in male fertility by regulating callose metabolism during microsporogenesis in rice[J]. Plant and Cell Physiology, 2015, 56(3):497-509.doi:10.1093/pcp/pcu193. URL
[43]	Wang D X, Skibbe D S, Walbot V. Maize Male sterile 8(Ms8),a putative β-1,3-galactosyltransferase,modulates cell division,expansion,and differentiation during early maize anther development[J]. Plant Reproduction, 2013, 26(4):329-338.doi:10.1007/s00497-013-0230-y. URL

选择文件类型/文献管理软件名称

选择包含的内容

RNA-Seq技术鉴定大豆雄性不育候选基因

Identification of Candidate Genes for Male Sterile in Soybean by RNA-Seq

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 43

相关文章 15

编辑推荐

Metrics

本文评价

[1]	柯丹霞, 周兆源, 侯仕博, 张可馨, 宋晓莉, 林佳诺. *大豆蛋白磷酸酶基因GmPP2C72的耐盐功能分析*[J]. 华北农学报, 2026, 41(2): 12-20.
[2]	张向前, 王鹏举, 胡子全, 尚云秋, 赵竹, 陈欢, 杜世州, 乔玉强. 大豆生长、光合及产量品质对叶面肥施用次数和时期的响应[J]. 华北农学报, 2026, 41(1): 79-85.
[3]	刘丽, 张微, 徐洪岩, 张明爽, 王若丁, 钱春荣. 蚯蚓粪和化肥不同比例配施对大豆产量和品质的影响[J]. 华北农学报, 2025, 40(S1): 190-196.
[4]	赵志鑫, 刘宁宁, 徐海风, 王亚琪, 傅蒙蒙, 李曙光, 余希文. 模拟涝害胁迫下大豆生理和转录组分析[J]. 华北农学报, 2025, 40(S1): 9-19.
[5]	曹金龙, 王莉, 曹玲芳, 郝凯茵, 顾继靓, 王宇, 车志军. 大豆OFP基因家族鉴定及其响应干旱胁迫和盐胁迫的表达分析[J]. 华北农学报, 2025, 40(6): 1-10.
[6]	陈玥含, 魏玉, 冯燕, 赵丽, 闫龙, 杨庆, 刘智. 低温胁迫条件下大豆萌发期转录组分析[J]. 华北农学报, 2025, 40(6): 11-20.
[7]	栾崇圣, 胡蕾蕾, 吴旗, 林雷利, 汪小宇, 李笑笑, 车钊, 王晓波, 宋贺, 吴巩. 增温和有机无机肥配施对大豆光合特性和产量的影响[J]. 华北农学报, 2025, 40(6): 139-151.
[8]	林静, 史晓蕾, 徐俊杰, 于翠红, 曹志敏, 唐晓东, 杨春燕, 张孟臣, 闫龙. *抗大豆花叶病毒基因位点qTsmv-3候选基因克隆及功能分析*[J]. 华北农学报, 2025, 40(4): 57-64.
[9]	桑莹莹, 李珊珊, 鲍薇, 徐东, 张雪, 赵艳. 大豆P34蛋白基因启动子克隆及其调控活性分析[J]. 华北农学报, 2025, 40(1): 22-28.
[10]	黄友举, 于泳波, 逄翠晶, 孙轼絮, 路晨, 于延冲. *大豆GmWRKY44生物信息学分析及功能验证*[J]. 华北农学报, 2025, 40(1): 29-35.
[11]	赵圆, 刘智, 杨庆, 王宇, 王艳丽, 闫龙, 张锴, 史晓蕾, 刘晓燕. 大豆-根瘤菌共生结瘤能力鉴定培养条件的筛选[J]. 华北农学报, 2024, 39(S1): 125-134.
[12]	邢宝瑞, 赵海平, 李光玉, 马泽芳, 孙红梅, 胡肖, 谭展清, 刘振. PTH 1-34对鹿茸间充质干细胞成骨机制的转录组学分析[J]. 华北农学报, 2024, 39(S1): 344-352.
[13]	张向前, 杜世州, 乔玉强, 曹承富, 李玮, 赵竹, 陈欢, 丁永刚, 尚云秋. 大豆生长及产量和品质对不同施肥模式的响应[J]. 华北农学报, 2024, 39(6): 186-192.
[14]	徐天驰, 陈松鹤, 王存虎, 钟永嘉. 高低结瘤对大豆根际相关微生物群落的调控作用[J]. 华北农学报, 2024, 39(6): 193-201.
[15]	樊超, 毕影东, 李炜, 梁文卫, 刘淼, 刘建新, 杨光, 邸树峰. 基于BSA-seq的大豆棕色荚皮L2基因定位[J]. 华北农学报, 2024, 39(4): 64-71.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

RNA-Seq技术鉴定大豆雄性不育候选基因

Identification of Candidate Genes for Male Sterile in Soybean by RNA-Seq

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 43

相关文章 15

编辑推荐

Metrics

本文评价