蓖麻单穗籽粒产量和穗轴干质量QTL定位分析

doi:10.7668/hbnxb.20195009

摘要/Abstract

摘要：

蓖麻单穗籽粒产量和穗轴干质量对单株产量有重要影响。为揭示蓖麻单穗籽粒产量和穗轴干质量的遗传基础,利用目标性状有显著差异的杂交组合(9048×16-201)构建了F₂和BC₁(F₁×P₂)群体,采用复合区间作图法(CIM)和完备区间作图法(ICIM-ADD)对主穗和一级分枝穗籽粒产量、穗轴干质量等4个性状进行了QTL定位分析。在F₂群体中共检测到7/10(CIM/ICIM-ADD)个QTL,其中,主穗籽粒产量、主穗穗轴干质量和一级分枝穗穗轴干质量QTL分别有3/6,3/3,1/1个,总贡献率分别为15.81%/24.09%,2.84%/8.65%,6.49%/6.56%。在BC₁群体中共检测到1/3个QTL,主穗籽粒产量和一级分枝穗穗轴干质量分别有0/2,1/1个QTL,总贡献率分别为0/10.28%,11.60%/10.22%;在检测到的所有QTL中,qPSSY3.1和qPBSADW3.1是稳定QTL,后者同时也是主效QTL,贡献率分别为3.76%~7.00%和6.49%~11.60%。它们重叠分布在RCM920~RCM950标记区间内,共同构成一个QTL簇(QTL-cluster4);还检测到数十对上位性QTL,所有性状的上位性效应占表型总贡献率的比例均在19%以上,表明上位性效应是重要的遗传组分。上述结果为理解蓖麻单穗籽粒产量和穗轴干质量的遗传结构提供了参考,并为其标记辅助选择提供了可用的分子标记。

关键词: 蓖麻, 单穗籽粒产量, 穗轴干质量, QTL定位, 主效QTL

Abstract:

The seed yield per spike and spike axis dry weight affect yield per plant.In order to reveal the genetic basis,QTLs were mapped with populations F₂ and BC₁ (F₁×P₂) derived from the cross combination 9048×16-201 using CIM and ICIM-ADD methods.A total of 7/10 (CIM/ICIM-ADD) QTLs were detected in the F₂ population,including 3/6,3/3 and 1/1 QTLs conferring PSSY,PSADW and PBSADW respectively,with a contribution rate of 15.81%/24.09%,2.84%/8.65% and 6.49%/6.56%,respectively.And 1/3 QTLs were identified in the BC₁ population,including 0/2 and 1/1 QTLs underlying PSSY and PBSADW,with a contribution rate of 0/10.28% and 11.60%/10.22% respectively.Among all the detected QTLs,qPSSY3.1 and qPBSADW3.1 were stable QTLs,and the latter was also the main-effect QTL,with contribution rates ranging from 3.76%—7.00% and 6.49%—11.60%,respectively.They overlapped in the marker interval RCM920—RCM950,forming a QTL cluster (i.e.,QTL-cluster4).Additionally,a dozen pairs of epistatic QTLs were detected with the epistatic effect of these traits accounting for more than 19% of the total contribution rate,indicating that the epistatic effect was an important genetic component of seed yield per spike and spike axis dry weight in castor.The results not only provided a reference for the understanding of the genetic structure of seed yield per spike and spike axis dry weight but also offered available molecular markers for marker-assisted selection.

Key words: Castor, Seed yield per spike, Spike axis dry weight, QTL mapping, Main-effect QTL

林海虹, 陆建农, 殷学贵, 黄冠荣, 张柳琴, 张肖肖, 刘朝裕, 左金鹰. 蓖麻单穗籽粒产量和穗轴干质量QTL定位分析[J]. 华北农学报, 2024, 39(6): 94-105. doi: 10.7668/hbnxb.20195009.

LIN Haihong, LU Jiannong, YIN Xuegui, HUANG Guanrong, ZHANG Liuqin, ZHANG Xiaoxiao, LIU Chaoyu, ZUO Jinying. Mapping QTLs Conferring Seed Yield Per Spike and Spike Axis Dry Weight in Castor[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(6): 94-105. doi: 10.7668/hbnxb.20195009.

http://www.hbnxb.net/CN/Y2024/V39/I6/94

图/表 9

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群体 Population	性状 Trait	相关系数 Correlation coefficient	通径系数 Path coefficient				决策系数 Decision coefficient
群体 Population	性状 Trait	相关系数 Correlation coefficient	PSADW	PSPDW	PSHGW	PSSN	决策系数 Decision coefficient
F₂	PSADW	0.399	0.070	—	0.037	0.292	0.329	0.050 960
	PSPDW	—	—	—	—	—	—	—
	PSHGW	-0.020	0.006	—	0.451	-0.477	-0.472	-0.221 441
	PSSN	0.870	0.019	—	-0.204	1.054	-0.185	0.723 044
BC₁	PSADW	0.628	0.161	-0.000 05	0.148	0.128	0.276	0.176 295
	PSPDW	-0.072	0.008	-0.001 00	0.145	-0.090	0.063	0.000 143
	PSHGW	0.246	0.055	-0.000 33	0.434	-0.097	-0.043	0.025 172
	PSSN	0.832	0.058	0.000 25	-0.118	0.358	-0.060	0.688 624

群体 Population	性状 Trait	相关系数 Correlation coefficient	通径系数 Path coefficient				决策系数 Decision coefficient
群体 Population	性状 Trait	相关系数 Correlation coefficient	PSADW	PSPDW	PSHGW	PSSN	决策系数 Decision coefficient
F₂	PSADW	0.399	0.070	—	0.037	0.292	0.329	0.050 960
	PSPDW	—	—	—	—	—	—	—
	PSHGW	-0.020	0.006	—	0.451	-0.477	-0.472	-0.221 441
	PSSN	0.870	0.019	—	-0.204	1.054	-0.185	0.723 044
BC₁	PSADW	0.628	0.161	-0.000 05	0.148	0.128	0.276	0.176 295
	PSPDW	-0.072	0.008	-0.001 00	0.145	-0.090	0.063	0.000 143
	PSHGW	0.246	0.055	-0.000 33	0.434	-0.097	-0.043	0.025 172
	PSSN	0.832	0.058	0.000 25	-0.118	0.358	-0.060	0.688 624

性状 Trait	世代 Generation	群体规模/株 Population size	极差 Range	均值±标准差 Mean±s	变异系数/% CV	偏度 Skewness	峰度 Kurtosis
主穗籽粒产量	P₁	25	84.20~108.57	92.61±7.73A	8.17	-0.02	-1.93
Seed yield of	P₂	25	11.14~16.67	13.28±1.48B	11.11	0.93	0.97
primary spike	F₁	25	30.74~39.22	34.75±2.11	6.07	0.08	1.82
	F₂	282	1.83~121.50	41.81±21.17	50.63	0.92	0.81
	BC₁	250	2.34~165.89	33.29±19.86	59.64	2.35	10.88
一级分枝穗籽粒产量	P₁	25	30.22~42.50	37.59±3.68A	9.80	-0.75	0.00
Seed yield of primary	P₂	25	4.21~9.86	6.89±1.66B	24.15	0.34	-0.11
branch spike	F₁	25	10.04~16.96	13.18±2.32	17.61	-0.06	-1.26
	F₂	282	1.74~255.95	67.38±41.80	62.04	0.87	1.04
	BC₁	250	0.30~217.89	93.01±43.00	46.24	0.17	-0.07
主穗穗轴干质量	P₁	25	10.00~15.00	13.18±2.52A	19.14	-0.66	-1.96
Dry weight of primary	P₂	25	5.00~5.00	5.00±0.00B	0.01
spike axis	F₁	25	5.00~10.00	8.33±2.46	29.54	-0.81	-1.65
	F₂	282	5.00~55.00	7.22±6.19	85.80	5.77	39.24
	BC₁	250	5.00~25.00	5.91±2.66	44.96	4.03	21.17
一级分枝穗穗轴干质量	P₁	25	7.50~11.67	8.86±1.84A	20.71	1.05	-0.90
Dry weight of primary	P₂	25	1.25~2.00	1.70±0.29B	16.86	-0.49	-0.89
branch spike axis	F₁	25	3.33~4.17	3.88±0.30	7.85	-0.93	-0.21
	F₂	282	0.56~12.00	2.58±1.64	63.50	1.92	5.73
	BC₁	250	0.63~5.00	1.93±0.89	45.92	0.99	0.94

性状 Trait	世代 Generation	群体规模/株 Population size	极差 Range	均值±标准差 Mean±s	变异系数/% CV	偏度 Skewness	峰度 Kurtosis
主穗籽粒产量	P₁	25	84.20~108.57	92.61±7.73A	8.17	-0.02	-1.93
Seed yield of	P₂	25	11.14~16.67	13.28±1.48B	11.11	0.93	0.97
primary spike	F₁	25	30.74~39.22	34.75±2.11	6.07	0.08	1.82
	F₂	282	1.83~121.50	41.81±21.17	50.63	0.92	0.81
	BC₁	250	2.34~165.89	33.29±19.86	59.64	2.35	10.88
一级分枝穗籽粒产量	P₁	25	30.22~42.50	37.59±3.68A	9.80	-0.75	0.00
Seed yield of primary	P₂	25	4.21~9.86	6.89±1.66B	24.15	0.34	-0.11
branch spike	F₁	25	10.04~16.96	13.18±2.32	17.61	-0.06	-1.26
	F₂	282	1.74~255.95	67.38±41.80	62.04	0.87	1.04
	BC₁	250	0.30~217.89	93.01±43.00	46.24	0.17	-0.07
主穗穗轴干质量	P₁	25	10.00~15.00	13.18±2.52A	19.14	-0.66	-1.96
Dry weight of primary	P₂	25	5.00~5.00	5.00±0.00B	0.01
spike axis	F₁	25	5.00~10.00	8.33±2.46	29.54	-0.81	-1.65
	F₂	282	5.00~55.00	7.22±6.19	85.80	5.77	39.24
	BC₁	250	5.00~25.00	5.91±2.66	44.96	4.03	21.17
一级分枝穗穗轴干质量	P₁	25	7.50~11.67	8.86±1.84A	20.71	1.05	-0.90
Dry weight of primary	P₂	25	1.25~2.00	1.70±0.29B	16.86	-0.49	-0.89
branch spike axis	F₁	25	3.33~4.17	3.88±0.30	7.85	-0.93	-0.21
	F₂	282	0.56~12.00	2.58±1.64	63.50	1.92	5.73
	BC₁	250	0.63~5.00	1.93±0.89	45.92	0.99	0.94

群体 Population	方法 Method	性状 Trait	QTL	染色体 Chromosome	连锁群 LG	位置 Position	LOD	加性 Add	显性 Dom	贡献率/% PVE	置信区间 CI	标记区间 MI
F₂	CIM	PSSY	FqPSSY9.1	9	3	8.01	37.00	75.00	-3.06	6.29	1.7~38.0	RCM820~RCM523
			FqPSSY10.1	10	4	22.61	3.21	6.76	-3.75	2.52	4.5~27.6	RCM938~RCM279
			qPSSY3.1	3	10	71.11	3.34	8.87	-0.52	7.00	62.0~80.1	RCM931~RCM922
		PSADW	FqPSADW7.1	7	1	2.01	40.47	-23.02	-22.20	1.74	1.0~2.4	RCM1335~RCM1336
			FqPSADW10.1	10	4	8.01	44.96	22.11	-22.86	0.01	5.5~11.1	RCM938~RCM872
			FqPSADW10.2	10	4	73.91	3.01	-4.39	-3.40	1.09	72.2~80.3	RCM226~RCM945
		PBSADW	qPBSADW3.1	3	10	75.11	2.01	0.24	-0.83	6.49	47.6~80.1	RCM933~RCM922
	ICIM-ADD	PSSY	FqPSSY7.1	7	1	18.00	5.28	-8.76	-1.18	5.71	16.5~18.0	RCM1336~RCM1338
			FqPSSY9.1	9	3	29.00	4.00	8.96	-2.28	5.36	25.5~37.5	RCM1212~RCM523
			FqPSSY9.2	9	3	81.00	2.01	1.61	7.02	2.00	76.5~92.5	RCM1582~RCM67
			FqPSSY10.1	10	4	32.00	2.66	-4.57	-6.52	3.08	18.5~53.5	RCM279~RCM1567
			FqPSSY10.2	10	4	79.00	3.04	-5.84	-7.23	4.18	70.5~87.0	RCM226~RCM945
			qPSSY3.1	3	10	70.00	2.62	7.81	-2.67	3.76	52.5~81.0	RCM931~RCM922
		PSADW	FqPSADW7.1	7	1	3.00	3.20	-3.19	-2.41	0.93	0.0~15.5	RCM1335~RCM1336
			FqPSADW10.1	10	4	9.00	46.19	2.44	-22.96	7.25	5.5~11.5	RCM938~RCM872
			FqPSADW2.1	2	8	1.00	2.23	1.60	-1.51	0.47	0.0~15.0	RCM150~RCM1824
		PBSADW	qPBSADW3.1	3	10	81.00	2.38	0.33	-0.66	6.56	66.5~81.0	RCM931~RCM922
BC₁	CIM	PBSADW	qPBSADW3.1	3	4	14.41	5.33	0.61		11.60	10.8~17.8	RCM950~RCM920
	ICIM-ADD	PSSY	BqPSSY4.1	4	3	23.00	2.27	9.29		4.85	0.0~43.5	RCM904~RCM523
			qPSSY3.1	3	4	1.00	2.79	9.61		5.43	33.5~53.0	RCM931~RCM922
		PBSADW	qPBSADW3.1	3	4	4.00	5.40	0.63		10.22	10.5~17.5	RCM950~RCM920

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