辣椒雄性不育系和保持系<i>CaMADS6</i>基因克隆及表达分析

doi:10.7668/hbnxb.20193440

摘要/Abstract

摘要：

为探究CaMADS6表达量与辣椒花器官发育之间的关系,以辣椒不育系9704A和保持系9704B为试验材料,克隆CaMADS6进行生物信息学预测,并分析该基因在两系中的时空表达特性。结果表明,从9704A和9704B中克隆得到的CaMADS6编码区序列一致,序列长744 bp,编码247个氨基酸残基。CaMADS6蛋白相对分子量为28.67 ku,理论等电点为8.98,为亲水性蛋白,无跨膜结构,二级结构中α螺旋占57.49%、延伸链占8.91%、无规则卷曲占28.74%。CaMADS6与辣椒CaFUL2同源性100%,蛋白结构域具有典型的MADS-box特征;CaMADS6在两系各发育时期不同器官中均有表达,表达量大小均为花蕾中最高、其次为叶和茎,根中几乎不表达。9704A茎中CaMADS6表达量在苗期、花蕾期和成株期3个发育时期中均显著低于9704B茎中的表达量,叶中CaMADS6表达量在成株期极显著低于9704B叶中表达量。花蕾中CaMADS6表达量在花蕾期和成株期均极显著高于9704B花蕾中表达量,分别为9704B的2.2,3.5倍;CaMADS6在两系植株花器官不同部位均能表达,表达量由大到小依次为花萼、花冠、子房、花药,其中9704A花萼和花冠中CaMADS6表达量极显著或显著低于9704B,分别为9704B花萼、花冠中CaMADS6表达量的66%,83%,花药中CaMADS6表达量为9704B表达量的34倍,两者差异极显著,推测CaMADS6基因在花药中的异常表达与辣椒雄性不育密切相关。

关键词: 辣椒, 雄性不育, CaMADS6, 基因克隆, 表达分析

Abstract:

To explore the relationship level between CaMADS6 expression and floral organ development of pepper,CaMADS6 was cloned for bioinformatics prediction from the sterile line 9704A and the maintainer line 9704B,and the spatiotemporal expression characteristics of this gene were analyzed.The results showed that the CaMADS6 cloned from 9704A and 9704B had the same coding sequence,with a full length of 744 bp and encoding 247 amino acid residues.CaMADS6 protein had the relative molecular weight of 28.67 ku and theoretical isoelectric point of 8.98.It was a hydrophilic protein with no transmembrane structure.The secondary structure of CaMADS6 consisted of 57.49% α-helices,8.91% extended chains and 28.74% irregular coils.The CaMADS6 protein shared 100% homology with CaFUL2,which possessed a typical MADS-box characteristics.CaMADS6 was expressed in different organs of 9704A and 9704B at different developmental stages and the expression was the highest in flower buds,followed by leaves and stems,and almost no expression in roots.The expression of CaMADS6 in stems of 9704A was significantly lower than that of 9704B at seedling,flower bud and adult stages,and the expression of CaMADS6 in leaves was significantly lower than that of 9704B at the adult stage.The expression level of CaMADS6 in flower buds of 9704A was extremely significantly higher than that of 9704B at flower bud and adult stage,and its expression level was 2.2 and 3.5 times higher than in 9704B,respectively;CaMADS6 gene was expressed in different parts of flower organs of 9704A and 9704B,and the expression of CaMADS6 gene from high to low was calyx,corolla,ovary and anther.The expression level of CaMADS6 in the calyx and corolla of 9704A was extremely significantly or significantly lower than that of 9704B,the expression level of CaMADS6 were 66%,83% of 9704B,respectively.The expression level of CaMADS6 in 9704A anther was 34 times higher than in 9704B,and the difference between the two groups was extremely significant,predicting the abnormal expression of CaMADS6 in anthers was closely related to pepper male sterility.

Key words: Pepper, Male sterility, CaMADS6, Gene cloning, Expression analysis

廖铭宇, 肖佳林, 李丽缘, 宋钰, 黄湖荣, 杨博智. 辣椒雄性不育系和保持系CaMADS6基因克隆及表达分析[J]. 华北农学报, 2023, 38(1): 46-52. doi: 10.7668/hbnxb.20193440.

LIAO Mingyu, XIAO Jialin, LI Liyuan, SONG Yu, HUANG Hurong, YANG Bozhi. Cloning and Expression Analysis of CaMADS6 in Cytoplasmic Male Sterile Line and Maintainer Line of Pepper[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(1): 46-52. doi: 10.7668/hbnxb.20193440.

http://www.hbnxb.net/CN/Y2023/V38/I1/46

图/表 7

图1 CaMADS6基因PCR扩增条带

Fig.1 The amplification bank of CaMADS6 gene M.5 kb DNA ladder。

表1 CaMADS6氨基酸组成及比例

Tab.1 The amino acid composition and proportion of CaMADS6

氨基酸组成 Amino acid composition	氨基酸数目 Number of amino acids	所占比例/% Ration	氨基酸组成 Amino acid composition	氨基酸数目 Number of amino acids	所占比例/% Ration	氨基酸组成 Amino acid composition	氨基酸数目 Number of amino acids	所占比例/% Ration
Ala	13	5.3	Arg	19	7.7	Asn	17	6.9
Asp	9	3.6	Cys	2	0.8	Gln	22	8.9
Glu	23	9.3	Gly	13	5.3	His	11	4.5
Ile	7	2.8	Leu	28	11.3	Lys	17	6.9
Met	7	2.8	Phe	5	2.0	Pro	7	2.8
Ser	21	8.5	Thr	7	2.8	Trp	3	1.2
Tyr	6	2.4	Val	10	4.0

图2 CaMADS6基因编码区及对应的氨基酸序列

Fig.2 The coding region and its corresponding amino acid sequences of CaMADS6

图3 辣椒CaMADS6与其他物种FUL2氨基酸序列比对红色线段表示MADS_MEF2_like结构域,由78个氨基酸组成(2—79);黑色线段表示K-box结构域,由84个氨基酸组成(90—173);蓝色阴影表示不同的氨基酸残基;黑色阴影表示相同的氨基酸残基。CaMADS6.辣椒(AF130118.2);CaFUL2.辣椒(NM_001324623.2);SlFUL2.番茄(NM_001307938.1);BsFUL2.大花曼陀罗(MT069983.1);PpFUL2.毛酸浆(KC414862.1);NtFUL2.烟草(NM_001325205.1);WsFUL2.睡茄(MH931169.1);SjFUL2.扭管花(MH931166.1);CnFUL2.夜香木(MT069971.1);PeFUL2.长蕊矮牵牛(MH931145.1)。

Fig.3 Alignment of amino acid sequences between CaMADS6 of pepper and FUL2 of other species The red and black line segments indicate MADS_MEF2_like structural domain consisting of 78 amino acids(residues 2—79)and the K-box structural domainconsisting of 84 amino acids(residues 90—173),respectively;the blue shading indicates different amino acid residues and black shading indicates identical amino acid residues.CaMADS6.Capsicum annuum (AF130118.2);CaFUL2.Capsicum annuum (NM_001324623.2);SlFUL2.Solanum lycopersicum (NM_001307938.1);BsFUL2.Brugmansia suaveolens (MT069983.1);PpFUL2.Physalis pubescens (KC414862.1);NtFUL2.Nicotiana tabacum (NM_001325205.1);WsFUL2.Withania somnifera (MH931169.1);SjFUL2.Streptosolen jamesonii (MH931166.1);CnFUL2.Cestrum nocturnum (MT069971.1);PeFUL2.Petunia exserta (MH931145.1).

图4 辣椒CaMADS6与其他物种FUL氨基酸序列进化树分析 TwFUL-L.雷公藤(NC_052235.1);PvFUL-L.开心果(NC_048444.1);NtFUL-L.烟草(NW_008885358.1);CiFUL-L.核桃(NC_056754.1);QlFUL-L.栎树(NC_044905.1);RcFUL-L.月季(NC_037089.1);VrFUL-L.河岸葡萄(NC_048444.1);VvFUL-L.酿酒葡萄(NC_012020.3);GhFUL-L.棉花(NC_053443.1);MeFUL-L.木薯(NC_035161.2);EgFUL-L.大桉树(NC_052612.1);SsFUL-L.一串红(NC_056042.1);NtFUL1.烟草(NW_015899026.1);SlFUL1.番茄(NC_015443.3);StFUL1.马铃薯(NW_006239107.1);InFUL1.日本牵牛花(NW_017618323.1);SoFUL.菠菜(NW_018932157.1);BnFUL.油菜(NW_019168750.1);AtFUL.拟南芥(NC_003076.8);BoFUL-a.野生卷心菜(NC_027749.1);BoFUL-b.野生卷心菜(NC_027754.1);BoFUL-c/BoFUL-d.野生卷心菜(NC_027756.1)。

Fig.4 Phylogenetic tree analysis of amino acid sequences of CaMADS6 in pepper and FUL in other species TwFUL-L.Tripterygium wilfordii (NC_052235.1);PvFUL-L.Pistacia vera (NC_048444.1);NtFUL-L.Nicotiana tomentosiformis(NW_008885358.1);CiFUL-L.Carya illinoinensis (NC_056754.1);QlFUL-L.Quercus lobata(NC_044905.1);RcFUL-L.Rosa chinensis(NC_037089.1);VrFUL-L.Vitis riparia(Riverbank grape)(NC_048444.1);VvFUL-L.Vitis riparia (Wine grapes)(NC_012020.3);GhFUL-L.Gossypium hirsutum(Cotton)(NC_053443.1);MeFUL-L.Manihot esculenta(Cassava)(NC_035161.2);EgFUL-L.Eucalyptus grandis(NC_052612.1);SsFUL-L.Salvia splendens(NC_056042.1);NtFUL1.Nicotiana tomentosiformis(NW_015899026.1);SlFUL1.Nicotiana tabacum(Common tobacco)(NC_015443.3);StFUL1.Solanum tuberosum(Potato)(NW_006239107.1);InFUL1.Ipomoea nil (Japanese morning glory)(NW_017618323.1);SoFUL.Spinaciaoleracea(Spinach)(NW_018932157.1);BnFUL.Brassica napus(Rape)(NW_019168750.1);AtFUL.Arabidopsis thaliana(Thale cress)(NC_003076.8);BoFUL-a.Brassica oleracea(Wild cabbage)(NC_027749.1);BoFUL-b.Brassica oleracea(Wild cabbage)(NC_027754.1);BoFUL-c/BoFUL-d.Brassica oleracea(Wild cabbage)(NC_027756.1).

图5 CaMADS6基因在9704A与9704B不同时期不同器官中的相对表达量以CaMADS6基因在9704A苗期茎中的表达量为对照进行方差分析。不同小写字母表示P<0.05,不同大写字母表示P<0.01。图6同。

Fig.5 The relative expression of CaMADS6 in the different organs at different stages of 9704A and 9704B The expression of CaMADS6 gene in stems of 9704A seedling was used as the control for analysis of variance.The different lowercase letters indicated significant difference P<0.05 and the different capital letters indicated extremely significant difference P<0.01.The same as Fig.6.

图6 CaMADS6基因在9704A与9704B花器官不同部位中的相对表达量以CaMADS6基因在9704A花药中表达量为对照进行方差分析。

Fig.6 The relative expression of CaMADS6 gene in different parts of floral organs of 9704A and 9704B The expression of CaMADS6 gene in anthers of 9704A was used as the control for analysis of variance.

参考文献 21

[1]	邹学校, 朱凡. 辣椒传入中国的途径与传播路径[J]. 湖南农业大学学报(自然科学版), 2020, 46(6):629-640.doi:10.13331/j.cnki.jhau.2020.06.001. doi: 10.13331/j.cnki.jhau.2020.06.001
	Zou X X, Zhu F. The path of pepper introduction into China and its spreading route in China[J]. Journal of Hunan Agricultural University (Natural Sciences), 2020, 46(6):629-640.
[2]	王姣, 张水, 张婧柔, 邵贵芳, 邓明华. 辣椒胞质雄性不育系CaCOX3的克隆与表达分析[J]. 生物技术通报, 2019, 35(4):1-6.doi:10.13560/j.cnki.biotech.bull.1985.2018-0984. doi: 10.13560/j.cnki.biotech.bull.1985.2018-0984
	Wang J, Zhang S, Zhang J R, Shao G F, Deng M H. Cloning and expression analysis of CaCOX3 gene from pepper cytoplasmic male sterile line[J]. Biotechnology Bulletin, 2019, 35(4):1-6.
[3]	邓明华, 邹学校, 霍金龙, 文锦芬, 朱海山, 张竹青, 赵凯. 辣椒核质互作雄性不育系线粒体不育基因CoxⅡ和atp6的克隆与序列分析[J]. 西北植物学报, 2010, 30(10):1935-1940.
	Deng M H, Zou X X, Huo J L, Wen J F, Zhu H S, Zhang Z Q, Zhao K. Clone and sequence analysis of the specific mitochondrial genes CoxⅡ and atp6 related to cytoplasmic male sterile in pepper[J]. Acta Botanica Boreali-Occidentalia Sinica, 2010, 30(10):1935-1940.
[4]	吕俊恒, 文锦芬, 莫云容, 赵凯, 马仲飞, 邓明华. 辣椒雄性不育系葡萄糖-6-磷酸脱氢酶基因的克隆及表达分析[J]. 西北植物学报, 2015, 35(4):682-687.doi:10.7606/j.issn.1000-4025.2015.04.0682. doi: 10.7606/j.issn.1000-4025.2015.04.0682
	Lü J H, Wen J F, Mo Y R, Zhao K, Ma Z F, Deng M H. Clone and expression analyses of CaG6PDH gene from pepper cytoplasmic male sterility line[J]. Acta Botanica Boreali-Occidentalia Sinica, 2015, 35(4):682-687.
[5]	张婧柔, 邵贵芳, 王姣, 张水, 杨婷玉, 邓明华. 辣椒胞质雄性不育系线粒体基因CaATP9的克隆与表达[J]. 生物技术通报, 2019, 35(11):9-15.doi:10.13560/j.cnki.biotech.bull.1985.2019-0283. doi: 10.13560/j.cnki.biotech.bull.1985.2019-0283
	Zhang J R, Shao G F, Wang J, Zhang S, Yang T Y, Deng M H. Clone and expression profile analyses of the gene CaATP9 in pepper(Capsicum annuum L.)male sterility line[J]. Biotechnology Bulletin, 2019, 35(11):9-15.
[6]	王莹, 穆艳霞, 王锦. MADS-box基因家族调控植物花器官发育研究进展[J]. 浙江农业学报, 2021, 33(6):1149-1158.doi:10.3969/j.issn.1004-1524.2021.06.21. doi: 10.3969/j.issn.1004-1524.2021.06.21
	Wang Y, Mu Y X, Wang J. Research progress of floral development regulation by MADS-box gene family[J]. Acta Agriculturae Zhejiangensis, 2021, 33(6):1149-1158.
[7]	吴雨. 影响水稻花发育的MADS-box基因研究进展[J]. 科学技术创新, 2018(8):154-155.doi:10.3969/j.issn.1673-1328.2018.08.094. doi: 10.3969/j.issn.1673-1328.2018.08.094
	Wu Y. Research progress of MADS-box gene affecting rice flower development[J]. Scientific and Technological Innovation, 2018(8):154-155.
[8]	焦志鑫, 李俊畅, 牛吉山. 小麦MIKC型MADS-box基因家族分析[J]. 农业生物技术学报, 2017, 25(11):1756-1769.doi:10.3969/j.issn.1674-7968.2017.11.003. doi: 10.3969/j.issn.1674-7968.2017.11.003
	Jiao Z X, Li J C, Niu J S. Analysis of MIKC-type MADS-box gene family in wheat(Triticum aestivum)[J]. Journal of Agricultural Biotechnology, 2017, 25(11):1756-1769.
[9]	杨贞妮. 番茄花器官发育相关转录因子的研究进展[J]. 安徽农学通报, 2017, 23(11):27-32,51.doi:10.16377/j.cnki.issn1007-7731.2017.11.009. doi: 10.16377/j.cnki.issn1007-7731.2017.11.009
	Yang Z N. The recent advances in the development of transcription factors involved in floral organ development in tomato[J]. Anhui Agricultural Science Bulletin, 2017, 23(11):27-32,51.
[10]	赵伟, 罗尧幸, 焦晓博, 韩凯, 刘榕晨, 李雪雪, 高美英, 纪薇. 葡萄中4个花发育相关MADS-box基因的表达与生物信息分析[J]. 分子植物育种, 2018, 16(20):6572-6582.doi:10.13271/j.mpb.016.006572. doi: 10.13271/j.mpb.016.006572
	Zhao W, Luo Y X, Jiao X B, Han K, Liu R C, Li X X, Gao M Y, Ji W. Expression and bioinformatics analysis of four MADS-box genes related to flower development in grape[J]. Molecular Plant Breeding, 2018, 16(20):6572-6582.
[11]	吴佳妮, 潘健, 郭春立, 吕铎, 何欢乐, 许桂梅, 蔡润, 潘俊松. 黄瓜不同性型花中差异表达的MADS-box基因[J]. 上海交通大学学报(农业科学版), 2017, 35(6):51-61.doi:10.3969/J.ISSN.1671-9964.2017.06.009. doi: 10.3969/J.ISSN.1671-9964.2017.06.009
	Wu J N, Pan J, Guo C L, Lü D, He H L, Xu G M, Cai R, Pan J S. MADS-box genes differentially expressed in diverse sex types in cucumber(Cucumis sativus L.)[J]. Journal of Shanghai Jiao Tong University (Agricultural Science), 2017, 35(6):51-61.
[12]	郭爽, 马宁, 杨文才, 沈火林. 辣椒花器官发育MADS-box基因的克隆与表达分析[J]. 园艺学报, 2010, 37(10):1591-1597.doi:10.16420/j.issn.0513-353x.2010.10.010. doi: 10.16420/j.issn.0513-353x.2010.10.010
	Guo S, Ma N, Yang W C, Shen H L. Cloning and expression analysis of a flowering-related MADS-box gene in pepper[J]. Acta Horticulturae Sinica, 2010, 37(10):1591-1597.
[13]	Xu Q K, Yu X Q, Cui Y J, Xia S S, Zeng D L, Qian Q, Ren D Y. LRG1 maintains sterile lemma identity by regulating OsMADS6 expression in rice[J]. Science China Life Sciences, 2021, 64(7):1190-1192.doi:10.1007/s11427-020-1816-x. doi: 10.1007/s11427-020-1816-x
[14]	丁献华, 陈伟, 张直峰. 荷花NnMADS6基因的分离与表达分析[J]. 分子植物育种, 2020, 18(7):2138-2145.doi:10.13271/j.mpb.018.002138. doi: 10.13271/j.mpb.018.002138
	Ding X H, Chen W, Zhang Z F. Isolation and expression analysis of the NnMADS6 gene in lotus[J]. Molecular Plant Breeding, 2020, 18(7):2138-2145.
[15]	Zhan J P. Beyond fruitful:Roles of tomato FRUITFULL-like genes in controlling inflorescence architecture and flowering[J]. The Plant Cell, 2022, 34(3):949-950.doi:10.1093/plcell/koab299. doi: 10.1093/plcell/koab299 URL
[16]	褚婷婷, 谢华, 徐勇, 马荣才. 拟南芥FRUITFULL(FUL)基因的表达调控模式[J]. 生物工程学报, 2010, 26(11):1546-1554.doi:10.13345/j.cjb.2010.11.006. doi: 10.13345/j.cjb.2010.11.006
	Chu T T, Xie H, Xu Y, Ma R C. Regulation pattern of the FRUITFULL(FUL)gene of Arabidopsis thaliana[J]. Chinese Journal of Biotechnology, 2010, 26(11):1546-1554.
[17]	Kinjo H, Shitsukawa N, Takumi S, Murai K. Diversification of three APETALA1/FRUITFULL-like genes in wheat[J]. Molecular Genetics and Genomics, 2012, 287(4):283-294.doi:10.1007/s00438-012-0679-7. doi: 10.1007/s00438-012-0679-7 URL
[18]	Immink R G, Hannapel D J, Ferrario S, Busscher M, Franken J, Lookeren Campagne M M, Angenent G C. A petunia MADS box gene involved in the transition from vegetative to reproductive development[J]. Development, 1999, 126(22):5117-5126.doi:10.1242/dev.126.22.5117. doi: 10.1242/dev.126.22.5117 pmid: 10529428
[19]	张良波, 王旋, 钱成旭, 刘志雄. 甜荞FeFUL2基因的克隆与表达分析[J]. 广西植物, 2021, 41(4):591-597.doi:10.11931/guihaia.gxzw201904025. doi: 10.11931/guihaia.gxzw201904025
	Zhang L B, Wang X, Qian C X, Liu Z X. Cloning and expression analysis of FeFUL2 gene from buckwheat(Fagopyrum esculentum)[J]. Guihaia, 2021, 41(4):591-597.
[20]	Jiang X B, Lubini G, Hernandes-Lopes J, Rijnsburger K, Veltkamp V, de Maagd R A, Angenent G C, Bemer M. FRUITFULL-like genes regulate flowering time and inflorescence architecture in tomato[J]. The Plant Cell, 2022, 34(3):1002-1019.doi:10.1093/plcell/koab298. doi: 10.1093/plcell/koab298 URL
[21]	Liu F, Yu H Y, Deng Y T, Zheng J Y, Liu M L, Ou L J, Yang B Z, Dai X Z, Ma Y Q, Feng S Y, He S, Li X F, Zhang Z Q, Chen W C, Zhou S D, Chen R, Liu M M, Yang S, Wei R M, Li H D, Li F, Ouyang B, Zou X X. PepperHub,an informatics hub for the chili pepper research community[J]. Molecular Plant, 2017, 10(8):1129-1132.doi:10.1016/j.molp.2017.03.005. doi: S1674-2052(17)30076-X pmid: 28343897

选择文件类型/文献管理软件名称

选择包含的内容

辣椒雄性不育系和保持系CaMADS6基因克隆及表达分析

Cloning and Expression Analysis of CaMADS6 in Cytoplasmic Male Sterile Line and Maintainer Line of Pepper

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 21

相关文章 15

编辑推荐

Metrics

本文评价

[1]	熊才运, 王阳, 裴虎, 莫海伟, 汤蕴琦, 黄君. *甜玉米雄性不育突变体ms2020的表型分析和基因定位*[J]. 华北农学报, 2023, 38(2): 14-20.
[2]	范依琳, 赵丹, 马妍, 岳永起, 冯欣欣, 张姬越, 字向东, 熊显荣, 付伟, 熊燕. *牦牛ANXA5基因的克隆、序列分析及表达研究*[J]. 华北农学报, 2023, 38(2): 224-231.
[3]	李潇, 郭文芳, 杨莉, 胡威, 匡柳青, 刘德春, 刘勇. *柑橘抗逆基因MYB96的克隆与表达分析*[J]. 华北农学报, 2023, 38(2): 57-64.
[4]	王鹏, 田哲娟, 赵雪芳, 康忱, 吴志明, 李亚栋, 黄冀楠. *番茄SlCaM3、SlCaM4和SlCaM5基因的克隆及低温胁迫下的表达分析*[J]. 华北农学报, 2023, 38(2): 75-84.
[5]	王莹, 王晓宇, 孙德慧, 霍红雁, 刘海臣, 徐惠, 张继星. 蓖麻钙依赖蛋白激酶29基因克隆与表达分析[J]. 华北农学报, 2023, 38(2): 85-92.
[6]	唐君, 代祥, 李贵飞, 穆兵, 王枫, 杨亦扬. *茶树NPF5基因克隆及其在不同氮素处理下的表达*[J]. 华北农学报, 2023, 38(2): 93-98.
[7]	王宏鹏, 曹高燚, 李明, 丁博, 包曙光, 王俊斌, 谢晓东, 陈小强. *大麦气孔发育转录因子基因HvMUTE的克隆及生物学功能初步鉴定*[J]. 华北农学报, 2023, 38(1): 40-45.
[8]	鲁丹丹, 谭政委, 余永亮, 李磊, 许兰杰, 杨红旗, 杨青, 董薇, 安素妨, 梁慧珍. *红花CtANR2和CtANR3基因的克隆、结构及表达模式分析*[J]. 华北农学报, 2023, 38(1): 84-93.
[9]	郭瑶晴, 孙晓靖, 连玉杰, 陈慧, 孙华越, 张雪海, 汤继华, 陈晓阳. *玉米雄性不育突变体x50的基因定位与遗传分析*[J]. 华北农学报, 2023, 38(1): 17-22.
[10]	吴军, 察艳艳, 李霞, 高亢. 大豆中4个NIN转录因子的生物信息学及表达分析[J]. 华北农学报, 2022, 37(S1): 26-34.
[11]	柳漆利, 张倩倩, 薛华丽, 毕阳, 宗元元, 彭慧. 粉红单端孢 TrPLD基因家族鉴定及其在侵染甜瓜过程中的表达[J]. 华北农学报, 2022, 37(6): 63-71.
[12]	胡子曜, 李秀青, 代培红, 雷建峰, 柳建飞, 赵燚, 邓嘉辉, 刘超, 刘晓东, 李月. *陆地棉细胞色素P450基因GhP450-94C1黄萎病抗性功能验证*[J]. 华北农学报, 2022, 37(6): 72-81.
[13]	阳文龙, 李锡香, 张晓辉, 宋江萍, 贾会霞, 王海平. 萝卜赤霉素生物合成和信号转导相关基因的全基因组鉴定及表达分析[J]. 华北农学报, 2022, 37(6): 1-14.
[14]	张曦, 钱玉源, 王广恩, 刘祎, 权月伟, 崔淑芳, 米换房, 李俊兰. *棉花色素腺体发育相关基因GhERF105-like的克隆与表达分析*[J]. 华北农学报, 2022, 37(6): 34-41.
[15]	金一锋, 高岩松, 王琦, 王萌萌, 赵迪, 熊毅, 陈阳. *草地早熟禾蛋白激酶SnRK2.4* 基因克隆及非生物胁迫响应分析**[J]. 华北农学报, 2022, 37(5): 9-15.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

辣椒雄性不育系和保持系CaMADS6基因克隆及表达分析

Cloning and Expression Analysis of CaMADS6 in Cytoplasmic Male Sterile Line and Maintainer Line of Pepper

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 21

相关文章 15

编辑推荐

Metrics

本文评价