华北农学报 ›› 2016, Vol. 31 ›› Issue (S1): 150-155. doi: 10.7668/hbnxb.2016.S1.027

• 作物遗传育种·种质资源·生物技术 • 上一篇    下一篇

苜蓿属4种不同类型材料亲缘关系研究

刘磊, 李志勇, 师文贵, 李鸿雁, 赵金梅, 黄帆, 李俊   

  1. 中国农业科学院 草原研究所, 农业部草原资源与利用重点开放实验室, 内蒙古 呼和浩特 010010
  • 收稿日期:2016-05-05 出版日期:2016-12-28
  • 通讯作者: 李志勇(1965-),男,内蒙古乌兰察布人,研究员,博士,硕士生导师,主要从事牧草种质资源研究。
  • 作者简介:刘磊(1981-),男,内蒙古乌兰察布人,助理研究员,博士,主要从事牧草种质资源的分类及育种研究。
  • 基金资助:
    国家自然科学基金项目(31402122;31302018);农业部牧草种质资源保护项目(2014-08);科技部国家科技基础条件平台课题(NICGR2015-065)

The Study of Genetic Relationship of Four Different Materials in Medicago L.

LIU Lei, LI Zhiyong, SHI Wengui, LI Hongyan, ZHAO Jinmei, HUANG Fan, LI Jun   

  1. Grassland Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Grassland Resources and Utilization, Huhhot 010010, China
  • Received:2016-05-05 Published:2016-12-28

摘要: 利用表型性状和ISSR分子标记技术对3份白花苜蓿、3份黄花苜蓿、5份紫花苜蓿和3份扁蓿豆共计14份苜蓿属材料进行研究,分析其遗传关系,进一步解析其之间的分类学关系。从表型性状来看,白花苜蓿区别于其他苜蓿的主要形态学特征是花色为乳白色,荚果1.0~1.5个螺旋,从聚类分析来看,3份扁蓿豆材料和11份苜蓿材料非常明显的聚成了2类,说明扁蓿豆与苜蓿还是有较大的区别,在遗传距离15的地方,白花苜蓿、紫花苜蓿和黄花苜蓿各单独聚为1类;从亲缘关系来看,扁蓿豆与黄花苜蓿的遗传距离最近,与紫花苜蓿的最远,白花苜蓿次之,相对于紫花苜蓿,白花苜蓿与黄花苜蓿的遗传距离较近;从ISSR分子标记水平来看,4类苜蓿种质资源在遗传距离0.80的地方分别聚为单独的4类,从遗传关系来看,白花苜蓿相对于黄花苜蓿与紫花苜蓿的遗传关系更近,但紫花苜蓿与白花苜蓿和黄花苜蓿的遗传关系几乎相等。

关键词: 苜蓿, 表型性状, ISSR分子标记, 亲缘关系

Abstract: Morphology and ISSR Marker and their clusters analysis were carried out in 5 M. sativa L.,3 M. falcata L.,3 alfalfa with cream flower and 3 M. ruthenica L. for analysing the genetic relationship to further reveal the taxonomic relationship. The results showed alfalfa with cream flower had the significant differences in flower color(cream) and pod(1.0-1.5 coils). In cluster analysis,2 groups were clustered between 3 M. ruthenica L. and 11 Medicago L. which meant the significant at the genetic distance of 15;at the genetic distance of 10,3 groups were clustered in 5 M. sativa L.,3 M. falcate L. and 3 alfalfa with cream flower. From the relationship, M. ruthenica L. had the closer relationship than that of M. sativa L. and alfalfa with cream flower;alfalfa with cream flower had the closer relationship than that of M. sativa L.. At the genetic distance of 0.80,4 groups were clustered in 5 M. sativa L.,3 M. falcata L.,3 alfalfa with cream flower and M. ruthenica L. in ISSR Marker,by the genetic relationship,alfalfa with cream flower had the closer genetic relationship with M. sativa L. comparing with M. falcate L.,but, M. sativa L. had the equal genetic relationship with alfalfa with cream flower and with M. falcate L..

Key words: Medicago L., Morphology, ISSR Marker, Genetic relationship

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引用本文

刘磊, 李志勇, 师文贵, 李鸿雁, 赵金梅, 黄帆, 李俊. 苜蓿属4种不同类型材料亲缘关系研究[J]. 华北农学报, 2016, 31(S1): 150-155. doi: 10.7668/hbnxb.2016.S1.027.

LIU Lei, LI Zhiyong, SHI Wengui, LI Hongyan, ZHAO Jinmei, HUANG Fan, LI Jun. The Study of Genetic Relationship of Four Different Materials in Medicago L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 150-155. doi: 10.7668/hbnxb.2016.S1.027.

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