华北农学报 ›› 2024, Vol. 39 ›› Issue (4): 47-53. doi: 10.7668/hbnxb.20194546

所属专题: 油料作物 盐碱胁迫 生物技术 热点论文

• 作物遗传育种·种质资源·生物技术 • 上一篇    下一篇

甘蓝型油菜转录因子BnHY5-2参与盐碱胁迫的响应

杨明煊1, 李明玉1, 汪波2, 王泽1, 刘智强3, 周广生2, 于放1, 刘志文1   

  1. 1 大连工业大学 生物工程学院,辽宁 大连 116034
    2 农业农村部长江中游作物生理生态与耕作重点实验室,华中农业大学 植物科学技术学院,湖北 武汉 430070
    3 大连市农业科学研究院,辽宁 大连 116036
  • 收稿日期:2024-04-19 出版日期:2024-08-27
  • 通讯作者:
    刘志文(1972—),男,湖北罗田人,副教授,博士,硕士生导师,主要从事作物的逆境生理和分子生物学研究。
  • 作者简介:

    杨明煊(2001—),男,河南焦作人,在读硕士,主要从事作物逆境生理研究。

  • 基金资助:
    作物遗传改良国家重点实验室开放基金(20200902); 辽宁省教育厅科学研究项目(LJKZ0523); 大连工业大学国家级大学生创新创业项目(202310152015)

Response of the Transcription Factor BnHY5-2 in Brassica napus L.to Salt and Alkali Stress

YANG Mingxuan1, LI Mingyu1, WANG Bo2, WANG Ze1, LIU Zhiqiang3, ZHOU Guangsheng2, YU Fang1, LIU Zhiwen1   

  1. 1 School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China
    2 MARA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China
    3 Dalian Academy of Agricultural Sciences,Dalian 116036,China
  • Received:2024-04-19 Published:2024-08-27

摘要:

转录因子BnHY5-2与植物抗逆性相关。为揭示甘蓝型油菜转录因子BnHY5-2对盐碱胁迫的响应,主要通过瞬时转化过表达、qRT-PCR分析、亚细胞定位等方法,分析甘蓝型油菜转录因子BnHY5-2对光和盐碱的响应。结果表明,光照处理油菜后,在叶与茎中BnHY5-2基因的表达量均显著高于黑暗条件下,分别为黑暗条件下的29.22,3.15倍,叶片对光的敏感性大于茎,说明叶片是响应光转录因子BnHY5-2的主要部位。在光照条件下应用大连滨海盐碱土种植油菜,处理后BnHY5-2的表达在叶和茎中均显著下调,分别下调53.1%,31.0%;在黑暗条件下应用盐碱处理后BnHY5-2的表达在茎中下调48.2%,而在叶中的表达差异则不显著,表现出器官的差异性,说明叶片对光照条件要求更加严格。在油菜叶片中瞬时过表达BnHY5-2基因时,6个与盐碱相关的候选基因的表达中,有2个(BnNAC32BnGS)上调表达,分别为原来的1.25,3.28倍;而有4个(BnamyBnAspBnNHX7BnTPS)下调表达,分别下降24.8%,25.4%,71.0%,82.0%,同时甜菜碱含量由0.256 mg/g提升至0.573 mg/g,提高了1.24倍,说明过表达BnHY5-2基因会提高油菜的盐碱抗性。亚细胞定位结果显示,转录因子BnHY5-2定位于细胞核中,调控功能基因的表达。因此,BnHY5-2不仅与光信号有关,还参与油菜盐碱抗性。

关键词: 甘蓝型油菜, BnHY5-2, 盐碱胁迫, 过表达, 转录因子

Abstract:

The transcription factor BnHY5-2 is associated with plant stress resistance.In order to reveal the response of Brassica napus L.transcription factor BnHY5-2 to salt alkali stress in B.napus,the response of BnHY5-2 to light and salt and alkali was analyzed by transient overexpression,qRT-PCR analysis and subcellular localization.The results revealed that under light conditions,the expression level of the BnHY5-2 gene in B.napus leaves and stems was 29.22 and 3.15 fold higher,respectively,compared to dark conditions.The higher sensitivity to light in leaves suggested that they were the primary site for light signal response.Under light conditions,the expression of BnHY5-2 in leaves and stems was significantly downregulated by 53.1% and 31.0%,respectively,when B.napus was planted in Dalian coastal saline-alkali soil;after applying saline-alkali treatment under dark conditions,the expression of BnHY5-2 was downregulated by 48.2% in the stem,while the difference in expression in the leaves was not significant,indicating organ differences,indicating that the leaves had stricter requirements for light conditions.In B.napus leaves with transient overexpression of BnHY5-2,two out of six genes related to saline-alkali stress(BnNAC32 and BnGS)showed upregulation by 1.25,3.28 fold,respectively,while the other four genes(Bnamy,BnAsp,BnNHX7,BnTPS)were downregulated by 24.8%,25.4%,71.0%,and 82.0%,respectively.Meanwhile,the content of the resistance substance betaine in B.napus increased from 0.256 to 0.573 mg/g,indicating an enhancement by 1.24 fold,suggesting that the overexpression of BnHY5-2 gene could improve the saline-alkali tolerance of B.napus.Subcellular localization results showed that the transcription factor BnHY5-2 was localized in the nucleus and regulates the expression of functional genes.Therefore,BnHY5-2 is not only related to light signaling but also participates in the saline-alkali resistance of Brassica napus L.

Key words: Brassica napus L., BnHY5-2, Saline-alkali stress, Overexpression, Transcription factor

引用本文

杨明煊, 李明玉, 汪波, 王泽, 刘智强, 周广生, 于放, 刘志文. 甘蓝型油菜转录因子BnHY5-2参与盐碱胁迫的响应[J]. 华北农学报, 2024, 39(4): 47-53. doi: 10.7668/hbnxb.20194546.

YANG Mingxuan, LI Mingyu, WANG Bo, WANG Ze, LIU Zhiqiang, ZHOU Guangsheng, YU Fang, LIU Zhiwen. Response of the Transcription Factor BnHY5-2 in Brassica napus L.to Salt and Alkali Stress[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 47-53. doi: 10.7668/hbnxb.20194546.