华北农学报 ›› 2015, Vol. 30 ›› Issue (1): 158-161. doi: 10.7668/hbnxb.2015.01.026

所属专题: 甜椒辣椒

• 论文 • 上一篇    下一篇

辣椒C.annuum×C.chinense种间杂种的获得及鉴定

孙润生, 王述彬, 刁卫平, 刘金兵, 潘宝贵, 戈伟, 郭广君   

  1. 江苏省农业科学院 蔬菜研究所, 江苏 南京 210014
  • 收稿日期:2014-11-28 出版日期:2015-02-28
  • 通讯作者: 王述彬(1963-),男,江苏姜堰人,研究员,博士,主要从事辣椒遗传育种研究。
  • 作者简介:孙润生(1987-),女,黑龙江大庆人,在读硕士,主要从事辣椒遗传育种研究。
  • 基金资助:
    国家高技术研究发展计划"863"计划项目(2012AA100103);现代农业产业技术体系建设专项基金项目(CARS-25);江苏省农业科技自主创新基金项目[CX(12)1004]

Interspecific Hybridization and Identification of Hybrid Between C.annuum and C.chinense

SUN Run-sheng, WANG Shu-bin, DIAO Wei-ping, LIU Jin-bing, PAN Bao-gui, GE Wei, GUO Guang-jun   

  1. Institute of Vegetable Crops, Jiangsu Academy of Agricultural Science, Nanjing 210014, China
  • Received:2014-11-28 Published:2015-02-28

摘要: 种间杂交是种质创新和利用外源优异基因的重要途径。运用有性杂交方法,以浓紫色辣椒 C.annuum PBC1366为母本(P1)、强辣 C.chinense PI439487为父本(P2)进行种间杂交,获得了种间杂种植株。对杂种F1的19个表型性状进行了观察比较,结果表明,F1在生长势方面具有明显的杂种优势,其他表型性状介于父母本之间。种间F1花粉可染率为64.4%,形态学观察和EST-SSR标记分析结果明确了种间杂种F1的真实性。C.annuum × C.chinense 种间杂种的获得为辣椒种间遗传图谱构建、辣椒香味和花青素代谢途径相关基因的定位奠定了基础。

关键词: 辣椒, 种间杂交, 表型性状, SSR

Abstract: Interspecific hybridization is the important way of excellent germplasm innovation and utilization of exogenous gene.With manual pollination, an interspecific hybrid was obtained from the cross between thick purple pepper C.annuum PBC1366 as female parent (P1) and strong spicy pepper C.chinense PI439487 as male parent.19 phenotypic traits were observed and compared among P1, F1 and P2.The results showed that the F1 had heterosis significantly and majority of the other phenotypic traits were intermediate to that of either parent.The pollen stainability percentages of interspecific F1 was 64.4%, the nature of hybrid was confirmed by morphological observation and EST-SSR marker analysis.The obtainment of the interspecific hybrid of C.annuum and C.chinense afford a basis for genetic map construction, cocalization the fruit flavor gene, and anthocyanins metabolic pathway gene in pepper.

Key words: Pepper, Interspecific hybridization, Phenotypic traits, SSR

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引用本文

孙润生, 王述彬, 刁卫平, 刘金兵, 潘宝贵, 戈伟, 郭广君. 辣椒C.annuum×C.chinense种间杂种的获得及鉴定[J]. 华北农学报, 2015, 30(1): 158-161. doi: 10.7668/hbnxb.2015.01.026.

SUN Run-sheng, WANG Shu-bin, DIAO Wei-ping, LIU Jin-bing, PAN Bao-gui, GE Wei, GUO Guang-jun. Interspecific Hybridization and Identification of Hybrid Between C.annuum and C.chinense[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 158-161. doi: 10.7668/hbnxb.2015.01.026.

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