氮锌配施对夏玉米籽粒矿质元素含量和累积量的影响

doi:10.7668/hbnxb.20193757

摘要/Abstract

摘要：

研究氮锌配施下夏玉米籽粒矿质元素含量的变化,为玉米生产上氮锌肥的合理施用提供参考。采用大田小区试验,以郑单958和谷神玉66为材料,设置90,180,225 kg/hm²等3个施氮水平和不喷锌、苗期和拔节期1∶1喷锌、拔节期和大口期1∶1喷锌、大口期喷锌等4个喷锌处理,分析氮锌配施对夏玉米籽粒产量、矿质元素含量和累积量的影响。结果表明,施氮量超过180 kg/hm²,籽粒产量显著下降,而籽粒中Ca、Cu、Fe、Zn含量以及Cu和Fe累积量显著增加,施氮量为180 kg/hm²,N含量和P、K、Mg含量分别达最高和最低;拔节期和大口期1∶1喷锌和大口期喷锌2个处理均能显著提高籽粒产量、N和Zn含量以及N、Mg、Zn、B和Na累积量,但降低了P、K、Ca、B和Na含量;与谷神玉66相比,郑单958产量平均提高了19.3%,且籽粒中K和Fe含量以及K、Ca、B和Na累积量也显著提高。籽粒产量与大多矿质元素含量呈极显著负相关,除Ca与Mg和Zn相关性不显著外,籽粒中Ca、Mg、Cu、Mn、Fe、Zn和B含量之间均达显著或极显著正相关,Zn与N、Mg含量的回归关系均达显著或极显著水平。总之,施用氮肥180 kg/hm²,结合拔节期大口期1∶1叶面喷施锌肥4.5 kg/hm²,能够使玉米籽粒高产稳产,并同步提高籽粒中N和Zn含量及其他矿质元素累积量,达到籽粒产量和矿质元素营养品质同步提高的目的,可在大田中进行推广应用。

关键词: 夏玉米, 氮锌配施, 矿质元素, 含量, 累积量, 产量

Abstract:

It aimed to research the variation of mineral elements in the maize grain under the nitrogen(N)and zinc(Zn)fertilization,in order to provide scientific references for N and Zn application in the maize production.In the field plot experiment,two varieties including ZD958 and GSY66 were fertilized with three N rates(90,180 and 225 kg/ha)and four Zn treatments(no Zn,spraying Zn of 1∶1 at seedling stage and jointing stage,spraying Zn of 1∶1 at jointing stage and flare opening stage and spraying Zn at flare opening stage),to analyze the effects of N and Zn fertilization on the maize grain yield and mineral elements concentration and accumulation.The results showed that grain yield was significantly decreased,while Ca,Cu,Fe and Zn concentration as well as Cu and Fe accumulation were significantly increased when the N rate was more than 180 kg/ha.N and P,K,Mg concentration achieved the highest and lowerest values respectively under the 180 kg/ha N treatment.Spraying Zn of 1∶1 at jointing stage and flare opening stage and at flare opening stage only could significantly improve the yield,N and Zn concentration and N,Mg,Zn,B and Na accumulation,but decrease the P,K,Ca,B and Na concentration.Relative to GSY66,the grain yield of ZD958 was significantly increased by 19.3%,and K and Fe concentration and K,Ca,B and Na accumulation were also significantly increased.Grain yield showed extremely significantly negative correlation ship with most mineral element concentration.The positive correlationships among Ca,Mg,Cu,Mn,Fe,Zn and B were significant or extremely significant,expect for Ca and Mg,Zn.The linear regression relationships between grain Zn concentration and N,Mg concentration were significant or extremely significant.To sum up,applying 180 kg/ha N and spraying 4.5 kg/ha Zn of 1∶1 at jointing stage and flare opening stage could increase maize grain yield,N and Zn concentration and mineral elements accumulation,synchronously achieve the high yield and mineral nutrition,and are recommended to promote in maize production.

Key words: Summer maize, N and Zn application, Mineral element, Concentration, Accumulation, Yield

张盼盼, 乔江方, 李川, 张美微, 牛军. 氮锌配施对夏玉米籽粒矿质元素含量和累积量的影响[J]. 华北农学报, 2023, 38(3): 87-99. doi: 10.7668/hbnxb.20193757.

ZHANG Panpan, QIAO Jiangfang, LI Chuan, ZHANG Meiwei, NIU Jun. Effect of Nitrogen and Zinc Application on the Concentration and Accumulation of Mineral Elements in the Grain of Summer Maize[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(3): 87-99. doi: 10.7668/hbnxb.20193757.

http://www.hbnxb.net/CN/Y2023/V38/I3/87

图/表 9

参考文献 33

[1]	国家统计局, 生态环境部. 中国环境统计年鉴-2021[M]. 北京: 中国统计出版社, 2021.
	National Bureau of Statistics. China statistical yearbook 2021[M]. Beijing: China Statistics Press, 2021.
[2]	Liu D Y, Zhang W, Yan P, Chen X P, Zhang F S, Zou C Q. Soil application of zinc fertilizer could achieve high yield and high grain zinc concentration in maize[J]. Plant and Soil, 2017, 411(1/2):47-55.doi:10.1007/s11104-016-3105-9. doi: 10.1007/s11104-016-3105-9 URL
[3]	Zhang J Y, Wang S F, Song S H, Xu F S, Pan Y H, Wang H. Transcriptomic and proteomic analyses reveal new insight into chlorophyll synthesis and chloroplast structure of maize leaves under zinc deficiency stress[J]. Journal of Proteomics, 2019, 199:123-134.doi:10.1016/j.jprot.2019.03.001. doi: S1874-3919(19)30074-0 pmid: 30849524
[4]	Stanković M, Topuzović M, Marković A, Pavlović D, D-elić G, Bojović B, Branković S. Influence of zinc(Zn)on germination of wheat(Triticum aestivum L.)[J]. Biotechnology & Biotechnological Equipment, 2010, 24(2):236-239.doi:10.1080/13102818.2010.10817842. doi: 10.1080/13102818.2010.10817842
[5]	Xue Y F, Xia H Y, Christie P, Zhang Z, Li L, Tang C X. Crop acquisition of phosphorus,iron and zinc from soil in cereal/legume intercropping systems:A critical review[J]. Annals of Botany, 2016, 117(3):363-377.doi:10.1093/aob/mcv182. doi: 10.1093/aob/mcv182 URL
[6]	Guo S, Chen Y H, Chen X C, Chen Y L, Yang L, Wang L F, Qin Y S, Li M S, Chen F J, Mi G H, Gu R L, Yuan L X. Grain mineral accumulation changes in Chinese maize cultivars released in different decades and the responses to nitrogen fertilizer[J]. Front Plant Sci, 2019, 10:1662.doi:10.3389/fpls.2019.01662. doi: 10.3389/fpls.2019.01662 pmid: 31993065
[7]	薄其飞. 旱作玉米籽粒微量元素对品种、覆盖和施肥措施的响应[D]. 杨凌: 西北农林科技大学, 2022.
	doi:10.27409/d.cnki.gxbnu.2022.00046. Bo Q F.Response of trace elements in dry-farming maize seeds to varieties,mulching and fertilization measures[D]. Yangling: Northwest A&F University, 2022.
[8]	王朔林, 王改兰, 杨艳菊, 赵旭, 陈春玉, 黄学芳. 长期施肥对玉米籽粒中植物必需营养元素含量的影响[J]. 华北农学报, 2014, 29(2):208-212.doi:10.3969/j.issn.1004-7484(x).2012.12.536. doi: 10.7668/hbnxb.2014.02.039
	Wang S L, Wang G L, Yang Y J, Zhao X, Chen C Y, Huang X F. Effects of long-term fertilization on contents of plant essential nutrient in maize grain[J]. Acta Agriculturae Boreali-Sinica, 2014, 29(2):208-212.
[9]	Zhang P P, Ma G, Wang C Y, Zhu Y J, Guo T C. Mineral elements bioavailability in milling fractions of wheat grain response to zinc and nitrogen application[J]. Agronomy Journal, 2019, 111(5):2504-2511.doi:10.2134/agronj2019.02.0104. doi: 10.2134/agronj2019.02.0104 URL
[10]	蒋曦龙, 王澜, 乔月彤, 李晓靖, 薛燕慧, 陈利容, 夏海勇. 叶面喷锌对两种类型玉米产量、籽粒锌等矿质营养元素含量的影响[J]. 山东农业科学, 2021, 53(3):72-78.doi:10.14083/j.issn.1001-4942.2021.03.013. doi: 10.14083/j.issn.1001-4942.2021.03.013
	Jiang X L, Wang L, Qiao Y T, Li X J, Xue Y H, Chen L R, Xia H Y. Effects of foliar spraying zinc on yield and contents of zinc and other mineral elements of two maize cultivars[J]. Shandong Agricultural Sciences, 2021, 53(3):72-78.
[11]	李文宗, 张兰, 徐妙云, 王磊. 富锌玉米的筛选及叶面喷施锌肥对玉米籽粒中矿物元素的影响分析[J]. 中国农业科技导报, 2018, 20(1):47-54.doi:10.13304/j.nykjdb.2017.0098. doi: 10.13304/j.nykjdb.2017.0098
	Li W Z, Zhang L, Xu M Y, Wang L. Screening of zinc-rich corn and analysis of the effect of spraying zinc fertilizer on mineral elements in corn grains[J]. Journal of Agricultural Science and Technology, 2018, 20(1):47-54.
[12]	杨习文, 宋淼, 李秋杰, 周苏玫, 韩少宇, 陈旭, 徐利利, 贺德先. 氮锌配施对小麦锌转运、分配与累积的影响[J]. 应用生态学报, 2020, 31(1):148-156.doi:10.13287/j.1001-9332.202001.027. doi: 10.13287/j.1001-9332.202001.027
	Yang X W, Song M, Li Q J, Zhou S M, Han S Y, Chen X, Xu L L, He D X. Impacts of combined N and Zn application on Zn translocation,partitioning,and accumulation in Triticum aestivum[J]. Chinese Journal of Applied Ecology, 2020, 31(1):148-156.
[13]	李宇航, 杜天庆, 龚芮, 杨舒添, 翟红梅, 崔福柱, 孙敏, 高志强. 叶面喷锌对糯玉米生理特性、籽粒锌含量及产量的影响[J]. 河南农业科学, 2020, 49(10):26-32.doi:10.15933/j.cnki.1004-3268.2020.10.004.
	Li Y H, Du T Q, Gong R, Yang S T, Zhai H M, Cui F Z, Sun M, Gao Z Q. Effects of foliar application of zinc on physiological characteristics,grain Zn content and yield of waxy corn[J]. Journal of Henan Agricultural Sciences, 2020, 49(10):26-32.
[14]	黄伟东. 锌-硼喷施对寒地玉米生理特性及产量品质的影响[D]. 大庆: 黑龙江八一农垦大学, 2020.
	doi:10.27122/d.cnki.ghlnu.2020.000280. Huang W D.Effects of zinc-boron spraying on physiological characteristics, yield and quality of maize in cold region[D]. Daqing: Heilongjiang Bayi Agricultural University, 2020.
[15]	刘智龙. 土施和叶面喷锌对玉米锌吸收和分布的影响研究[D]. 长春: 吉林农业大学, 2012.
	Liu Z L. Effects of soil application and foliar spraying zinc on zinc absorption and distribution in maize[D]. Changchun: Jinlin Agricultural University, 2012.
[16]	赵海蓓. 锌肥和几种生长调节物质对玉米产量和品质的影响[D]. 石河子: 石河子大学, 2020.doi:10.27332/d.cnki.gshzu.2020.000743.
	Zhao H B. Effects of zinc fertilizer and several growth regulators on yield and quality of maize[D]. Shihezi: Shihezi University, 2020.
[17]	薛艳芳, 张慧, 刘开昌, 夏海勇, 高英波, 王庆成, 温立玉, 孔玮琳, 张虎虎, 付希强, 齐世军, 李宗新. 黄淮海区主推玉米品种子粒主要矿物质元素含量分析[J]. 玉米科学, 2017, 25(5):56-62.doi:10.13597/j.cnki.maize.science.20170509. doi: 10.13597/j.cnki.maize.science.20170509
	Xue Y F, Zhang H, Liu K C, Xia H Y, Gao Y B, Wang Q C, Wen L Y, Kong W L, Zhang H H, Fu X Q, Qi S J, Li Z X. Analysis of major elements in grain of the popularized maize varieties in Huanghuaihai plain[J]. Journal of Maize Sciences, 2017, 25(5):56-62.
[18]	毛红艳, 于明, 祖力皮牙·买买提.微波消解-ICP-AES法测定新疆玉米矿物元素含量及主成分分析[J]. 新疆农业科学, 2022, 59(5):1270-1276.doi:10.6048/j.issn.1001-4330.2022.05.028. doi: 10.6048/j.issn.1001-4330.2022.05.028
	Mao H Y, Yu M, Zu L P Y. Determination of mineral elements in maize cultivars by microwave digestion/ICP-AES and principal component analysis[J]. Xinjiang Agricultural Sciences, 2022, 59(5):1270-1276. doi: 10.6048/j.issn.1001-4330.2022.05.028
[19]	Šimić D, Sudar R, Ledencˇan T, Jambrović A, Zdunić Z, Brkić I, Kovacˇević V. Genetic variation of bioavailable iron and zinc in grain of a maize population[J]. Journal of Cereal Science, 2009, 50(3):392-397.doi:10.1016/j.jcs.2009.06.014. doi: 10.1016/j.jcs.2009.06.014 URL
[20]	陈洁, 潘光堂, 李华雄, 杨克诚, 王康宁, 谢传晓, 张世煌, 张谦. 玉米种质籽粒铁含量的测定及差异分析[J]. 西南农业学报, 2007, 20(6):1198-1201.doi:10.3969/j.issn.1001-4829.2007.06.012. doi: 10.3969/j.issn.1001-4829.2007.06.012
	Chen J, Pan G T, Li H X, Yang K C, Wang K N, Xie C X, Zhang S H, Zhang Q. The test and difference analysis of the iron content in maize germplasm seeds[J]. Southwest China Journal of Agricultural Sciences, 2007, 20(6):1198-1201.
[21]	黄玉芳, 叶优良, 陈文莉, 任宁, 赵亚南, 汪洋. 氮肥用量对玉米籽粒主要矿质元素质量分数的影响[J]. 西北农业学报, 2020, 29(6):870-876.doi:10.7606/j.issn.1004-1389.2020.06.007 doi: 10.7606/j.issn.1004-1389.2020.06.007
	Huang Y F, Ye Y L, Chen W L, Ren N, Zhao Y N, Wang Y. Effects of nitrogen application rates on mineral mass fractions of maize grain[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2020, 29(6):870-876.
[22]	薛艳芳. 氮肥管理对高产小麦和玉米锌吸收、转移与累积的影响[D]. 北京: 中国农业大学, 2014.
	Xue Y F.Effect of nitrogen management on zinc uptake,translocation and allocation in high-yielding wheat(Triticum aestivum L.) and maize(Zea mays L.)cropping system[D]. Beijing: China Agricultural University, 2014.
[23]	韩证仿. 东北不同年代玉米品种籽粒主要矿质元素含量及其对氮肥的响应[D]. 北京: 中国农业科学院, 2012.
	Han Z F. Contents of main mineral elements in grains of maize varieties in different ages in Northeast China and their responses to nitrogen fertilizer[D]. Beijing: Chinese Academy of Agricultural Sciences, 2012.
[24]	王建伟. 硒锌对典型旱地主要作物产量及矿质营养的影响[D]. 杨凌: 西北农林科技大学, 2013.
	Wang J W. Effects of selenium and zinc on yield and mineral nutrition of main crops in typical dryland[D]. Yangling: Northwest A&F University, 2013.
[25]	展文洁, 刘永权, 袁静超, 张洪喜, 张水梅, 刘剑钊, 任军. 黑钙土增施中微量元素对玉米生长的影响[J]. 东北农业科学, 2021, 46(1):47-51.doi:10.16423/j.cnki.1003-8701.2021.01.012. doi: 10.16423/j.cnki.1003-8701.2021.01.012
	Zhan W J, Liu Y Q, Yuan J C, Zhang H X, Zhang S M, Liu J Z, Ren J. Study on the status of trace elements in chernozem on maize growth[J]. Journal of Northeast Agricultural Sciences, 2021, 46(1):47-51.
[26]	赵海蓓, 吕小凡, 白如霄, 张新疆, 孙帅, 危常州. 锌肥喷施时期和次数对塔城地区玉米生长发育及产量的影响[J]. 西北农业学报, 2020, 29(11):1651-1659.doi:10.7606/j.issn.1004-1389.2020.11.006. doi: 10.7606/j.issn.1004-1389.2020.11.006
	Zhao H B, Lü X F, Bai R X, Zhang X J, Sun S, Wei C Z. Effects of zinc foliar spraying time and frequency on maize growth and yield in Tacheng area[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2020, 29(11):1651-1659.
[27]	张盼盼, 乔江方, 李毓珍, 何冠华, 李川, 张美微, 牛军. 喷锌时期对不同夏玉米品种籽粒产量及锌含量的影响[J]. 河南农业科学, 2022, 51(6):22-31.doi:10.15933/j.cnki.1004-3268.2022.06.003. doi: 10.15933/j.cnki.1004-3268.2022.06.003
	Zhang P P, Qiao J F, Li Y Z, He G H, Li C, Zhang M W, Niu J. Effect of spraying zinc stage on grain yield and zinc content of different summer maize varieties[J]. Journal of Henan Agricultural Sciences, 2022, 51(6):22-31. doi: 10.15933/j.cnki.1004?3268.2022.06.003
[28]	张盼盼, 邵运辉, 刘京宝, 乔江方, 李川, 张美微, 赵霞, 黄璐. 施用氮锌肥对不同夏玉米品种干物质和氮锌元素累积分配的影响[J]. 华北农学报, 2022, 37(2):96-103.doi:10.7668/hbnxb.20192579. doi: 10.7668/hbnxb.20192579
	Zhang P P, Shao Y H, Liu J B, Qiao J F, Li C, Zhang M W, Zhao X, Huang L. Effect of nitrogen and zinc application on the accumulation and distribution of dry matter and nitrogen and zinc of different summer maize varieties[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2):96-103. doi: 10.7668/hbnxb.20192579
[29]	平文静. 玉米锌铁元素吸收分配的研究及相关性状的QTL定位[D]. 保定: 河北农业大学, 2019.doi:10.27109/d.cnki.ghbnu.2019.000331.
	Ping W J. Studies on absorption and distribution of zinc and iron in maize and QTL mapping of related traits[D]. Baoding: Hebei Agricultural University, 2019.
[30]	陈哲, 李盼, 刘渊, 姬东华, 赵永锋, 祝丽英, 黄亚群, 陈景堂. 玉米籽粒锌、铁含量与籽粒性状的相关及QTL定位分析[J]. 华北农学报, 2011, 26(6):6-11.doi:10.3969/j.issn.0517-6611.2009.26.060. doi: 10.7668/hbnxb.2011.06.002
	Chen Z, Li P, Liu Y, Ji D H, Zhao Y F, Zhu L Y, Huang Y Q, Chen J T. Correlation analysis and QTL mapping for kernel traits and zinc,iron content in maize[J]. Acta Agriculturae Boreali-Sinica, 2011, 26(6):6-11.
[31]	Tavajjoh M, Yasrebi J, Karimian, Olama V. Phytic acid concentration and phytic acid:zinc molar ratio in wheat cultivars and bread flours,Fars Province,Iran[J]. Journal of Agricultural Science & Technology, 2011, 13(5):743-755.doi:10.1016/j.agsy.2011.05.011. doi: 10.1016/j.agsy.2011.05.011
[32]	Von Caemmerer S, Furbank R T. Strategies for improving C4 photosynthesis[J]. Current Opinion in Plant Biology, 2016, 31:125-134.doi:10.1016/j.pbi.2016.04.003. doi: 10.1016/j.pbi.2016.04.003 pmid: 27127850
[33]	Dean Price G, Pengelly J J L, Forster B, Du J H, Whitney S M, von Caemmerer S, Badger M R, Howitt S M, Evans J R. The cyanobacterial CCM as a source of genes for improving photosynthetic CO₂ fixation in crop species[J]. Journal of Experimental Botany, 2013, 64(3):753-768.doi:10.1093/jxb/ers257. doi: 10.1093/jxb/ers257 pmid: 23028015

处理 Treatments		N含量/ (g/kg) N concentration	P含量/ (g/kg) P concentration	K含量/ (g/kg) K concentration	Ca含量/ (g/kg) Ca concentration	Mg含量/ (g/kg) Mg concentration	Cu含量/ (mg/kg) Cu concentration	Mn含量/ (mg/kg) Mn concentration	Fe含量/ (mg/kg) Fe concentration	Zn含量/ (mg/kg) Zn concentration	B含量/ (mg/kg) B concentration	Na含量/ (mg/kg) Na concentration
氮处理	N90	12.07±1.46b	8.93±1.16a	13.00±1.75a	0.24±0.04c	3.88±0.45a	2.33±0.16c	6.38±0.53a	22.02±2.12b	40.67±3.20b	43.25±1.15b	181.67±4.93a
N treatment	N180	12.77±1.27a	8.12±1.19b	12.48±1.35b	0.28±0.05b	3.70±0.38b	3.38±0.44b	5.67±0.92b	23.95±2.76b	37.46±2.13b	43.58±1.64ab	181.29±6.20a
	N225	11.85±1.21b	8.85±1.40a	12.76±1.36ab	0.32±0.08a	3.93±0.52a	4.78±0.59a	6.17±0.86ab	26.04±2.57a	46.08±2.17a	43.88±1.30a	180.88±4.25a
锌处理	Zn0	11.88±1.15b	8.72±0.87a	12.96±1.96a	0.31±0.08a	3.78±0.33a	4.00±0.30a	5.69±0.82b	25.50±2.74ab	34.22±3.34c	43.72±1.56a	183.50±6.89a
Zn treatment	Zn1	12.39±1.36ab	8.75±1.20a	12.84±1.40a	0.31±0.09a	3.88±0.45a	3.20±0.65b	7.30±0.17a	27.66±2.26a	39.33±3.88b	43.89±1.08a	182.67±3.34ab
	Zn2	12.73±1.64a	8.30±1.62b	12.34±1.43b	0.26±0.03b	3.80±0.62a	3.53±0.79ab	5.49±0.88b	22.45±2.46b	41.83±3.05b	43.00±1.33b	178.67±3.25c
	Zn3	11.92±1.16b	8.75±1.42a	12.83±1.08a	0.24±0.03b	3.90±0.42a	3.28±0.86b	5.71±0.31b	22.58±2.39b	50.22±3.91a	43.67±1.46a	180.28±5.00bc
品种	ZD958	11.28±0.73	7.62±0.66	13.96±0.10	0.27±0.05	3.49±0.49	3.58±0.60	3.01±0.16	27.70±2.73	35.39±2.20	42.61±0.80	182.14±5.68
Varieties	GSY66	13.18±1.17	9.64±0.92	11.52±0.10	0.29±0.04	4.18±0.28	3.43±0.61	9.08±0.43	21.39±1.79	47.42±4.44	44.53±1.16	180.42±4.42
N		^**	^**	^*	^**	^**	^**	ns	^**	^**	^*	ns
Zn		^**	^*	^*	^**	ns	^*	^**	^*	^**	^**	^**
V		^**	^**	^**	^*	^**	ns	^**	^**	^**	^**	ns
N×Zn		^*	^**	ns	^*	^**	^*	^**	^**	^**	^**	^**
N×V		ns	ns	^*	ns	ns	ns	ns	ns	ns	ns	ns
Zn×V		^*	^**	^**	^**	^**	^**	^*	ns	^**	^*	^**
N×Zn×V		ns	^**	^**	ns	^**	^*	^**	ns	^**	^*	ns

处理 Treatments		N含量/ (g/kg) N concentration	P含量/ (g/kg) P concentration	K含量/ (g/kg) K concentration	Ca含量/ (g/kg) Ca concentration	Mg含量/ (g/kg) Mg concentration	Cu含量/ (mg/kg) Cu concentration	Mn含量/ (mg/kg) Mn concentration	Fe含量/ (mg/kg) Fe concentration	Zn含量/ (mg/kg) Zn concentration	B含量/ (mg/kg) B concentration	Na含量/ (mg/kg) Na concentration
氮处理	N90	12.07±1.46b	8.93±1.16a	13.00±1.75a	0.24±0.04c	3.88±0.45a	2.33±0.16c	6.38±0.53a	22.02±2.12b	40.67±3.20b	43.25±1.15b	181.67±4.93a
N treatment	N180	12.77±1.27a	8.12±1.19b	12.48±1.35b	0.28±0.05b	3.70±0.38b	3.38±0.44b	5.67±0.92b	23.95±2.76b	37.46±2.13b	43.58±1.64ab	181.29±6.20a
	N225	11.85±1.21b	8.85±1.40a	12.76±1.36ab	0.32±0.08a	3.93±0.52a	4.78±0.59a	6.17±0.86ab	26.04±2.57a	46.08±2.17a	43.88±1.30a	180.88±4.25a
锌处理	Zn0	11.88±1.15b	8.72±0.87a	12.96±1.96a	0.31±0.08a	3.78±0.33a	4.00±0.30a	5.69±0.82b	25.50±2.74ab	34.22±3.34c	43.72±1.56a	183.50±6.89a
Zn treatment	Zn1	12.39±1.36ab	8.75±1.20a	12.84±1.40a	0.31±0.09a	3.88±0.45a	3.20±0.65b	7.30±0.17a	27.66±2.26a	39.33±3.88b	43.89±1.08a	182.67±3.34ab
	Zn2	12.73±1.64a	8.30±1.62b	12.34±1.43b	0.26±0.03b	3.80±0.62a	3.53±0.79ab	5.49±0.88b	22.45±2.46b	41.83±3.05b	43.00±1.33b	178.67±3.25c
	Zn3	11.92±1.16b	8.75±1.42a	12.83±1.08a	0.24±0.03b	3.90±0.42a	3.28±0.86b	5.71±0.31b	22.58±2.39b	50.22±3.91a	43.67±1.46a	180.28±5.00bc
品种	ZD958	11.28±0.73	7.62±0.66	13.96±0.10	0.27±0.05	3.49±0.49	3.58±0.60	3.01±0.16	27.70±2.73	35.39±2.20	42.61±0.80	182.14±5.68
Varieties	GSY66	13.18±1.17	9.64±0.92	11.52±0.10	0.29±0.04	4.18±0.28	3.43±0.61	9.08±0.43	21.39±1.79	47.42±4.44	44.53±1.16	180.42±4.42
N		^**	^**	^*	^**	^**	^**	ns	^**	^**	^*	ns
Zn		^**	^*	^*	^**	ns	^*	^**	^*	^**	^**	^**
V		^**	^**	^**	^*	^**	ns	^**	^**	^**	^**	ns
N×Zn		^*	^**	ns	^*	^**	^*	^**	^**	^**	^**	^**
N×V		ns	ns	^*	ns	ns	ns	ns	ns	ns	ns	ns
Zn×V		^*	^**	^**	^**	^**	^**	^*	ns	^**	^*	^**
N×Zn×V		ns	^**	^**	ns	^**	^*	^**	ns	^**	^*	ns

氮处理 N treatments		锌处理 Zn treatments		品种 Varieties		N含量 N concentration		P含量 P concentration		K含量 K concentration		Ca含量 Ca concentration		Mg含量 Mg concentration
N90		Zn0		ZD958		10.79±0.35gh		8.21±0.25def		15.82±0.72a		0.23±0.03g		3.48±0.08fgh
				GSY66		12.10±0.24defgh		8.76±0.03cd		10.78±0.01jk		0.31±0.03cde		3.75±0.04def
		Zn1		ZD958		11.27±0.39fgh		7.72±0.11efg		13.63±0.02bcde		0.25±0.00efg		3.54±0.14efgh
				GSY66		12.93±0.60bcde		9.91±0.03ab		11.85±0.23ghij		0.24±0.02efg		4.35±0.12ab
		Zn2		ZD958		10.66±0.67h		7.76±0.14efg		14.35±0.16bc		0.22±0.01g		3.45±0.03fgh
				GSY66		14.22±0.24ab		10.69±0.30a		11.63±0.27hij		0.23±0.02efg		4.67±0.13a
		Zn3		ZD958		10.99±0.19gh		8.12±0.11def		14.3±0.03bcd		0.23±0.02fg		3.64±0.02efg
				GSY66		13.57±0.67abc		10.29±0.05ab		11.61±0.24hij		0.23±0.01g		4.13±0.03bcd
N180		Zn0		ZD958		11.26±0.04fgh		7.48±0.09fgh		13.36±0.47cdef		0.26±0.00efg		3.41±0.04fgh
				GSY66		12.73±0.50cdef		9.86±0.11ab		12.09±0.48ghi		0.38±0.05abc		4.29±0.09abc
		Zn1		ZD958		12.24±0.05cdefg		7.72±0.29efg		14.14±0.52bcd		0.31±0.01cdef		3.52±0.01fgh
				GSY66		14.63±0.17a		8.67±0.08cde		11.28±0.19hijk		0.30±0.03defg		3.80±0.04def
	Zn2		ZD958		12.12±0.37defgh		6.65±0.11h		13.38±0.38cdef		0.26±0.00efg		3.25±0.03gh
			GSY66		14.64±0.23a		8.15±0.09def		10.34±0.31k		0.27±0.00efg		3.71±0.08ef
	Zn3		ZD958		11.60±0.19efgh		6.89±0.04gh		13.21±0.36def		0.22±0.01g		3.52±0.08fgh
			GSY66		12.97±0.16bcde		9.48±0.79bc		12.02±0.44ghi		0.27±0.01efg		4.14±0.29bcd
N225	Zn0		ZD958		11.54±0.73efgh		8.53±0.22cde		14.58±0.01b		0.29±0.00defg		3.80±0.02def
			GSY66		12.87±1.04bcde		9.47±0.40bc		11.15±0.22ijk		0.42±0.02ab		3.95±0.17cde
	Zn1		ZD958		10.80±0.21gh		7.88±0.24defg		14.58±0.08b		0.43±0.03a		3.55±0.09efgh
			GSY66		12.48±0.32cdef		10.62±0.20a		11.57±0.00hij		0.35±0.08bcd		4.51±0.20ab
	Zn2		ZD958		11.28±0.11fgh		6.71±0.04h		12.74±0.09efg		0.29±0.00defg		3.21±0.05h
			GSY66		13.43±0.58abcd		9.82±0.72ab		11.63±0.52hij		0.27±0.01efg		4.5±0.23ab
	Zn3		ZD958		10.82±0.27gh		7.75±0.53efg		13.48±0.28bcde		0.26±0.02efg		3.54±0.20efgh
			GSY66		11.60±0.39efgh		9.98±0.38ab		12.36±0.57fgh		0.25±0.01efg		4.41±0.12ab

氮处理 N treatments		锌处理 Zn treatments		品种 Varieties		N含量 N concentration		P含量 P concentration		K含量 K concentration		Ca含量 Ca concentration		Mg含量 Mg concentration
N90		Zn0		ZD958		10.79±0.35gh		8.21±0.25def		15.82±0.72a		0.23±0.03g		3.48±0.08fgh
				GSY66		12.10±0.24defgh		8.76±0.03cd		10.78±0.01jk		0.31±0.03cde		3.75±0.04def
		Zn1		ZD958		11.27±0.39fgh		7.72±0.11efg		13.63±0.02bcde		0.25±0.00efg		3.54±0.14efgh
				GSY66		12.93±0.60bcde		9.91±0.03ab		11.85±0.23ghij		0.24±0.02efg		4.35±0.12ab
		Zn2		ZD958		10.66±0.67h		7.76±0.14efg		14.35±0.16bc		0.22±0.01g		3.45±0.03fgh
				GSY66		14.22±0.24ab		10.69±0.30a		11.63±0.27hij		0.23±0.02efg		4.67±0.13a
		Zn3		ZD958		10.99±0.19gh		8.12±0.11def		14.3±0.03bcd		0.23±0.02fg		3.64±0.02efg
				GSY66		13.57±0.67abc		10.29±0.05ab		11.61±0.24hij		0.23±0.01g		4.13±0.03bcd
N180		Zn0		ZD958		11.26±0.04fgh		7.48±0.09fgh		13.36±0.47cdef		0.26±0.00efg		3.41±0.04fgh
				GSY66		12.73±0.50cdef		9.86±0.11ab		12.09±0.48ghi		0.38±0.05abc		4.29±0.09abc
		Zn1		ZD958		12.24±0.05cdefg		7.72±0.29efg		14.14±0.52bcd		0.31±0.01cdef		3.52±0.01fgh
				GSY66		14.63±0.17a		8.67±0.08cde		11.28±0.19hijk		0.30±0.03defg		3.80±0.04def
	Zn2		ZD958		12.12±0.37defgh		6.65±0.11h		13.38±0.38cdef		0.26±0.00efg		3.25±0.03gh
			GSY66		14.64±0.23a		8.15±0.09def		10.34±0.31k		0.27±0.00efg		3.71±0.08ef
	Zn3		ZD958		11.60±0.19efgh		6.89±0.04gh		13.21±0.36def		0.22±0.01g		3.52±0.08fgh
			GSY66		12.97±0.16bcde		9.48±0.79bc		12.02±0.44ghi		0.27±0.01efg		4.14±0.29bcd
N225	Zn0		ZD958		11.54±0.73efgh		8.53±0.22cde		14.58±0.01b		0.29±0.00defg		3.80±0.02def
			GSY66		12.87±1.04bcde		9.47±0.40bc		11.15±0.22ijk		0.42±0.02ab		3.95±0.17cde
	Zn1		ZD958		10.80±0.21gh		7.88±0.24defg		14.58±0.08b		0.43±0.03a		3.55±0.09efgh
			GSY66		12.48±0.32cdef		10.62±0.20a		11.57±0.00hij		0.35±0.08bcd		4.51±0.20ab
	Zn2		ZD958		11.28±0.11fgh		6.71±0.04h		12.74±0.09efg		0.29±0.00defg		3.21±0.05h
			GSY66		13.43±0.58abcd		9.82±0.72ab		11.63±0.52hij		0.27±0.01efg		4.5±0.23ab
	Zn3		ZD958		10.82±0.27gh		7.75±0.53efg		13.48±0.28bcde		0.26±0.02efg		3.54±0.20efgh
			GSY66		11.60±0.39efgh		9.98±0.38ab		12.36±0.57fgh		0.25±0.01efg		4.41±0.12ab

氮处理 N treatments	锌处理 Zn treatments	品种 Varieties	Cu含量 Cu concentration	Mn含量 Mn concentration	Fe含量 Fe concentration	Zn含量 Zn concentration	B含量 B concentration	Na含量 Na concentration
N90	Zn0	ZD958	2.13±0.25defg	2.92±1.03hij	18.15±0.77d	25.81±1.17g	42.21±0.11hi	183.49±3.57bc
		GSY66	2.89±0.63def	6.82±0.68ef	28.97±6.05cd	27.31±1.73g	44.55±0.51abcd	178.33±0.08cd
	Zn1	ZD958	1.02±0.42g	1.67±0.84ij	18.79±0.01d	27.84±1.29g	42.16±0.33ghi	182.85±1.00bc
		GSY66	2.34±0.09efg	12.32±0.24ab	21.00±1.2cd	44.63±0.77cd	43.70±0.24cdefg	181.03±3.01bcd
	Zn2	ZD958	2.19±0.93defg	1.96±0.09ij	17.77±0.93d	35.02±1.22defg	42.45±0.28ghi	180.03±2.39bcd
		GSY66	3.36±0.25cde	13.42±0.9a	24.68±0.62cd	59.95±0.58a	43.46±0.08defgh	181.24±1.71bcd
	Zn3	ZD958	1.67±0.05efg	3.10±0.34hi	24.30±2.56cd	47.71±2.50bc	43.10±0.18efghi	180.02±1.22bcd
		GSY66	2.54±0.32defg	8.47±0.18de	22.50±1.00cd	57.04±1.89ab	44.87±0.83abcd	186.67±5.78b
N180	Zn0	ZD958	3.67±0.26bcd	0.68±0.09j	21.89±0.55cd	26.48±2.41g	42.39±0.19ghi	194.49±1.06a
		GSY66	5.14±0.22ab	11.01±1.07bc	35.05±9.20bc	44.73±4.92cd	45.21±0.18ab	180.68±1.6bcd
	Zn1	ZD958	4.26±0.07bcd	4.60±0.64gh	21.95±0.26cd	31.20±2.65efg	43.22±0.38defgh	179.79±2.15bcd
		GSY66	3.40±0.24cde	8.50±0.10de	23.32±2.35cd	41.69±0.02cde	45.50±0.04ab	185.09±1.89bc
	Zn2	ZD958	1.40±0.5fg	1.13±0.08j	24.54±0.52cd	29.05±0.09fg	41.89±0.34i	175.36±0.29d
		GSY66	5.01±0.08abc	5.72±0.08fg	20.34±0.45d	39.99±3.58cdef	43.25±0.52efghi	178.17±2.61cd
	Zn3	ZD958	2.15±0.58efg	3.17±1.12hij	23.52±1.11cd	35.48±4.54defg	41.93±0.24hi	178.82±1.96cd
		GSY66	2.21±0.27defg	9.87±1.01cd	20.96±2.28cd	50.9±8.82abc	45.85±0.37a	177.75±1.11cd
N225	Zn0	ZD958	5.10±0.05abc	3.47±0.37hi	21.47±0.36cd	45.41±0.27cd	43.17±0.17efghi	187.38±3.99b
		GSY66	5.13±0.63abc	9.17±0.56cde	24.51±0.92cd	36.08±1.51defg	44.75±1.02abc	177.66±0.21cd
	Zn1	ZD958	5.43±0.90ab	4.53±0.36gh	40.10±3.41a	39.56±1.70cdef	44.10±0.29abcde	185.31±1.65bc
		GSY66	3.43±0.87cde	12.07±0.11ab	36.01±16.04ab	49.86±6.50abc	44.17±0.30abcde	182.42±0.01bcd
	Zn2	ZD958	2.87±0.55def	1.07±0.13j	23.80±3.00cd	30.45±3.34fg	42.45±0.08ghi	179.14±0.99cd
		GSY66	6.00±0.58a	9.50±0.94cd	20.81±0.69cd	56.74±3.22ab	45.49±0.27ab	178.09±2.05cd
	Zn3	ZD958	3.90±1.18bcd	1.83±0.00ij	20.71±2.24cd	50.12±6.10abc	42.59±0.42fghi	180.15±0.44bcd
		GSY66	6.37±0.81a	7.50±0.74ef	20.91±1.79cd	60.28±3.40a	44.37±0.62bcdef	178.06±0.96cd

选择文件类型/文献管理软件名称

选择包含的内容