摘要： 为了对转1Dx5基因小麦的T₁进行遗传分析，将优质高分子量麦谷蛋白亚基（HMW-GS）基因1Dx5转入新疆耐盐小麦品种新冬26，在蛋白质水平上经SDS-PAGE检测，有37粒小麦种子1Dx5基因表达。经过继代培育，得到37个T₁转基因株系，SDS-PAGE分析T₁转基因小麦籽粒的HMW-GS组成，结果表明：在蛋白质水平，HMW-GS的组成在T₁小麦种子中有3种表型：表型Ⅰ，外源1Dx5基因没有表达；表型Ⅱ，外源1Dx5基因表达；表型Ⅲ，内源1Dx2基因没有表达。这3种表型出现的概率分别约为34.3%，45.9%和19.8%。该研究成功获得了新的HMW-GS组成的小麦，为选育优质小麦提供分子依据。

关键词: 小麦, 优质, SDS-PAGE, HMW-GS

Abstract: In order to carry out genetic analysis of T₁ generation of transgenic wheat with 1Dx5 gene,the Xinjiang winter common wheat Xindong No.26 were transformed with high-molecular-weight glutenin subunits (HMW-GS) gene 1Dx5,detected by SDS-PAGE,was found expression of 1Dx5 gene in 37 wheat seeds at the protein level.After subculture cultivation,37 T₁ generation transgenic lines were obtained.SDS-PAGE analysis of T₁ generation transgenic wheat seeds,the results showed that the composition of HMW-GS were found three phenotypes at protein level,phenotypic Ⅰ,the foreign 1Dx5 gene did not express;phenotypic Ⅱ,the foreign 1Dx5 gene express;phenotypic Ⅲ,endogenous 1Dx2 gene did not express.The probability of these three phenotypes were approximately 34.3%,45.9% and 19.8%,respectively.The study successfully obtained new types of HMW-GS composition,providing molecular evidence for the selection of high quality wheat.

Key words: Wheat, High quality, SDS-PAGE, HMW-GS

中图分类号: 

• Q78