摘要： 为发掘大麦株高类性状的QTL位点，改良大麦品种株高性状。以我国饲用大麦泰兴9425与日本啤酒大麦Naso Nijo构建的177份DH群体及亲本为材料，考查2种环境下参试材料的株高、穗下节间长、穗长3个株高类性状，结合已构建的分子标记连锁图谱，采用基于完备区间作图法的Windows QTL IciMaping V4.1.0.0软件进行QTL定位分析并分析株高类性状的遗传特性。结果表明：株高、穗长、穗下节间长主要受遗传因素控制，同时受试点的生态条件和生产条件影响，3个株高类性状的遗传力分别为78.98%，89.31%，84.50%。株高与穗长、株高与穗下节间长、穗长与穗下节间长均呈极显著正相关，相关系数分别为0.688，0.862和0.600，表明株高越高其穗长、穗下节间长越长。2个试点定位到6个株高QTL、5个穗长QTL及4个穗下节间长QTL，共15个QTL，除第1，6号染色体外，其他染色体上均有分布，LOD值为3.50~32.46，对表型变异的解释率为1.53%~38.30%。其中qPH-4-1、qPH-7-2、qSL-2-1、qSL-2-2、qSIL-2-1、qSIL-3-2均未见报道，可能为新位点，为大麦株高类性状的改良与分子标记辅助育种奠定基础。

关键词: 大麦, 株高类性状, QTL定位, DH群体, 遗传分析

Abstract: In order to discover the QTL of barley plant height-related characters, improve the barley plant height-related characters. Chinese forage barley Taixing 9425 and Japanese beer barley Naso Nijo as well as 177 doubled haploid lines, generated from a cross between Taixing 9425 and Naso Nijo, were used as materials to examine the three plant traits of plant height(PH), internode length under spike(SIL), and spike length(SL) in two environments. Characters, combined with the constructed molecular marker linkage map, using Windows QTL IciMaping V4.1.0.0 software based on the complete interval mapping method for QTL mapping analysis, also the genetic characteristics were analyzed. The results showed that:plant height, spike length, and internode length under spike were mainly controlled by genetic factors, and at the same time affected by the ecological conditions and production conditions of the pilot. The heritability of the three traits was 78.98%, 89.31% and 84.50%, respectively; Plant height was extremely significant positive correlation with the spike length and the internode length under the spike. Plant height and spike length, plant height and the internode length under the spike, and spike length and the internode length under the spike were all significantly positively correlated, with correlation coefficients of 0.688, 0.862 and 0.600, respectively, indicating that the higher the plant height, the longer the spike length and the internode length under the spike. The 2 pilot sites mapped 6 QTL for plant height, 5 QTL for spike length, and 4 QTL for internode length under spike. A total of 15 QTL are located on all chromosomes except chromosomes 1 and 6. The LOD value was 3.50-32.46, the explanation rate of phenotypic variation was 1.53%-38.30%. Among them, qPH-4-1, qPH-7-2, qSL-2-1, qSL-2-2, qSIL-2-1 and qSIL-3-2 have not been reported, and may be new loci. This lay the foundation for the improvement of barley plant height-related characters and molecular marker assisted breeding.

Key words: Barley, Height-related characters, QTL mapping, Double haploid line population, Genetic analysis

中图分类号: 

• S512.03

• Q78