华北农学报 ›› 2024, Vol. 39 ›› Issue (4): 54-63. doi: 10.7668/hbnxb.20194874

所属专题: 油料作物 生物技术

• 作物遗传育种·种质资源·生物技术 • 上一篇    下一篇

甘蓝型油菜抗茎秆倒伏鉴定与候选基因挖掘

吴心成, 贺日升, 肖硕定, 张振乾, 杨柳, 刘忠松, 陈浩   

  1. 湖南农业大学 农学院,湖南 长沙 410000
  • 收稿日期:2024-01-26 出版日期:2024-08-27
  • 通讯作者:
    陈 浩(1987—),男,湖南长沙人,讲师,博士,主要从事油菜遗传育种研究。
  • 作者简介:

    吴心成(1998—),男,湖南岳阳人,硕士,主要从事油菜遗传育种研究。

  • 基金资助:
    国家重点研发计划项目(2023YFD1201400)

Identification and Candidate Gene Mining of Stem Lodging Resistance in Brassica napus

WU Xincheng, HE Risheng, XIAO Shuoding, ZHANG Zhenqian, YANG Liu, LIU Zhongsong, CHEN Hao   

  1. College of Agronomy,Hunan Agricultural University,Changsha 410000,China
  • Received:2024-01-26 Published:2024-08-27

摘要:

为促进甘蓝型油菜抗倒伏品种的培育,挖掘甘蓝型油菜茎秆抗倒伏的基因资源,以4份抗倒伏和3份易倒伏种质为材料,取其盛花期下部茎秆测定纤维素、半纤维素、总果胶、原果胶和木质素等5种组分的含量,并对YLS0084(抗倒伏)和YLS1691(易倒伏)的下部茎秆进行转录组测序分析。结果发现,抗倒材料的纤维素和总果胶平均含量极显著高于易倒伏材料;转录组分析共挖掘到7 397个表达量上调和9 438个表达量下调的差异表达基因,这些差异表达基因富集在碳水化合物代谢、翻译、氨基酸代谢和信号转导等通路;通过qRT-PCR验证了9个与油菜抗倒伏相关的基因(BnaA01G0071800ZSBnaA01G0175700ZSBnaA01G0205800ZSBnaA03G0404800ZSBnaA03G0517200ZSBnaA05G0431400ZSBnaA07G0056300ZSBnaA09G0031300ZSBnaC05G0128400ZS)在YLS0084中显著上调表达。研究结果证明了甘蓝型油菜茎秆中纤维素和总果胶的含量对抗倒伏起积极作用,并为甘蓝型油菜抗倒伏提供了一定的重要基因资源。

关键词: 甘蓝型油菜, 抗茎秆倒伏, 纤维素, 果胶, 转录组分析

Abstract:

In order to promote the cultivation of lodging resistant varieties of Brassica napus and explore the genetic resources of lodging resistance in the stem of Brassica napus,this study used four lodging resistant and three easily lodging resistant germplasm as materials,and measured the content of five components,including cellulose,hemicellulose,total pectin,protopectin,and lignin,in the lower stem during the flowering period.Transcriptome sequencing analysis was performed on the lower stem of YLS0084(lodging resistant)and YLS1691(easily lodging prone).The results showed that the average content of cellulose and total pectin in the anti toppling material,significantly higher than materials prone to lodging;transcriptome analysis revealed a total of 7 397 differentially expressed genes with upregulation and 9 438 downregulation,which were enriched in pathways such as carbohydrate metabolism,translation,amino acid metabolism,and signal transduction;nine genes related to lodging resistance in rapeseed(BnaA01G0071800ZS,BnaA01G0175700ZS,BnaA01G0205800ZS,BnaA03G0404800ZS,BnaA03G0517200ZS,BnaA05G0431400ZS,BnaA07G0056300ZS,BnaA09G0031300ZS,and BnaC05G0128400ZS)were validated through qRT-PCR,and were significantly upregulated in YLS0084.The results of this study demonstrates that the content of cellulose and total pectin in the stem of Brassica napus has a positive effect on lodging resistance and provides important genetic resources for lodging resistance in Brassica napus.

Key words: Brassica napus, Stem lodging resistance, Cellulose, Pectin, Transcriptome analysis

引用本文

吴心成, 贺日升, 肖硕定, 张振乾, 杨柳, 刘忠松, 陈浩. 甘蓝型油菜抗茎秆倒伏鉴定与候选基因挖掘[J]. 华北农学报, 2024, 39(4): 54-63. doi: 10.7668/hbnxb.20194874.

WU Xincheng, HE Risheng, XIAO Shuoding, ZHANG Zhenqian, YANG Liu, LIU Zhongsong, CHEN Hao. Identification and Candidate Gene Mining of Stem Lodging Resistance in Brassica napus[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 54-63. doi: 10.7668/hbnxb.20194874.