Zn和EDTA对紫花苜蓿叶片光合特性的响应

doi:10.7668/hbnxb.2016.02.019

华北农学报 ›› 2016, Vol. 31 ›› Issue (2): 114-119. doi: 10.7668/hbnxb.2016.02.019

Zn和EDTA对紫花苜蓿叶片光合特性的响应

代惠萍^1,2,3, 赵桦², 贾根良⁴, 冯佰利⁵

1. 陕西理工学院, 陕西省资源重点实验室, 陕西汉中 723001;
2. 陕西理工学院生物科学与工程学院, 陕西汉中 723001;
3. 兰州大学生命科学学院, 甘肃省环境生物监测与修复重点实验室, 甘肃兰州 730000;
4. 西北农林科技大学理学院, 陕西杨凌 712100;
5. 西北农林科技大学, 旱区作物逆境生物学国家重点实验室, 陕西杨凌 712100

收稿日期:2016-02-14 出版日期:2016-04-28
作者简介:代惠萍(1972-),女,陕西武功人,副教授,博士,主要从事植物逆境生理学、植被恢复理论与技术研究。
基金资助:
陕西省教育厅重点项目(15JS019);陕西省自然基金项目(2015JM3086);甘肃省环境生物监测与修复重点实验室开放基金项目(GBBL2015006);国家大学生创新创业训练计划项目(201510720570);陕西省大学生创新创业训练计划项目(1970);陕西理工学院大学生创新创业训练计划项目(UIRP15034);国家大学生创新创业训练计划项目(201510720570)

Responses to Zn and EDTA Chelating Agents on Photosynthesis Characteristics of Alfalfa Leaves

DAI Huiping^1,2,3, ZHAO Hua², JIA Genliang⁴, FENG Baili⁵

1. Shaanxi University of Technology, Shaanxi Province Key Laboratory of Bio-resources, Hanzhong 723001, China;
2. College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China;
3. College of Life Sciences, Lanzhou University, Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Lanzhou 730000, China;
4. College of Science, Northwest A & F University, Yangling 712100, China;
5. Northwest A & F University, State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China

Received:2016-02-14 Published:2016-04-28

摘要/Abstract

摘要： 为了探索提高紫花苜蓿的抗锌能力,以3个紫花苜蓿品种为试材,采用土培法,研究Zn胁迫(250μg/kg)下,EDTA对不同品种紫花苜蓿叶片锌富集量、光合色素含量及叶绿素荧光参数的影响。结果表明,在50 d时,紫花苜蓿叶片Zn的含量均显著增高,Zn+EDTA显著提高了苜蓿叶片对锌的吸收;大叶苜蓿品种叶片光合色素的含量高于对照,大叶苜蓿叶绿素a、叶绿素b、总叶绿素和类胡萝卜素的含量显著高于其他2个品种;EDTA处理可提高大叶苜蓿初始荧光值、可变荧光、最大荧光值、最大光化学效率的水平。结果显示,EDTA促进大叶苜蓿叶片的光合色素含量的增加,减少过剩激发能的耗散,提高光合电子传递效率,有效降低锌胁迫对紫花苜蓿叶片PSⅡ的伤害,提高大叶苜蓿的抗锌能力。

关键词: 锌, EDTA, 紫花苜蓿, 叶绿素, 叶绿荧光

Abstract: In order to explore the effective avenue on improving alfalfa(Medicage sativa L.) resistant ability under zinc stress, pot experiments were used to study the effect of EDTA on zinc accumulation, photosynthetic pigment content and chlorophyll fluorescence under zinc stress in different alfalfa leaves of three cultivars Medicago sativa ssp., Vitoria Alfalfa and Medicago sativa cv..The results of this study indicated that the zinc contents in three cultivar leaves were significantly higher than that in control group.Compared with zinc stress alone, the addition of EDTA strikingly enhanced the zinc absorption in leaves.In comparison with different cultivars, photosynthetic pigment content in Medicago sativa ssp.was higher than that in control group.After EDTA treatment, the contents of chlorophyll a, chlorophyll b, total chlorophyll and carotenoids were 12.7%, 11.8%, 12.5%, 14.8% of Medicago sativa ssp.respectively, which were remarkably higher than that in the other two cultivars.Fo, Fv, Fm and Fv/Fm levels were increased by 5.4%, 14.9%, 18.1%, 4.0% of Medicago sativa ssp.with the treatment of EDTA.Our research indicated that EDTA was able to promote the accumulation of photosynthetic pigment, reduce the dissipation of excessive excitation energy, improve the transmission efficiency of photosynthetic electron, and minimize the damages to alfalfa leaves PSⅡ caused by zinc stress effectively, all of which coherently boosted resistance capability to zinc in Medicago sativa ssp.cultivar.As the results suggested, EDTA with the appropriate concentration at 10 μg could coefficient alleviate the damage of Zinc stress to Medicago sativa ssp., reduce dissipation of excess excitation and improve photochemical electron transport efficiency and efficiently protect from PSⅡ damage, EDTA obviously promoted the Zinc resistance of Medicago sativa ssp.under Zinc stress.

Key words: Zinc, Ethylene diamine tetraacetic acid, Medicage sativa L., Chlorophyll, Chlorophyll fluorescence

中图分类号:

S551.01

代惠萍, 赵桦, 贾根良, 冯佰利. Zn和EDTA对紫花苜蓿叶片光合特性的响应[J]. 华北农学报, 2016, 31(2): 114-119. doi: 10.7668/hbnxb.2016.02.019.

DAI Huiping, ZHAO Hua, JIA Genliang, FENG Baili. Responses to Zn and EDTA Chelating Agents on Photosynthesis Characteristics of Alfalfa Leaves[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 114-119. doi: 10.7668/hbnxb.2016.02.019.

http://www.hbnxb.net/CN/Y2016/V31/I2/114

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