芝麻芽期耐铝胁迫相关性状的QTL鉴定

doi:10.7668/hbnxb.20193137

摘要/Abstract

摘要：

为解析芝麻耐铝毒遗传机制,发掘和定位芝麻耐铝毒QTL,采用不同浓度铝离子对地方品种金黄麻和竹山白进行了芽期胁迫处理,确定芽期铝胁迫处理适宜浓度。利用耐铝毒芝麻种质金黄麻和铝敏感芝麻种质竹山白为亲本构建的一个含有180个家系的重组自交系群体,以铝胁迫下芝麻芽期相对根长(RRL)、相对芽长(RSL)和相对苗鲜质量(RSW)作为表型指标,结合通过重测序构建的一个含有1 354个bin标记的高密度遗传图谱,采用复合区间作图法,对芝麻芽期耐铝毒性状进行了QTL定位和候选基因筛选。3个性状在群体中呈显著正相关,共检测到7个QTL,分布于6条染色体上(2,4,6,10,11,13号染色体),其中3个与相对根长相关,2个与相对芽长相关,2个与相对苗鲜质量有关。其中位于2号染色体的2个分别控制相对根长和相对苗鲜质量的QTL区间部分重叠。候选基因分析的结果表明,15个预测基因可能与耐铝胁迫相关,主要参与金属转运、脱毒和金属离子的跨膜结合、GDSL基序酯酶、过氧化物酶体以及生物体内有毒物质的代谢与解毒等生理进程。

关键词: 芝麻, 铝胁迫, QTL定位, 候选基因

Abstract:

To elucidate the genetic mechanism and identify quantitative trait loci(QTL)for Aluminum(Al)tolerance in sesame.We investigated the response of Al stress using sesame landraces Jinhuangma(JHM)and Zhushanbai(ZSB)with different concentrations of Al³⁺ and confirmed a suitable Al³⁺ concentration for stress.Three traits including relative root length(RRL),relative shoot length(RSL)and relative fresh seedling weight(RSW)were evaluated in a recombinant inbred line(RIL)population with 180 lines that derived from two parents that contrasts for Al tolerance(ZSB and JHM).Quantitative trait loci(QTL)mapping were conducted by using a high resolution genetic map containing 1 354 bin markers through composition interval mapping(CIM)method.Significant positive correlations were observed among the three traits.A total of 7 QTLs were detected on 6 chromosomes(Chr2,Chr4,Chr6,Chr10,Chr11 and Chr13),including 3 RRL related QTLs,2 RSL related QTLs and 2 RSW associated QTLs.Among them,two QTLs associated with RRL and RSW were mapped to the overlapped interval on chromosome 2.Putative candidate gene analysis revealed that 15 genes may be related to Al tolerance.These genes mainly involved in metal transport and detoxification,transmembrane binding of metal ions,GDSL-motif lipase,peroxisomal catalase and metabolism and detoxification of toxic substances in organisms.

Key words: Sesame, Al stress, QTL mapping, Candidate gene

梁俊超, 赵云燕, 王郅琪, 孙建, 颜廷献, 颜小文, 饶月亮, 周红英, 乐美旺. 芝麻芽期耐铝胁迫相关性状的QTL鉴定[J]. 华北农学报, 2022, 37(6): 124-131. doi: 10.7668/hbnxb.20193137.

LIANG Junchao, ZHAO Yunyan, WANG Zhiqi, SUN Jian, YAN Tingxian, YAN Xiaowen, RAO Yueliang, ZHOU Hongying, LE Meiwang. Identification of QTL for Aluminum Tolerance Related Traits at the Early Seedling Stage in Sesame[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(6): 124-131. doi: 10.7668/hbnxb.20193137.

http://www.hbnxb.net/CN/Y2022/V37/I6/124

图/表 6

参考文献 39

[1]	Wang J P, Raman H, Zhang G P, Mendham N, Zhou M X. Aluminium tolerance in barley(Hordeum vulgare L.): Physiological mechanisms,genetics and screening methods[J]. Journal of Zhejiang University Science (Life Science), 2006, 7(10): 769-787.
[2]	Sade H, Meriga B, Surapu V, Gadi J, Sunita M S L, Suravajhala P, Kishor P B K. Toxicity and tolerance of aluminum in plants: Tailoring plants to suit to acid soils[J]. BioMetals, 2016, 29(2): 187-210.doi:10.1007/s10534-016-9910-z. doi: 10.1007/s10534-016-9910-z pmid: 26796895
[3]	肖厚军, 王正银. 酸性土壤铝毒与植物营养研究进展[J]. 西南农业学报, 2006, 19(6): 1180-1188.doi:10.16213/j.cnki.scjas.2006.06.045. doi: 10.16213/j.cnki.scjas.2006.06.045
	Xiao H J, Wang Z Y. Advance on study of aluminum toxicity and plant nutrition in acid soils[J]. Southwest China Journal of Agricultural Sciences, 2006, 19(6): 1180-1188.
[4]	Magalhaes J V, Liu J P, Guimarães C T, Lana U G P, Alves V M C, Wang Y H, Schaffert R E, Hoekenga O A, Piñeros M A, Shaff J E, Klein P E, Carneiro N P, Coelho C M, Trick H N, Kochian L V. A gene in the multidrug and toxic compound extrusion(MATE)family confers aluminum tolerance in Sorghum[J]. Nature Genetics, 2007, 39(9): 1156-1161.doi:10.1038/ng2074. doi: 10.1038/ng2074 pmid: 17721535
[5]	Tang Y, Sorrells M E, Kochian L V, Garvin D F. Identification of RFLP markers linked to the barley aluminum tolerance gene Alp[J]. Crop Science, 2000, 40(3):778-782.doi: 10.2135/cropsci2000.403778x. doi: 10.2135/cropsci2000.403778x URL
[6]	Navakode S, Weidner A, Varshney R, Lohwasser U, Scholz U, Börner A. A QTL analysis of aluminium tolerance in barley,using gene-based markers[J]. Cereal Research Communications, 2009, 37(4): 531-540.doi: 10.1556/crc.37.2009.4.6. doi: 10.1556/crc.37.2009.4.6 URL
[7]	Xue Y, Jiang L, Su N, Wang J K, Deng P, Ma J F, Zhai H Q, Wan J M. The genetic basic and fine-mapping of a stable quantitative-trait loci for aluminium tolerance in rice[J]. Planta, 2007, 227(1): 255-262.doi:10.1007/s00425-007-0613-0. doi: 10.1007/s00425-007-0613-0 pmid: 17721709
[8]	Famoso A N, Zhao K Y, Clark R T, Tung C W, Wright M H, Bustamante C, Kochian L V, McCouch S R. Genetic architecture of aluminum tolerance in rice(Oryza sativa)determined through genome-wide association analysis and QTL mapping[J]. PLoS Genetics, 2011, 7(8): e1002221.doi:10.1371/journal.pgen.1002221. doi: 10.1371/journal.pgen.1002221 URL
[9]	Meng L J, Wang B X, Zhao X Q, Ponce K, Qian Q, Ye G Y. Association mapping of ferrous,zinc,and aluminum tolerance at the seedling stage in Indica rice using MAGIC populations[J]. Frontiers in Plant Science, 2017, 8: 1822.doi:10.3389/fpls.2017.01822. doi: 10.3389/fpls.2017.01822 URL
[10]	胡萃, 刘强, 龙婉婉, 李芙蓉, 李荣刚. 铝胁迫对芝麻种子萌发和根系生长的影响[J]. 江苏农业科学, 2009, 37(3): 60-62.doi:10.3969/j.issn.1002-1302.2009.03.023. doi: 10.3969/j.issn.1002-1302.2009.03.023
	Hu C, Liu Q, Long W W, Li F R, Li R G. Effects of aluminum on the seed germinating and root growth in sesame(Sesamum indicum L.)[J]. Jiangsu Agricultural Sciences, 2009, 37(3): 60-62.
[11]	贺根和, 刘强, 邓鹏, 吴杨. 铝胁迫对芝麻根系分泌物的影响[J]. 江苏农业科学, 2010, 38(5): 117-119.doi:10.15889/j.issn.1002-1302.2010.05.065. doi: 10.15889/j.issn.1002-1302.2010.05.065
	He G H, Liu Q, Deng P, Wu Y. Effects of aluminum stress on root exudates in sesame[J]. Jiangsu Agricultural Sciences, 2010, 38(5): 117-119.
[12]	孙建, 乐美旺, 饶月亮, 颜廷献, 颜小文, 周红英. 芽期Al³⁺胁迫对芝麻幼苗生长的影响及种质资源耐铝毒性评价[J]. 中国油料作物学报, 2014, 36(5): 602-609.doi:10.7505/j.issn.1007-9084.2014.05.007. doi: 10.7505/j.issn.1007-9084.2014.05.007
	Sun J, Le M W, Rao Y L, Yan T X, Yan X W, Zhou H Y. Effect of aluminum stress on sesame seedling growth during germination and tolerance evaluation of sesame germplasm[J]. Chinese Journal of Oil Crop Sciences, 2014, 36(5): 602-609.
[13]	Liang J C, Sun J, Ye Y Y, Yan X W, Yan T X, Rao Y L, Zhou H Y, Le M W. QTL mapping of PEG-induced drought tolerance at the early seedling stage in sesame using whole genome re-sequencing[J]. PLoS One, 2021, 16(2): e0247681.doi:10.1371/journal.pone.0247681. doi: 10.1371/journal.pone.0247681 URL
[14]	Wang S, Basten C J, Zeng Z B. Windows QTL cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh,NC[EB/OL]. [2012—02—05]. https://brcwebportal.cos.ncsu.edu/qtlcart/WQTLCart.htm. URL
[15]	Wang L H, Xia Q J, Zhang Y X, Zhu X D, Zhu X F, Li D H, Ni X M, Gao Y, Xiang H T, Wei X, Yu J Y, Quan Z W, Zhang X R. Updated sesame genome assembly and fine mapping of plant height and seed coat color QTLs using a new high-density genetic map[J]. BMC Genomics, 2016, 17: 31.doi:10.1186/s12864-015-2316-4. doi: 10.1186/s12864-015-2316-4 pmid: 26732604
[16]	Halimaa P, Lin Y F, Ahonen V H, Blande D, Clemens S, Gyenesei A, Häikiö E, Kärenlampi S O, Laiho A, Aarts M G M, Pursiheimo J P, Schat H, Schmidt H, Tuomainen M H, Tervahauta A I. Gene expression differences between Noccaea caerulescens ecotypes help to identify candidate genes for metal phytoremediation[J]. Environmental Science & Technology, 2014, 48(6): 3344-3353.doi:10.1021/es4042995. doi: 10.1021/es4042995 URL
[17]	Tripathi D K, Singh S, Gaur S, Singh S, Yadav V, Liu S L, Singh V P, Sharma S, Srivastava P, Prasad S M, Dubey N K, Chauhan D K, Sahi S. Acquisition and homeostasis of iron in higher plants and their probable role in abiotic stress tolerance[J]. Frontiers in Environmental Science, 2018, 5: 86.doi: 10.3389/fenvs.2017.00086. doi: 10.3389/fenvs.2017.00086 URL
[18]	Worthington M, Perez J G, Mussurova S, Silva-Cordoba A, Castiblanco V, Cardoso Arango J A, Jones C, Fernandez-Fuentes N, Skot L, Dyer S, Tohme J, di Palma F, Arango J, Armstead I, de Vega J J. A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses[J]. Journal of Experimental Botany, 2020, 72(2): 302-319.doi:10.1093/jxb/eraa469. doi: 10.1093/jxb/eraa469 URL
[19]	Nakano Y, Kusunoki K, Hoekenga O A, Tanaka K, Iuchi S, Sakata Y, Kobayashi M, Yamamoto Y Y, Koyama H, Kobayashi Y. Genome-wide association study and genomic prediction elucidate the distinct genetic architecture of aluminum and proton tolerance in Arabidopsis thaliana[J]. Frontiers in Plant Science, 2020, 11: 405.doi:10.3389/fpls.2020.00405. doi: 10.3389/fpls.2020.00405 pmid: 32328080
[20]	Ghimire B, Saminathan T, Bodunrin A, Abburi V L, Kshetry A O, Shinde S, Nimmakayala P, Reddy U K. Genome-wide association study of natural variation in Arabidopsis exposed to acid mine drainage toxicity and validation of associated genes with reverse genetics[J]. Plants, 2021, 10(2):191.doi: 10.3390/plants10020191. doi: 10.3390/plants10020191 URL
[21]	Kusunoki K, Nakano Y, Tanaka K, Sakata Y, Koyama H, Kobayashi Y. Transcriptomic variation among six Arabidopsis thaliana accessions identified several novel genes controlling aluminium tolerance[J]. Plant,Cell & Environment, 2017, 40(2): 249-263.doi:10.1111/pce.12866. doi: 10.1111/pce.12866
[22]	Mangeon A, Pardal R, Menezes-Salgueiro A D, Duarte G L, de Seixas R, Cruz F P, Cardeal V, Magioli C, Ricachenevsky F K, Margis R, Sachetto-Martins G. AtGRP3 is implicated in root size and aluminum response pathways in Arabidopsis[J]. PLoS One, 2016, 11(3): e0150583.doi:10.1371/journal.pone.0150583. doi: 10.1371/journal.pone.0150583 URL
[23]	Ojeda-Rivera J O, Oropeza-Aburto A, Herrera-Estrella L. Dissection of root transcriptional responses to low pH,aluminum toxicity and iron excess under pi-limiting conditions in Arabidopsis wild-type and stop1 seedlings[J]. Frontiers in Plant Science, 2020, 11: 01200.doi:10.3389/fpls.2020.01200. doi: 10.3389/fpls.2020.01200 pmid: 33133111
[24]	Yang L B, Fan T T, Guan L X, Ren Y B, Han Y, Liu Q, Liu Y S, Cao S Q. CMDH4 encodes a protein that is required for lead tolerance in Arabidopsis[J]. Plant and Soil, 2017, 412(1/2): 317-330.doi:10.1007/s11104-016-3078-8. doi: 10.1007/s11104-016-3078-8 URL
[25]	Zandalinas S I, Sengupta S, Burks D, Azad R K, Mittler R. Identification and characterization of a core set of ROS wave-associated transcripts involved in the systemic acquired acclimation response of Arabidopsis to excess light[J]. The Plant Journal, 2019, 98(1): 126-141.doi:10.1111/tpj.14205. doi: 10.1111/tpj.14205 pmid: 30556340
[26]	Richards K D, Schott E J, Sharma Y K, Davis K R, Gardner R C. Aluminum induces oxidative stress genes in Arabidopsis thaliana[J]. Plant Physiology, 1998, 116(1): 409-418.doi:10.1104/pp.116.1.409. doi: 10.1104/pp.116.1.409 pmid: 9449849
[27]	Papdi C, Ábrahám E, Joseph M P, Popescu C, Koncz C, Szabados L. Functional identification of Arabidopsis stress regulatory genes using the controlled cDNA overexpression system[J]. Plant Physiology, 2008, 147(2): 528-542.doi:10.1104/pp.108.116897. doi: 10.1104/pp.108.116897 URL
[28]	Yamaji N, Huang C F, Nagao S, Yano M, Sato Y, Nagamura Y, Ma J F. A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice[J]. The Plant Cell, 2009, 21(10): 3339-3349.doi:10.1105/tpc.109.070771. doi: 10.1105/tpc.109.070771 pmid: 19880795
[29]	López-Galiano M J, González-Hernández A I, Crespo-Salvador O, Rausell C, Real M D, Escamilla M,Camañes G,García-Agustín P,González-Bosch C,García-Robles I. Epigenetic regulation of the expression of WRKY75 transcription factor in response to biotic and abiotic stresses in Solanaceae plants[J]. Plant Cell Reports,2018,37(1): 167-176.doi:10.1007/s00299-017-2219-8. doi: 10.1007/s00299-017-2219-8
[30]	Ma H X, Bai G H, Carver B F, Zhou L L. Molecular mapping of a quantitative trait locus for aluminum tolerance in wheat cultivar Atlas 66[J]. Theoretical and Applied Genetics, 2005, 112(1): 51-57.doi:10.1007/s00122-005-0101-5. doi: 10.1007/s00122-005-0101-5 pmid: 16189660
[31]	Cai Z D, Cheng Y B, Xian P Q, Lin R B, Xia Q J, He X K, Liang Q W, Lian T X, Ma Q B, Nian H. Fine-mapping QTLs and the validation of candidate genes for aluminum tolerance using a high-density genetic map[J]. Plant and Soil, 2019, 444(1/2): 119-137.doi:10.1007/s11104-019-04261-0. doi: 10.1007/s11104-019-04261-0 URL
[32]	王瑞莉, 王刘艳, 叶桑, 郜欢欢, 雷维, 吴家怡, 袁芳, 孟丽姣, 唐章林, 李加纳, 周清元, 崔翠. 铝毒胁迫下甘蓝型油菜种子萌发期相关性状的QTL定位[J]. 作物学报, 2020, 46(6): 832-843.doi:10.3724/SP.J.1006.2020.94154. doi: 10.3724/SP.J.1006.2020.94154
	Wang R L, Wang L Y, Ye S, Gao H H, Lei W, Wu J Y, Yuan F, Meng L J, Tang Z L, Li J N, Zhou Q Y, Cui C. QTL mapping of seed germination-related traits in Brassica napus L.under aluminum toxicity stress[J]. Acta Agronomica Sinica, 2020, 46(6): 832-843. doi: 10.3724/SP.J.1006.2020.94154 URL
[33]	Kobayashi Y, Furuta Y, Ohno T, Hara T, Koyama H. Quantitative trait loci controlling aluminium tolerance in two accessions of Arabidopsis thaliana (Landsberg erecta and Cape Verde Islands)[J]. Plant,Cell & Environment, 2005, 28(12):1516-1524.doi: 10.1111/j.1365-3040.2005.01388.x. doi: 10.1111/j.1365-3040.2005.01388.x
[34]	Sharma A D, Sharma H, Lightfoot D A. The genetic control of tolerance to aluminum toxicity in the Essex by Forrest recombinant inbred line population[J]. Theoretical and Applied Genetics, 2011, 122(4): 687-694.doi:10.1007/s00122-010-1478-3. doi: 10.1007/s00122-010-1478-3 pmid: 21060987
[35]	Kochian L V, Piñeros M A, Liu J P, Magalhaes J V. Plant adaptation to acid soils: The molecular basis for crop aluminum resistance[J]. Annual Review of Plant Biology, 2015, 66: 571-598.doi:10.1146/annurev-arplant-043014-114822. doi: 10.1146/annurev-arplant-043014-114822 pmid: 25621514
[36]	Liu J P, Piñeros M A, Kochian L V,. The role of aluminum sensing and signaling in plant aluminum resistance[J]. Journal of Integrative Plant Biology,2014,56(3): 221-230.doi:10.1111/jipb.12162. doi: 10.1111/jipb.12162
[37]	丁戈, 黄杨, 陈伦林, 李书宇, 宋来强, 熊洁. 基于转录组测序的铝胁迫下甘蓝型油菜新内参基因的发掘与引物开发[J]. 华北农学报, 2021, 36(1): 1-9. doi: 10.7668/hbnxb.20191565. doi: 10.7668/hbnxb.20191565
	Ding G, Huang Y, Chen L L, Li S Y, Song L Q, Xiong J. RNA-seq based discovery of new reference genes and primers in Brassica napus under aluminum stress[J]. Acta Agriculturae Boreali-Sinica, 2021, 36(1): 1-9.
[38]	Daspute A A, Sadhukhan A, Tokizawa M, Kobayashi Y, Panda S K, Koyama H. Transcriptional regulation of aluminum-tolerance genes in higher plants: Clarifying the underlying molecular mechanisms[J]. Frontiers in Plant Science, 2017, 8: 1358.doi:10.3389/fpls.2017.01358. doi: 10.3389/fpls.2017.01358 pmid: 28848571
[39]	de Michele R, Vurro E, Rigo C, Costa A, Elviri L, di Valentin M, Careri M, Zottini M, Sanità di Toppi L, Lo Schiavo F. Nitric oxide is involved in cadmium-induced programmed cell death in Arabidopsis suspension cultures[J]. Plant Physiology, 2009, 150(1): 217-228.doi:10.1104/pp.108.133397. doi: 10.1104/pp.108.133397 URL

性状 Traits	金黄麻 JHM	竹山白 ZSB	RIL群体平均值 RIL mean	RIL群体变幅 RIL range
相对根长Relative root length	0.58	0.19	0.57	0.21~1.01
相对芽长Relative shoot length	0.78	0.39	0.81	0.53~1.01
相对苗鲜质量Relative fresh seedling weight	0.78	0.41	0.77	0.47~1.01

性状 Traits	金黄麻 JHM	竹山白 ZSB	RIL群体平均值 RIL mean	RIL群体变幅 RIL range
相对根长Relative root length	0.58	0.19	0.57	0.21~1.01
相对芽长Relative shoot length	0.78	0.39	0.81	0.53~1.01
相对苗鲜质量Relative fresh seedling weight	0.78	0.41	0.77	0.47~1.01

性状 Traits	相对根长 RRL	相对芽长 RSL	相对苗鲜质量 RSW
相对根长RRL	1.000
相对芽长RSL	0.646^**	1.000
相对苗鲜质量RSW	0.517^**	0.532^**	1.000

性状 Traits	相对根长 RRL	相对芽长 RSL	相对苗鲜质量 RSW
相对根长RRL	1.000
相对芽长RSL	0.646^**	1.000
相对苗鲜质量RSW	0.517^**	0.532^**	1.000

性状 Traits	QTL	染色体 Chromosome	标记区间 Marker interval	LOD	加性效应 Additive effect	表型贡献率/% R²
相对根长	qRRL2	Chr2	c02b009~c02b013	3.24	0.05	7.01
RRL	qRRL10	Chr10	c10b018~c10b019	2.63	-0.05	5.92
	qRRL11	Chr11	c11b024~c11b025	2.55	0.04	5.46
相对芽长	qRSL6	Chr6	c06b062~c06b066	3.55	0.03	7.75
RSL	qRSL13	Chr13	c13b079~c13b080	3.12	0.02	6.68
相对苗鲜质量	qRSW2	Chr2	c02b008~c02b010	2.68	0.02	5.85
RSW	qRSW4	Chr4	c04b064~c04b065	2.55	-0.03	5.95

选择文件类型/文献管理软件名称

选择包含的内容