基于转录组和代谢组解析香瓜茄果实类黄酮代谢差异

doi:10.7668/hbnxb.20193361

摘要/Abstract

摘要：

为了解香瓜茄果实主要类黄酮成分以及不同栽培种间类黄酮物质的代谢差异,以我国当前主栽的淡椭圆形果实(LOF)和甜圆形果实(SRF)2个香瓜茄栽培种类型为对象,对成熟果实进行转录组和代谢组联合分析。结果表明,香瓜茄果实中类黄酮组分共鉴定出9类二级代谢产物,41种三级代谢产物,黄酮醇含量最高。具有显著差异的代谢物有21种,主要分布在黄烷醇类、黄酮碳糖苷等6类二级代谢物质中。LOF的二氢黄酮、黄烷醇类、异黄酮的含量高于SRF,而黄酮醇等其他类黄酮化合物则在SRF中含量较高。RNA-seq分析共鉴定出与类黄酮代谢相关的基因503条,其中类黄酮合成通路上游的苯丙氨酸解氨酶(PAL)、肉桂醇脱氢酶(CAD)、黄烷酮-3-羟化酶(F3H)等关键基因表达在LOF中较SRF均上调,而黄酮醇合成酶基因(FLS)表达则在SRF中高于LOF。根据转录组的结果,4个类黄酮合成酶的表达趋势与RT-PCR的检测结果一致。研究结果表明,在不同香瓜茄资源LOF和SRF中存在大量的类黄酮化合物及其合成酶基因,但不同类黄酮成分及其合成代谢相关酶在不同资源含量均存在差异。

关键词: 香瓜茄, 类黄酮, 转录组, 代谢组

Abstract:

In order to understand the main flavonoids and the key synthase genes related to flavonoids and clarify the differences of flavonoids among different pepino.Combined metabolomic and transcriptomic analyses were carried out with the mature pepino(LOF and SRF)currently main cultivars in China.The results showed that 9 kinds of secondary metabolites and 41 kinds of tertiary metabolites were identified,and the flavonols content was the highest.There were 21 metabolites with significant differences in flavonoid components,which were distributed in 6 secondary metabolites such as flavanols,flavonoid carbonoside.The total content of dihydroflavonol,flavanols and isoflavones was higher in the Light-oval fruit(LOF)than in the Sweet-round fruit(SRF),while other compounds including flavonols were higher in the SRF.A total of 503 genes related to flavonoid synthesis were identified by RNA-seq analysis,the expression of Phenylalanine ammonia-lyase(PAL),Cinnamyl alcohol dehydrogenase(CAD),and Flavanone-3-Hydroxylase(F3H)which were key genes for upstream stage of the whole process of pepino flavonoid synthesis,were all up-regulated in LOF compared to SRF.Flavonol synthase gene expression was consistently higher in SRF than LOF.According to the result of transcriptome,the expressed trend of 4 flavonoid synthases agreed with the testing by the RT-PCR.The results showed that a large number of flavonoid compounds and their synthase genes were present in different cultivars of pepino.However,the content of different flavonoid components and the activities of enzymes related to its assimilation and is varied among different resources.

Key words: Pepino, Flavonoids, Transcriptome, Metabolome

司诚, 杨世鹏, 孙祝, 张广楠, 钟启文. 基于转录组和代谢组解析香瓜茄果实类黄酮代谢差异[J]. 华北农学报, 2023, 38(1): 53-62. doi: 10.7668/hbnxb.20193361.

SI Cheng, YANG Shipeng, SUN Zhu, ZHANG Guangnan, ZHONG Qiwen. Analysis of Flavonoid Metabolism in Different Cultivated Pepino Fruits Based on Transcriptome and Metabolome[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(1): 53-62. doi: 10.7668/hbnxb.20193361.

http://www.hbnxb.net/CN/Y2023/V38/I1/53

图/表 11

参考文献 32

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编码ID Code ID	基因名称 Gene name	引物序列(5'—3') Primer sequences
编码ID Code ID	基因名称 Gene name	F	R
Cluster-1244.24547	PAL	TGAAATCGCTATGGCCTCGT	TTTCAATATGTCAACTGCCTCC
Cluster-1244.48177	PAL	TGATGTTTGCACAATTCTCC	TTTGCCAAGAATTGAAGCTC
Cluster-1244.48397	F3H	CAACTATTTTGCCTGCTTC	GTATTCTTCACATGCCTCC
Cluster-1244.16099	FLS	TTACACTAGGATTGCCACCAC	TCTGCCATTGTTTTCATCTCG
Cluster-1244.3348	CAD	GATTCCTACATACAACGGCTA	AGTAATTCCAGCACATAACAGG
Cluster-2666.0	CAD	GCTACTCGGACATAATTGTTG	TTGTAATTCCAGCACAAAGCA
	内参基因Actin	GAACCGGAGCAGGTGAAGAA	GAAGCAATCCCAGCGATACG

编码ID Code ID	基因名称 Gene name	引物序列(5'—3') Primer sequences
编码ID Code ID	基因名称 Gene name	F	R
Cluster-1244.24547	PAL	TGAAATCGCTATGGCCTCGT	TTTCAATATGTCAACTGCCTCC
Cluster-1244.48177	PAL	TGATGTTTGCACAATTCTCC	TTTGCCAAGAATTGAAGCTC
Cluster-1244.48397	F3H	CAACTATTTTGCCTGCTTC	GTATTCTTCACATGCCTCC
Cluster-1244.16099	FLS	TTACACTAGGATTGCCACCAC	TCTGCCATTGTTTTCATCTCG
Cluster-1244.3348	CAD	GATTCCTACATACAACGGCTA	AGTAATTCCAGCACATAACAGG
Cluster-2666.0	CAD	GCTACTCGGACATAATTGTTG	TTGTAATTCCAGCACAAAGCA
	内参基因Actin	GAACCGGAGCAGGTGAAGAA	GAAGCAATCCCAGCGATACG

一级代谢产物 Primary metabolites	数量 Quantity	相对含量 Relative content
一级代谢产物 Primary metabolites	数量 Quantity	LOF	SRF
氨基酸及其衍生物 Amino acid and its derivatives	60	3.20E+08	2.34E+08
酚酸类 Phenolic acids	70	3.76E+08	4.33E+08
核苷酸及其衍生物 Nucleotide and its derivates	34	1.67E+08	8.58E+07
类黄酮 Flavonoids	41	1.15E+07	3.06E+07
醌类 Quinones	1	6.14E+03	3.62E+05
木脂素和香豆素 Lignans and coumarins	12	4.06E+06	2.65E+07
生物碱 Alkaloids	44	2.14E+08	1.98E+08
萜类 Terpene	1	1.19E+05	7.56E+04
有机酸 Organic acids	27	1.41E+08	1.73E+08
脂质 Lipid	68	8.66E+08	6.01E+08
其他 Others	44	7.63E+07	7.22E+07

一级代谢产物 Primary metabolites	数量 Quantity	相对含量 Relative content
一级代谢产物 Primary metabolites	数量 Quantity	LOF	SRF
氨基酸及其衍生物 Amino acid and its derivatives	60	3.20E+08	2.34E+08
酚酸类 Phenolic acids	70	3.76E+08	4.33E+08
核苷酸及其衍生物 Nucleotide and its derivates	34	1.67E+08	8.58E+07
类黄酮 Flavonoids	41	1.15E+07	3.06E+07
醌类 Quinones	1	6.14E+03	3.62E+05
木脂素和香豆素 Lignans and coumarins	12	4.06E+06	2.65E+07
生物碱 Alkaloids	44	2.14E+08	1.98E+08
萜类 Terpene	1	1.19E+05	7.56E+04
有机酸 Organic acids	27	1.41E+08	1.73E+08
脂质 Lipid	68	8.66E+08	6.01E+08
其他 Others	44	7.63E+07	7.22E+07

编码 Code	化合物 Chemical compound	二级代谢产物 Secondary metabolites	相对含量 Relative content		VIP
编码 Code	化合物 Chemical compound	二级代谢产物 Secondary metabolites	LOF	SRF	VIP
pmp000550	毛蕊黄酮苷	异黄酮	2.65E+04	1.69E+04
mws0024	没食子酸	黄烷醇类	8.42E+03	1.22E+04
mws0034	表没食子儿茶素没食子酸酯^*		9.89E+03	4.91E+03	1.24E+00
mws0351	没食子儿茶没食子酸酯		1.12E+04	5.94E+03
mws2220	没食子儿茶素没食子酸酯^*		1.44E+04	7.15E+03	1.16E+00
mws2221	儿茶素没食子酸酯^*		6.18E+03	2.03E+03	1.36E+00
pme0309	没食子酸甲酯		3.64E+03	3.05E+03
pmb0618	8-C-己糖基-橙皮素 O-己糖苷^*	黄酮碳糖苷	2.94E+03	1.71E+04	1.14E+00
pmb0645	6-C-己糖基-橙皮素 O-己糖苷		2.70E+04	2.53E+05
pmb0647	8-C-己糖基-木犀草素 O-戊糖苷^*		7.75E+02	9.99E+03	1.30E+00
pmp000410	葛根素芹菜糖苷^*		1.51E+03	6.62E+03	1.27E+00
mws0059	芦丁^*	黄酮醇	1.19E+04	8.06E+05	1.15E+00
mws1329	槲皮素-7-O-葡萄糖苷^*		5.18E+04	1.42E+05	1.11E+00
mws4183	槲皮素-3-O-Alpha-L-吡喃阿拉伯糖苷		7.91E+02	2.28E+03
pmb3894	3,7-二氧-甲基槲皮素^*		5.43E+06	3.08E+05	1.35E+00
pme1540	异鼠李素-3-O-新橙皮糖苷		2.76E+03	2.91E+04
pme3211	异槲皮甙^*		5.40E+04	1.69E+05	1.19E+00
pmn001583	槲皮素-3-O-刺槐甙^*		1.47E+04	1.00E+06	1.16E+00
pmn001642	山柰酚3-O-β-(2″-O-乙酰基-β-D-葡萄糖醛酸)		5.27E+06	3.55E+06
pmp001309	6-羟基山柰酚-7-O-葡萄糖苷^*		1.05E+04	1.78E+06	1.21E+00
pmp001310	6-羟基山柰酚-3,6-O-二葡萄糖苷^*		1.04E+05	2.15E+07	1.21E+00
pmp001312	6-羟基山柰酚-3,7,6-O-三葡萄糖苷^*		1.89E+03	3.67E+04	1.10E+00
pmp001314	6-羟基山柰酚-3-O-芸香糖-6-O-葡萄糖苷^*		9.32E+02	8.01E+04	1.17E+00
mws0043	川陈皮素	黄酮	3.12E+04	2.74E+04
pmb0588	木犀草素 3',7-二氧葡萄糖苷^*		4.28E+03	3.11E+04	1.22E+00
pmb0665	木犀草素 8-C-己糖苷-O-己糖苷^*		2.28E+03	8.99E+04	1.12E+00
pmb0725	麦黄酮 7-O-阿魏酰己糖苷		2.44E+04	1.13E+05
pmb1108	木犀草素 6-C-己糖基 8-C-己糖基-O-己糖苷		1.65E+03	3.04E+03
pmb2968	金圣草黄素 C-己糖基-O-己糖苷		8.05E+03	7.79E+03
pmp000001	高车前素^*		5.98E+02	1.99E+03	1.06E+00
pmp000593	木犀草素-7-O-芸香糖苷		3.75E+04	6.81E+03
mws0170	氯化矢车菊素,氯化氰定	花青素	1.27E+03	1.09E+04
pmb2957	矢车菊素 O-丁香酸		1.83E+05	3.08E+05
mws0914	短叶松素	二氢黄酮醇	7.54E+02	2.68E+04
pmb2979	橙皮素 O-丙二酰基己糖苷^*		2.36E+04	1.03E+05	1.07E+00
mws0064	圣草酚^*	二氢黄酮	6.23E+04	3.97E+03	1.26E+00
mws1033	高圣草酚^*		8.87E+03	3.07E+03	1.01E+00
pme0376	柚皮素		5.71E+02	2.66E+04
pmp001075	橙皮素	查尔酮	5.29E+02	9.76E+03
mws1140	4,2',4',6'-四羟基查尔酮		8.49E+02	2.81E+04
pme2960	柚皮苷查尔酮		8.03E+02	7.36E+04

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