类猪圆环病毒P1的感染及其分子特征

doi:10.7668/hbnxb.2013.05.006

华北农学报 ›› 2013, Vol. 28 ›› Issue (5): 34-37. doi: 10.7668/hbnxb.2013.05.006

所属专题： 畜牧；

类猪圆环病毒P1的感染及其分子特征

温立斌¹, 何孔旺¹, 王凤芝², 俞正玉¹, 茅爱华¹, 解建平¹

1 江苏省农业科学院兽医研究所农业部兽用生物制品工程技术重点实验室国家兽用生物制品工程技术研究中心江苏南京 210014;
2 南京农业大学江苏南京 210095

收稿日期:2013-07-07 出版日期:2013-10-28
作者简介:温立斌(1967－),男,河北宣化人,研究员,博士,主要从事动物分子病毒学与免疫学研究。
基金资助:
国家自然科学基金项目(31272574;30972184);江苏省农业科技自主创新资金项目(CX(11)2060)

Porcine Circovirus like Virus P1 Infection and Its Molecular Characteristics

WEN Li-bin¹, HE Kong-wang¹, WANG Feng-zhi², YU Zheng-yu¹, MAO Ai-hua¹, XIE Jian-ping¹

1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China;
2 Nanjing Agricultural University, Nanjing 210095, China

Received:2013-07-07 Published:2013-10-28

摘要/Abstract

摘要： 类猪圆环病毒P1是新发现的一种病原,能引起仔猪出现类似猪断奶后多系统衰竭综合征症状,是目前所知的具有最小基因组的病毒。为了解P1感染范围和基因组分子特征,采用PCR方法扩增P1全基因组,克隆测序后,应用生物信息学进行序列同源性比较及遗传进化分析。结果显示,P1已在我国猪场流行,分离株之间的核苷酸序列同源性为99.1%～100%,可分为2个亚分支;同时免疫组化结果也证实了P1在样品中的分布。

关键词: 类猪圆环病毒P1, PCR, 免疫组化, 分子特征

Abstract: Porcine circovirus like virus P1 is a new pathogen emerging in porcine and causing postweaning mul-tisystemic wasting syndrome ( PMWS)-like signs.P1 has the smallest genome up to now. To investigate the preva-lence and molecular characteristics of P1, complete viral DNA genome sequences were amplified by PCR, cloned and sequenced. Bioinformatics was employed to analyze the nucleotide sequence and phylogeny. The results of this study indicated that the prevalence of P1 in the pig farms were quite high,the nucleotide sequence similarity of P1 was 99. 1%to 100%,and it could be classified in two sublineage;Meanwhile,distribution of P1 in samples were al-so detected by immunohistochemistry.

Key words: Porcine circovirus like virus P1, PCR, Immunohistochemistry, Molecular characteristics

中图分类号:

S852.65

温立斌, 何孔旺, 王凤芝, 俞正玉, 茅爱华, 解建平. 类猪圆环病毒P1的感染及其分子特征[J]. 华北农学报, 2013, 28(5): 34-37. doi: 10.7668/hbnxb.2013.05.006.

WEN Li-bin, HE Kong-wang, WANG Feng-zhi, YU Zheng-yu, MAO Ai-hua, XIE Jian-ping. Porcine Circovirus like Virus P1 Infection and Its Molecular Characteristics[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(5): 34-37. doi: 10.7668/hbnxb.2013.05.006.

http://www.hbnxb.net/CN/Y2013/V28/I5/34

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